Penambatan Molekul Kandungan Eurycoma longifolia Jack. (Pasak bumi) terhadap Human Phosphodiesterase 5

Samsul Hadi, Khoerul Anwar, Amalia Khairunnisa, Noer Komari

Abstract


Pasak bumi sebagai obat tradisional pria telah digunakan oleh masyarakat melayu, khususnya orang Sumatra dan kalimantan, akan tetapi mekanisme secara molekuler belum dikatahui dengan jelas. Salah satu pendekatan yang dapat digunakan untuk mengetahui mekanisme pasak bumi sebagai obat tradisional yang bermanfaat mengatasi disfungsi ereksi adalah penambatan molekul. Penambatan melekul dilakukan dengan cara melihat interaksi ligand dengan reseptor. Ligand yang berasal dari pasak bumi adalah 5-methoxycanthin-6-one, 9-methoxycanthin-6-one, eurycomalactone, eurycomalide A, eurycomanone, eurycomaoside, laurycolactone A, longilactone, niloticin, picrasidine O. Sehingga kesepuluh ligand ini didocking menggunakan salah satu aplikasi docking yaitu PLANTS 1.1 untuk melihat ikatan dengan PDE5. Sebelum dilakukan docking terhadap ligand pasak bumi, terlebih dahulu dilakukan docking terhadap ligand Sildenafil untuk melhat nilai RMSD. RMSD ini diperlukan sebagai alat validasi metode, berdasarkan percobaan, nilai RMSD dari Sildenafil adalah 1,2517 A0. Hasil terbaik dari penambatan molekul pasak bumi adalah niloticin dengan skor doking -97,8802, sehingga nilai interaksinya terhadap reseptor sebesar 93,71% dari Sildenafil.

Pasak bumi as traditional male medicine has been used by Malay people, especially Sumatra and Borneo people, but the molecular mechanism is not yet known clearly. One approach that can be used to determine the mechanism of the Pasak Bumi as a traditional medicine that is useful to overcome erectile dysfunction is docking molecules. Molecular docking is done by looking at the interaction of ligands with receptors. Ligands originating from the Pasak Bumi are 5-methoxycanthin-6-one, 9-methoxycanthin-6-one, eurycomalactone, eurycomalide A, eurycomanone, eurycomaoside, laurycolactone A, longilactone, niloticin, picrasidine O. docking application namely PLANTS 1.1 to see the bond with PDE5. Before docking Pasak Bumi ligand, it is first docking Sildenafil ligand to see the RMSD value. The RMSD is needed as a method validation method, based on experiments, the RMSD value of Sildenafil is 1.2517 A0. The best results from docking of the Pasak bumi molecule is niloticin with a doctor score of -97.8802, so interaction skor to the receptor is 93.71% of Sildenafil.

Keywords: Pasak Bumi, PDE5, PLANTS


Keywords


Pasak bumi, PDE5, PLANTS

Full Text:

PDF

References


Beavo, J. A. 1995.Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms. Physiol. Rev, 75, 725–748.

Corbin, J. D. & Francis, S. H. 2002.Pharmacology of phosphodiesterase-5 inhibitors. Int. J. Clin. Pract, 56, 453–459

Hajjouli, S., S. Chateauvieux., M. H. Teiten., B. Orlikova., M. Schumacher., M. Dicato., C. Y. Choo, & M. Diederich. 2014. Eurycomanone and Eurycomanol from Eurycoma longifolia Jack as Regulators of Signaling Pathways Involved in Proliferation, Cell Death and Inflammation.Molecules. 17: 1-7.

Heriyanto, N. M., R. Sawitri, & E. Subiandono. 2006. Kajian Ekologi dan Potensi Pasak Bumi (Eurycoma longifolia Jack.) di Kelompok Hutan Sungai Manna-Sungai Nasal, Bengkulu. Buletin Plasma Nutfah. 12: 69-75.

Jeffrey, P. D. 1995.Mechanism of CDK activation revealed by the structure of a cyclinA–CDK2complex. Nature ,376, 313–320

Korb O, Stützle T, dan Exner TE. Ant Colony Optimization Approach to Flexible Protein-Ligand Docking. Swarm Intell. 2007. hal : 115-134.

Prasojo SL, Hartanto FA, Yuniarti N, Ikawati Z, Istyastono EP. 2010 Docking of 1- henylsulfonamide-3Trifluoromethyl-5-parabromophenyl-pyrazole to cyclooxygenase-2 using PLANTS. Indonesian Journal of Chemistry. 10(3):348-51.

Purnomo H., 2019.Molecular Docking Parasetamol dan Analognya menggunkan PLANTS (Protein-Ligand ANT-System). Rapha Publishing. Yogyakarta.

Rotella, D. P. 2002.Phosphodiestease 5 inhibitors: current status and potential applications. Nature Rev.Drug Discov, 1, 674–682.

Rybalkin, S. D., Rybalkina, I. G., Shimizu-Albergine, M., Tang, X. B. & Beavo, J. A. 2003.PDE5 is converted to an activated state upon cGMP binding to the GAF A domain. EMBO J, 23, 469–478

Sung, B.-J., Hwang, K.Y., Jeon, Y.H., Lee, J.I., Heo, Y.-S., Kim, J.H., Moon, J., Yoon, J.M., Hyun, Y.-L., Kim, E., Eum, S.J., Park, S.-Y., Lee, J.-O., Lee, T.G., Ro, S., Cho, J.M. 2003.Structure of the Catalytic Domain of Human Phosphodiesterase 5 With Bound Drug Molecules. Nature. 4;425(6953):98-102.

Tee, B. H., Hoe, S. Z., Cheah, S. H., & Lam, S. K. (2017). Effects of Root Extracts of Eurycoma longifolia Jack on Corpus Cavernosum of Rat. Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre, 26(3), 258–265. https://doi.org/10.1159/000464363

Thu, H. E., Mohamed, I. N., Hussain, Z., Jayusman, P. A., & Shuid, A. N. (2017). Eurycoma Longifolia as a potential adoptogen of male sexual health: a systematic review on clinical studies. Chinese Journal of Natural Medicines, 15(1), 71–80. https://doi.org/10.1016/S1875-5364(17)30010-9

Vejayan, J., Mohamed, A., Zulkifli, A., Yahya, Y., Munir, N., & Yusoff, M. (2018). Marker to Authenticate Eurycoma Longifolia (Tongkat Ali) Containing Aphrodisiac Herbal Products. Current Science, 115, 886. https://doi.org/10.18520/cs/v115/i5/886-894




DOI: http://dx.doi.org/10.20527/jps.v7i2.8731

Article Metrics

Abstract view : 98 times
PDF - 90 times

Refbacks

  • There are currently no refbacks.


Jurnal Pharmascience Published by:

Program Studi Farmasi Universitas Lambung Mangkurat

Banjarbaru, Indonesia

 

Jurnal Pharmascience is indexed by:

      

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.