THE ANTIBACTERIAL EFFECT OF KASTURI LEAF EXTRACT (Mangifera casturi) AGAINST THE GROWTH OF Streptococcus mutans

Naila Khairiyah, Didit Aspriyanto, Deby Kania Tri Putri

Abstract


ABSTRACT

Background: Dental caries is a common problem of teeth and mouth. Dental caries is caused by a complex biological interaction between acidogenic bacteria, fermented carbohydrates and host factors, such as teeth and saliva. Streptococcus mutans is known as one of the main bacteria that causes dental caries. Kasturi leaf extract (Mangifera casturi) has secondary metabolite compounds, such as alkaloids, terpenoids, flavonoids, phenolics and saponins which are efficacious as antibacterial. Objective: The aim of this study was to analyzing the effectiveness of antibacterial kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus mutans bacteria. Method: This study was true experimental research with randomized pretest-posttest with control group design with 8 treatment groups consisted of kasturi leaves extract (concentration with 20 mg/ml, 25 mg/ml, 30 mg/ml, 35 mg/ml,  40 mg/ml and 45 mg/ml), positive control (chlorhexidine 0.2%) and negative control (aquades). Each treatment was repeated three times. Testing was done by liquid dilution method to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The data were analyzed using One Way Anova 95% (α=0.05) followed by Post hoc LSD for MIC data and Dunnet T3 for MBC data. Results: Based on the results of the analysis, it was known that Kasturi leaf extract had antibacterial effectiveness against the growth of Streptococcus mutans bacteria with MIC obtained at a concentration of 20 mg/ml and MBC obtained at 40 mg/ml. Conclusion: Kasturi leaf extract has an antibacterial effect against the growth of Streptococcus mutans.

 

Key words: Antibacterial, kasturi leaf extract (Mangifera casturi), MIC, MBC, Streptococcus mutans

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References


Veiga, Nelio. Dental Caries: A Review. Journal of Dental and Oral Health. 2016 ; 2 (5) : 1-5

Forssten, Sofia D; Björklund, Marika; Ouwehand, Arthur C. Streptococcus mutans, Caries and Simulation Models. Nutrients Journal. 2010 ; 2 (1) : 290-298

Riwandy, Achmad ; Aspriyanto, Didit ; Budiarti, Lia Yulia. Aktivitas Antibakteri Ekstrak Air Kelopak Bunga Rosella (Hibiscus sabdariffa L) Terhadap Pertumbuhan Streptococcus mutans In Vitro. Dentino Jurnal Kdokteran Gigi. 2014 ; 2 (1) : 60-64

Arif, Nurul Afriani; Sukmawaty, Eka; Masri, Mashuri. Sumber Daya Alternatif Antimikroba Terhadap Bakteri Streptococcus mutans Sebagai Dental Caries (Sebuah Review). Prosiding Seminar Nasional Biology for Life. 2017 ; 1(1) : 44-48

Fajriani; N, Jennifer; Andriani. Reduction of Salivary Streptococcus mutans Colonies in Children After Rinsing with 2.5% Green Tea Solution. Journal of Dentistry Indonesia. 2014 ; 21 (3) : 79-84

Rosdiana, Nova ; Nasution, Abdillah Imron. Gambaran Daya Hambat Minyak Kelapa Murni dan Minyak Kayu Putih Dalam Menghambat Pertumbuhan Streptococcus mutans. J Syiah Kuala Dent Soc. 2016 ; 1 (1) : 43–50

Rahim, M. Andry; Suartha, I Nyoman; Sudimartini, Luh Made. Efek Imunostimulator Ekstrak Daun Kasturi (Mangifera Casturi) Pada Mencit. Indonesia Medicus Veterinus. 2017 ; 6 (1) : 10-19

Juliantina, F. R. Manfaat Sirih Merah (Piper crocatum) Sebagai Agen Antibakteri Terhadap Bakteri Gram Positif dan Gram Negatif. Journal Kedokteran dan Kesehatan Indonesia (JKKI). 2009 ; 1 (1) : 345

Marliani, Lia; Naimah, Anni; Roni, Asep. Penetapan Kadar Fenolat Total dan Flavonoid Total Ekstrak Etanol Daun, Kulit Batang dan Kulit Buah Kasturi (Mangifera Casturi ). Prosiding Seminar Nasional Tumbuhan Obat Indonesia Ke-50. 2016 ; 1 (1) : 1-8

Rachmawati, F dan Nuria, C M. Uji Aktivitas Antibakteri Fraksi Kloroform Ekstrak Etanol Pegagan (Centella asiatica) Serta identifikasi Senyawa Aktifnya. Jurnal Fakultas Farmasi Universitas Hasyim Semarang. 2011 ; 1(1) : 1-13

Rosyidah, K ; Nurmuhaimina, S A ; Komari, N ; Astuti, M D. Aktivitas Antibakteri Fraksi Saponin Dari Kulit Batang Tumbuhan Kasturi (Mangifera casturi). Al-Chemy Jurnal. 2010 ; 1 (2) ; 65-69

Putri, R ; Mursiti, S ; Sumarni, W. Aktivitas Antibakteri Kombinasi Temu Putih dan Temulawak terhadap Streptococcus mutans. Jurnal MIPA. 2017 ; 40 (1) : 43-47

Yuliana ; Ilyas, Asriani ; Suriani. Isolasi Senyawa Bioaktif Antibakteri Pada Ekstrak Etanol Teripang Pasir (Holothuri scabra) di Kepulauan Selayar. Al-Kimia Journal. 2017 ; 5(1) : 71-80

Suwito, Marani Bafianti ; Wahyunitisari, Manik Retno ; Umijati, Sri. Efektivitas Ekstrak Seledri (Apium graveolens L. var. secalinum Alef.) Terhadap Pertumbuhan Bakteri Streptococcus mutans Sebagai Alternatif Obat Kumur. Jurnal Kedokteran Syiah Kuala. 2017 ; 17 (3) : 159-163

Azzahra, Fadhillah ; Hayati, Maulida. Uji Aktivitas Ekstrak Daun Pegangan (Centella asiatica (L). Urb) Terhadap Pertumbuhan Streptococcus mutans. Jurnal B-Dent. 2018 ; 5 (1) : 9-19

Nimah, Shopiatun. Uji Bioaktivitas Ekstrak Teripang Pasir (Holothuri scabra) Terhadap Bakteri Pseudomonas aeruginosa dan Bacillus cereus. Jurnal Perikanan. 2012 ; 1 (1) : 1-9

Sinaredi, Betadion Rizki ; Pradopo, Seno ; Wibowo, Teguh Budi. Daya antibakteri obat kumur chlorhexidine, povidone iodine, fluoride suplementasi zinc terhadap, Streptococcus mutans dan Porphyromonas gingivalis. Dent. J. (Maj. Ked. Gigi). 2014 ; 47 (4) : 211–214

Dewi MK, Ratnasari E, Trimulyono G. Aktivitas Antibakteri Ekstrak Daun Majapahit (Crescentia cujete) Terhadap Pertumbuhan Bakteri Ralstonia solanacearum Penyebab Penyakit Layu. LentaBio. 2014 ; 3 (1) : 51-57.

Winarti ; Kusrini, Dewi ; Fachriyah, Enny. Isolasi, Identifikasi dan Uji Aktivitas Antibakteri Minyak Atsiri Akar Sidaguri (Sida rhombifolia Linn). Jurnal Kimia Sains dan Aplikasi. 2009 ; 12 (2) : 52-56

Bowen, W H ; Koo, H. Biology of Streptococcus mutans – Derived Glucosyltransferases : Role in Extracelluler Matrix Formation of Cariogenic Biofilm. Caries Res Journal. 2011 ; 45 (1) : 69-86

Carmona, M ; Heath, T ; Oc, T ; Tiesdell, S. Public Space – Urban Space, The Dimention of Urban Design. Edisi 2. Oxpord : Elsevier ; 2010. p. 114

Syarifuddin, Nur Indriyani; Badruzsaufari; Ni’mah, Malikhatun. Perbandingan Daya Hambat Antibakteri Ekstrak Kulit Batang Kasturi (Mangifera casturi Kosterm) dengan Ekstrak Daun Sirih (Piper betle L.) Terhadap Pertumbuhan Streptococcus mutans 2302-Unr Secara In Vitro. Jurnal Pharmascience. 2014 ; 1 (2) : 46-53

Rinawati ND. Daya Antibakteri Tumbuhan Majapahit (Crescentia cujete L.) Terhadap Bakteri Vibrio alginolycticus. Surabaya: Jurusan Biologi Fakultas Matematika Ilmu Pengetahuan Alam Institus Teknologi Sepuluh November. 2010 ; 1 (1) : 1-5




DOI: https://doi.org/10.20527/dentin.v3i3.1344

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