KOMBINASI AIR KELAPA DAN EKSTRAK TOMAT PADA MEDIA GROWMORE UNTUK SUBKULTUR ANGGREK Dendrobium candy strip lasianthera (CL)

Ade Putri, Rita Ningsih, Sri Ambardini

Abstract


This study aims to determine the effects of individual and combined concentrations of coconut water and tomato extract as natural plant growth regulators (PGRs) on the in vitro growth of Dendrobium candy strip lasianthera (CL) plantlets cultured on Growmore subculture medium. Shoot explants originating from sterile cultures were selected, with an approximate size of 0.5 cm. The experiment employed a completely randomized design (CRD) with a two-factor treatment: tomato extract (T) concentration consisting of four levels (0, 10, 20, and 30%) and coconut water (K) at four levels (0, 50, 75, and 100 mL/L), each with five replications. Observations were conducted over an 8-week period. Data were analyzed using a Two-Way Analysis of Variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT) at a 5% significance level. The results indicated that the single treatment of tomato extract significantly affected the number of leaves and shoots. The single treatment of coconut water had a significant effect on the number of roots. Furthermore, the combination of coconut water and tomato extract significantly influenced plantlet height and shoot length. The optimal single tomato extract treatments were 30% (T3), producing an average of 3.8 leaves, and 10% (T1), producing an average of 2.6 shoots. The most effective single coconut water treatment was 100 mL/L (K3), which produced an average of 3.05 roots. The most effective combination treatments were 100 mL/L coconut water with 30% tomato extract (K3T3), resulting in a plantlet height of 1.36 cm, and 50 mL/L coconut water with 30% tomato extract (K1T3), resulting in a shoot length of 1.2 mm.


Full Text:

PDF

References


Amalia A, Mubarok S, Nuraini A. 2022. Respons anggrek Dendrobium terhadap perbedaan naungan dan aplikasi zat pengatur tumbuh. Jurnal Kultivasi. 21(2): 127–134.

Ambarwati I, Alfian F, Dewanti P. 2021). Respon anggrek Dendrobium sp., Oncidium sp., dan Phalaenopsis sp. terhadap pemberian empat jenis nutrisi organik yang berbeda pada tahap regenerasi planlet. Agrikultura. 32(1): 27–36.

Andaryani S. 2010). Kajian penggunaan berbagai konsentrasi BAP dan 2,4-D terhadap induksi kalus jarak pagar (Jatropha curcas L.) secara In vitro. [skripsi]. Fakultas Pertanian, UNS, Solo.

Ariyanti N, Erawati D, Sarita R, Belinda S. 2021. Analisis peran air kelapa terhadap pertumbuhan eksplan kultur Vanili (Vanilla planifolia). Agropross. National Conference Proceedings of Agriculture, 5.

Atmaja K, Tika I, Wijaya I. 2017. Pengaruh perbandingan komposisi bahan baku terhadap kualitas kompos dan lama waktu pengomposan. Biosistem Dan Teknik Pertanian. 5(1): 111–119.

BBPTPH. 2021. Anggrek Dendrobium. Https://Bbtphbanyumas.Distanbun.Jatengprov.Go.Id/Anggrek-Dendrobium?Utm_source=chatgpt.Com. Diakses pada tanggal 7 Januari 2026

Dewi L, Nurcahyani E, Zulkifli, Lande M. 2021). Efek pemberian ekstrak tomat (Solanum lycopersicum L.) terhadap kandungan karbohidrat dan pertumbuhan planlet anggrek Dendrobium Striaenopsis. Agritrop: Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science). 19(1): 67–73.

Edi S. 2014. Pengaruh pemberian pupuk organik terhadap pertumbuhan dan hasil tanaman kangkung darat (Ipomea reptans poir). Jurnal Bioplantae. 3(1): 17–24.

Elfiani, Jakoni. 2015. Sterilisasi eksplan dan sub kultur anggrek, sirih merah dan krisan pada perbanyakan tanaman secara in vitro. Jurnal Dinamika Pertanian. 30(2): 117–124.

Febryanti N, Defiani M, Astarini I. 2017. Induksi pertumbuhan tunas dari eksplan anggrek (Dendrobium heterocarpum Lindl.) dengan pemberian hormon zeatin dan NAA. Metamorfosa: Journal of Biological Sciences. 4(1). 41–47.

Gardner F. 1991. Fisiologi Tanaman Budidaya. Jakarta: Gramedia.

George E, Hall M. 2007. Plant Propagation by Tissue Culture. Springer Netherlands. https://doi.org/10.1007/978-1-4020-5005-3

Hartati S, Nandariyah N, Muliawati E, Sukaya S, Yuniastuti E, Parjanto P, Manurung I. 2016. Karakterisasi morfologi tetua dan hybrid anggrek Dendrobium bigibbum dan Dendrobium lineale. Agrosains: Jurnal Penelitian Agronomi. 24(2): 124–129.

Herliana O, Rokhminarsi E, Iqbal A, Kartini. 2019. Pelatihan pembibitan anggrek secara vegetatif, generatif dan kultur jaringan pada Paguyuban Mantan Buruh Migran “Seruni” Kabupaten Banyumas. Jurnal Ilmiah Pengabdian Kepada Masyarakat. 3(2): 61–69.

Impitasari N, Nurcahyani E, Handayani T, Yulianty Y. 2019. Pertumbuhan planlet krisan (Dendranthema grandiflora Tzvelev) kultivar Pink Fiji setelah penambahan ekstrak tauge (Vigna radiate L.) pada medium Murashige and Skoog (MS) secara in vitro. Jurnal Ilmiah Biologi Eksperimen Dan Keanekaragaman Hayati (J-Bekh). 5(2). 36–41.

Intias S. 2011. Pengaruh pemberian berbagai konsentrasi 2,4-D dan BAP terhadap pembentukan kalus purwoceng (Pimpinella pruatjan) secara in-vitro. 1(1): 1–10.

Kasutjianingsih R, Poerwanto N, Khumaida, Efendi D. 2010. Kemampuan pecah tunas dan kemampuan berbiak mother plant pisang rajabulu (AAB) dan pisang tanduk (AAB) dalam medium inisiasi in vitro. Agriplus. 20(1): 1–9.

Kindlmann P, Kull T, McCormick M. 2023. The distribution and diversity of orchids. Diversity. 15(7): 810. DOI: https://doi.org/10.3390/d15070810

Marlina G, Marlinda M, Rosneti H. 2019. Uji penggunaan berbagai media tumbuh dan pemberian pupuk growmore pada aklimatisasi tanaman anggrek Dendrobium. Jurnal Ilmiah Pertanian. 15(2): 105–114.

Naive MAK, Yudistira YR, Victoriano M, Ormerod P. 2021. Amended description of the Sumatran endemic Dendrobium bandii (Orchidaceae) with notes on its conservation status and ecology. Lankesteriana. 21(1). DOI: https://doi.org/10.15517/lank.v21i1.45856

POWO (Plants of the World Online). 2025. Dendrobium Sw. Plants of the World Online. Https://Powo.Science.Kew.Org/Taxon/Urn:Lsid:Ipni.Org:Names:325886-2. Diakses pada 7 Januari 2026

Purnamasari A, Ratnawati R, Suyitno A, Sugiyarto L, Mercuriani I. 2020. Optimasi Media kultur in vitro anggrek Dendrobium nobile berbasis pupuk dengan penambahan air kelapa dan Vitamin B1. Jurnal Penelitian Saintek. 25(2): 157–172.

Rugayah, Suherni D, Cahya Ginting Y, Karyanto A. 2021. The effect of shallot and tomato extract consentrations on the growth of mangosteen seedling (Garcinia mangostana L.). Jurnal Hortikultura Indonesia. 12(1): 42–50. DOI: https://doi.org/10.29244/jhi.12.1.42-50

Saepudin A, Yulianto Y, Aeni R. 2020. Pertumbuhan eksplan in vitro anggrek hibrida Dendrobium pada beberapa media dasar dan konsentrasi air kelapa. Jurnal Pertanian. 5(2): 97–115.

Sallolo S, Sadimantara G, Wijayanto T. 2012. Pertumbuhan anggrek Dendrobium candy streep lasiantera pada media Sapih Vacint and Went secara in vitro dengan penambahan ekstrak pisang raja dan fish emulsion. Jurnal Berkala Penelitian Agronomi. 1(1): 57–62.

Setiawati T, Nurzaman M, Rosmiati E, Pitaloka G. 2016. Pertumbuhan tunas anggrek Dendrobium Sp. menggunakan kombinasi benzyl amino purin (BAP) dengan ekstrak bahan organik pada media Vacin and Went (VW). J. Pro-Life. 3(3): 143–152.

Suaib, Sadimantara I. 2014. Kultur Jaringan Tanaman. Sulo Printing.

Suárez J, Cevallos D, Pérez J. 2022. Habitat diversity promotes and structures orchid diversity. Acta Oecologica. 118(103894).

Sulichantini E, Eliyani E, Saputra A, Susylowati S. 2021. Pengaruh zat pengatur tumbuh dan bahan organik terhadap pertumbuhan anggrek tebu Grammatophyllum speciosum Blume. secara kultur jaringan. Jurnal Agroekoteknologi Tropika Lembab. 4(1).

Tuhuteru S, Hehanussa M, Raharjo S. 2012. Pertumbuhan dan perkembangan anggrek Dendrobium anosmum pada media kultur in vitro dengan beberapa konsentrasi air kelapa. Agrologia. 1(1): 1–12.

UGM (Universitas Gadjah Mada). 2023. Preserving orchids: UGM expert highlights need for collaborative conservation. Https://Ugm.Ac.Id/En/News/Preserving-Orchids-Ugm-Expert-Highlights-Need-for-Collaborative-Conservation/. Diakses pada 7 Januari 2026.

Widiastoety D, Solvia N, Soedarjo M. 2010. Potensi anggrek dendrobium dalam meningkatkan variasi dan kualitas anggrek bunga potong. Jurnal Litbang Pertanian. 29(3): 101–106.

Yustisia D, Arsyad M, Wahid A, Jumadil Asri J. 2018. Pengaruh pemberian zpt alami (air kelapa) pada media MS 0 terhadap pertumbuhan planlet tanaman kentang (Solanum tuberosum L.). Jurnal Agrominansia. 3(2): 130–140.




DOI: https://doi.org/10.20527/b.v23i2.19775

Refbacks

  • There are currently no refbacks.