THE EFFECT OF TOMAN FISH EXTRACT (Channa Micropeltes) ONNEUTROPHILIN DIABETES MELLITUSWOUND HEALING (In Vivo Study in the Back of Male Wistar Mice (Ratus Novergicus)

Nuril Fajriani, Amy Nindia Carabelly, Maharani Laillyza Apriasari

Abstract


Background: Toman fishcontains albumin and unsaturated fatty acids which can be an alternative of diabetes mellitus wounds healing process. People with diabetes mellitus have their neutrophil decreased. Albumin increases the number of neutrophil. Unsaturated fatty acid decreases the number of neutrophil. Purpose: To prove the effect of toman fish extract of 16 ml/Kg BW mice orally on thenumber of neutrophil in day 2, 4, and 8 of male wistarmice with diabetic wound. Materials and Method: This research applied pure experimental research method with posttest only control design. This research took 36 wistarmice which weredivided into negative control group which is given BR2 feed, treatment group which is giventoman fish extract in 16 mL/Kg BW mice and positive control group which is given haruan fish extract in 13.54 mL/Kg BW mice. The Result: One-way ANOVAshowedthat there wassignificant differenceof all groups in day 2 (p=0.000), day 4 (p=0.000) and day 8 (p=0.000). Post-Hoc LSD test for the average of neutrophil number showedthat there was significant difference (p<0.05) between the negative control group, treatment group and the positive control group. Conclusion: Toman fish extract has an effect which increasesthe number ofneutrophil in day 2 and decreasesthe number of neutrophil in day 4 and 8 on the healing of diabetic wound in mice.

Keywords


diabetes mellitus wound healing; number of neutrophil; toman fish extract.

Full Text:

PDF

References


Greenberg M.S, Glick M, Ship J.A. 2008. Burkert’s Oral Medicine.11st Ed. Hamilton: Bc Decker Inc; 2007. p. 509.

Price S.A, Wilson M.L. Patofisiologi Konsep Klini Proses-Proses Penyakit. Jakarta: EGC; 2015. Hlm. 35-36.

Kementerian Kesehatan RI. Riset Kesehatan Dasar (RISKESDAS). Jakarta: Balitbang Kemenkes RI; 2013.hlm. 64.

International Diabetes Federation (IDF). Atlas Diabetes. 6thEd. United Kingdom: International Diabetes Federation ; 2017 p. 109.

Soegondo S, SuwondoP, Subekti L. Diagnosis dan Klasifikasi Diabetes Mellitus Terkini. Jakarta: Balai Penerbit FKUI; 2007.Hlm. 18.

Ciptanto S.TOP 10 Ikan Air Tawar. Yogyakarta : Lily Publisher; 2010. Hlm.138-143.

Santoso A.H. Potential of snakehead (channastiata) extract as hepatoprotector on paracetamol induced rat. Bogor : Institute of Agriculture; 2009. Hlm. 20-26.

Nicodemus, Andrie M, Luliana S. Uji Efek Penyembuhan Luka Sayat Ekstrak Ikan Toman (Channamicropeltes) secara Oral pada Tikus Putih Jantan Wistar. Jurnal UNTAN. 2014; 1(1): 12.

Firlianty, Supriyatno E, Nursyam H, Hardoko, Mustafa A. Chemical Composition and Amino Acid Profile of Channidae Collected From Central Kalimantan, Indonesia. International Journal of Science and Technology (IJSTE). 2013; 2(4): 25-29.

Omar M.N, Nur-shahidatul A.Y, Nur‘aziyah Z, Ahmad M.Z. Bioconversion of ω-Fatty Acid from Giant Snakehead (Channamicropeltes) Fish Oil. Oriental Journal of Chemistry.2014; 30 (3): 1133-1136.

Diana J, Simoni Y, Furio L, Beaudoin L, Agerberth B, Barrat F, Lehuen A. Crosstalk between neutrophils, B-1a cells and plasmacytoid dendritic cells initiates autoimmune diabetes. Nat Med.2013; 19 (1): 65 – 73.

Serhan C.N. Resolution Phase of Inflammation: Novel Endogenous Anti-Inflammatory and Proresolving Lipid Mediators and Pathways. Annu. Rev. Immunol. 2007; 25 (1): 101–137.

Winarsih W, Ietje W, Lina N.S. Aktivitas Salep Ekstrak Rimpang Kunyit dalam Proses Persembuhan Luka pada Mencit yang Diinduksi Diabetes. Jurnal Veteriner. 2012; 13 (3): 242-250.

Murdani O.J, Mohhamd A, Wintari T. Uji Efek Penyembuhan Luka Sayat Ekstrak Ikan Toman (Channamicropeltes) Secara Oral Pada Tikus Jantan Galur Wistar Yang Diinduksi Streptozotocin. Jurnal Mahasiswa Farmasi UNTAN. 2017; 1 (1) : 1-9.

Akbar J.Potensi Dan Tantangan Budidaya Ikan Rawa (Ikan Hitaman Dan Ikan Putihan) Di Kalimantan Selatan. Banjarmasin:Unlam Press; 2014.Hlm. 92-93.

Gufran H.M, Kordi. K. Panduan Lengkap Memelihara Ikan Air Tawar di Kolam Terpal. Yogyakarta : Lily Publisher; 2009. Hlm. 152.

Suhartono E, Triawanti, Yunanto A, Firdaus R.T, Iskandar. Chronic Cadmium Hepatooxidative in Rats: Treatment with Haruan fish (Channastriata) Extract. Amsterdam: Elsevier; 2012. p. 1-5.

Agustin R, Dewi N, Rahardja D.S. Efektivitas Ekstrak Ikan Haruan (Channastriata) dan Ibuprofen Terhadap Jumlah Sel Neutrofil Pada Proses Penyembuhan Luka Studi In Vivo Pada Mukosa Bukal Tikus (Ratus norvegicus) Wistar. Dentiono (Jur. Ked. Gigi).2016; 1 (1): 68-74.

Putri R.C.Sdan Agustina W. Pengaruh Pemberian Ekstrak Albumin Ikan Gabus (Channastriata) Topikal Terhadap Pecerpatan Kontraksi Luka Insisi Pada Tikus Putih (Ratusnorvegicus) Strain Wistar. Journal of Nursing Care & Biomolecular. 2016; 1 (1): 45-50.

Mustafa A, Sajuti H, Permatasari N, Widodo M.A.The Effect of Channastriata Extract on Total Amino Acid, Arginine, and Leucine Consentration in Serum of Streptozotocin Induced Diabetic Rat. International Journal of Science and Technology (IJSTE).2014; 3 (4): 22-27.

Deindl E, Christian K.Therapeutic Neovascularization: Quo Vodis?. Netherlands: Springer; 2007. p. 52.

Mansur A.R. Merawat Luka Komplikasi Diabetes. DalamHafid, editor. Majalah Kesehatan Muslim Diabetes Mellitus, edisi ke-9. Yogyakarta: Pustaka Muslim; 2013. Hlm. 31.

Ponugoti B, Xu F, Zhang C, Tian C, Pacios S, Graves D.T. FOXO1 promotes wound healing through the up-regulation of TGF-beta1 and prevention of oxidative stress. J Cell Biol. 2013; 203(2): 327–43.

Björnsdóttir H. Intracellular Radicals in Neutrophils. Gothenburg, Sweden: Department of Rheumatology and Inflammation Research Institute of Medicine Sahlgrenska Academy at University of Gothenburg; 2015.p. 13-30.

Narwadiya S.C, Dhumne U.L, Sahare K.N, Tuman P.M, Meshram V.G, Singh V. Serum Protein Level Changes in Dots Administered Patients of Nagpur District: A Case Study. India Asian J Exp BiolScienst.2012; 3 (1): 251-254.

Suhartono E, Djati M.S. Radikal Bebas dan Intoksikasi Kadmium. Banjarmasin: Pustaka Banua ; 2014. Hlm. 7-21.

Standring S. Gray’s AnatomyThe Anatomical Basic Of Clinical Practice.41stEd. London UK: Elsevier; 2016. p. 70.

Rahmawati G, Rachmawati F.N danWinarsih H. Aktivitas Superokisda Dismutase Tikus Diabetes yang Diberi Ekstrak Batang Kapulaga dan Glibenklamid. Jurnal Scripta Biologica. 2014; 1 (3): 197-201.

Fisheries and Marine Services of Central Kalimantan Tengah. Fish Resources Profile of Central Kalimantan. Ed. Ke-2. Jakarta: Fisheries Marine Services; 2009. p. 12-13.

Firliyanti.Vacuum Drying Albumin Powder of Snakehead (Channamicropeltes) Potential For Wound Healing from Central Kalimantan, Indonesia. International Journal of ChemTech Research. 2016; 9 (6): 283-289.

Kumar V, Cotran R.S dan Robbins S.L. Buku ajar patologi. Edisi 7. Vol 1. Jakarta: EGC; 2007.Hlm. 45-70.

Sherwood L. Fisiologi Manusia dari sel ke sistem. Ed.6. Jakarta: EGC; 2014.Hlm. 45.

Sen C, Roy S. Redox signals in wound healing. BiochimBiophys Acta.2008; 1780 (11): 1348.

Tie L, Yang H.Q, An Y, Liu S.Q, Han J,et al. Ganoderma Lucidum Polysaccharide Accelerates Refractory Wound Healing by Inhibition of Mitochondrial Oxidative Stress in Type 1 Diabetes. Cell PhysiolBiochem. 2012; 29 (1): 583–594.

Zgheib C, Maggie M, Hodges, Junyi H, Kenneth W.L, Junwang X. Research Artickel Long non-coding RNA Lethe Regulates Hyperglycemia-Induced Reactive Oxygen Species Production in Macrophages. PLOS One.2017; 11 (1): 9.

Masaki O, Victor T.G.C.Human serum Albumin: A Multifunctional Protein. Dalam. Albumin in Medicine: Pathological and Clinical Applications. Singapore: Springer; 2016. p. 19.

Suhartono E. Toksisitas Oksigen Reaktif & Antioksidan di Bidang Kedokteran dan Kesehatan. Yogyakarta: Gosyen Publishing; 2016. Hlm. 81.

Pradana A.R.A, Mansur I, Muhammad S.J, Muhaimin R. Modulation of Granulocyte Cell Development by Vip Albumin® Administration in BALB/C Mice with Diabetes Mellitus. The Journal Of Tropical Life Science.2015; 5 (3): 133-140.

Mittal M, Mohammad R.S, Khiem T, Sekhar P, Asrar B.M. Reactive Oxygen Species in Inflammation and Tissue Injury. Antioxidant and Redox Signaling. 2014; 20 (7): 1126-1127.

Peiris H, Claudine S, Bonder P, Toby H, Coates, Damien J, Keating, Claire F, Jessup. The b-Cell/EC Axis: How Do Islet Cells Talk to Each Other?. Diabetes. 2014; 63 (1): 3.

Khatami M.Inflammation, Chronic Diseases and Cancer- Cell and Molleculer Biology, Immunology and Clinical Base. Philadelphia: Intech; 2012.p. 145.

Ngui W.S.Y, Nur H.H, Nadiah R, Saiful I.Z. Malaysia Snakehead ChannaStriatus and Micropeltes: Physico-chemical Properties of Fillet Fish Oil and Water-soluble Extract. Chemical Engineering Transactions.2017; 56 (1): 61-66.

SerhanC.N, Ward P.A, Gilroy D.W. Fundamental Of Inflammation. New York USA: Cambridge University Press; 2010. p. 170.

Simopoulos A.P. Review An Increase in the Omega-6/Omega-3 Fatty Acid Ratio Increases the Risk for Obesity. Nutrients. 2016; 8 (128): 1-17.

Ganong W.F. Buku Ajar Fisiologi Kedokteran. Edisi 22. Jakarta: EGC; 2013. Hlm. 534-536.

Rosales C, Demaurex N, Clifford A, Lowell, Uribe-Querol E. Editorial Neutrophils: Their Role in Innate and Adaptive Immunity. Journal of Immunology Research.2016; 1 (1): 1-2.

Handaya A.Y. Tepatdan Jitu Atasi Ulkus Kaki Diabetes. Yogyakarta: Rapha; 2016.Hlm. 29-31.

Koppaka S, Kehlenbrink S dan Carey M. Reduced Adipose Tissue Macrophage Content is Associated With Improved Insulin Sensitivity In Thiazolidinedione-Treated Diabetic Humans. Diabetes. 2013; 62 (1): 1843–54.

Delavary M.B, Van D V, Van E, M, Niessen F.B, Beelen R.H. Macrophages in Skin Injury and Repair. Immunobiology. 2012; 216 (1): 753–762.

Menghini R, Casagrande V, Menini S. TIMP3 Overexpression in Macrophages Protects From Insulin Resistance, Adipose Inflammation, and Nonalcoholic Fatty Liver Disease in Mice.Diabetes. 2015; 61 (1) : 454–62.

Zhao R, Helena L, Elizabeth C, Christopher J, Meilang X. Review Inflammation in Chronic Wounds. International Journal of Mollecular Sciences. 2016; 17 (2085): 1-14.

Le T, BushanV.First AIDS for the USMLE Step 1. Philadelphia: Mc Graw Hill Professional ; 2013.p. 216.

Hesketh M, Katherine B, Sahin, Zoe E, West, Rachael Z, Murray. Review Macrophage Phenotypes Regulate Scar Formationand Chronic Wound Healing. Int. J. Mol. Sci.2017. 18 (1): 1545.

Gurtner G.C, Thorme C.H. Wound healing: Normal and abnormal.6thEd. Grabb and Smith’s plastic surgery; 2007. p. 12-15.

Sharifan, Z,etal. Histological and Gene Expression Analysis of The Efects of Pulsed Low-Level Laser Therapy on Wound Healing of Streptozotocin-Induced Diabetic Rats. Lasers Med Sci.2014; 29 (1): 1227–1235.

Harsunen M.H, Puff R, D’Orlando O. Reduced blood leukocyte and neutrophil numbers in the pathogenesis of type 1 diabetes. Horm Metab Res. 2013; 45 (1): 467–470.

Valle A, Giamporcaro G.M, Scavini M. Reduction of circulating neutrophils precedes and accompanies type 1 diabetes. Diabetes. 2013; 62 (1): 2072–2077.

Wang Y, Xiao Y, Zhong L.Increased neutrophil elastase and proteinase 3 and augmented NETosis are closely associated with beta-cell autoimmunity in patients with type 1 diabetes. Diabetes. 2014; 63 (1): 4239–4248.

Kewcharoenwong C, Darawan R, Arnone N, Gregory J.B, Manabu A, Ganjana L. Glibenclamide Impairs Responsesof Neutrophils Against Burkholderiapseudomalleiby Reduction of Intracellular Glutathione. Scientific Reports. 2014; 1 (1): 1-12.

Mikhalchika E.V, Smolinaa N.V, Astamirova T.S, Gorudko I.V, Grigorieva D.V, Ivanov V.A, SokolovA.V, Kostevicha V.A, Cherenkevich S.N, Panasenko O.M. Human Serum Albumin Modified under Oxidative/ Halogenative Stress Enhances Luminol Dependent Chemiluminescence of Human Neutrophils. Biophysics. 2013; 58 (4); 681-689.

Gartner L.P, Hiatt J.L.Concise Histology. Philadelphia : Elsavier; 2010. p. 89-110.

Gartner L.P, Hiatt J.L.Color and Text of Histology.6th Ed. Philadelphia : Wolter Kluwer Health Lippincott William dan Wilkins; 2014. p. 109-112.


Article Metrics

Abstract view : 310 times
PDF - 236 times

Refbacks

  • There are currently no refbacks.


 

Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia
Telp/Fax. (0511) 3255444;

E-mail. fkg@ulm.ac.id / dentino.ulm@gmail.com
Website. fkg.ulm.ac.id

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.