EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN KASTURI (Mangifera casturi) TERHADAP PERTUMBUHAN BAKTERI Aggregatibacter actinomycetemcomitans

Luthfi Ridhwana, Fransiska Uli Arta Puspitasari, Yusrinie Wasiaturrahmah

Abstract


ABSTRACT

Background: Prevalence of periodontitis in Indonesia is 74.1%, and periodontitis type that causes mayor periodontal damage with minimal microbiological factors is Aggressive Periodontitis. Microbiological factor in aggressive periodontitis is Aggregatibacter actinomycetemcomitans which producing toxins which stimulate the immune response and tissue damage. Many researchers today discover new antibiotic ingredients with a minimal side effect such as Kasturi plant. Kasturi leaf extract contains triterpenoids, flavonoids, tannins, and phenolic, which have the antibacterial effect. Objective: To Identifying and analyzing the effectiveness of antibacterial effect on Kasturi leaves extract against Aggregatibacter Actinomcetemcomitans growth. Methods: This study used true experimental post-test only with control group design by broth and agar dilution method to determine Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The result data analyzed with Kruskal-Wallis and Mann Whitney Post hoc test. Results: MIC was found at 20 mg/ml concentration and MBC at 40 mg/ml concentration. Conclusion: Kasturi leaf extract was effective in inhibited and stopped Aggregatibacter actinomycetemcomitans growth.

Keywords :  Aggregatibacter actinomycetemcomitans, Aggressive Periodontitis, Kasturi leaf extract (Mangifera casturi), MBC, MIC.

ABSTRAK

Latar Belakang: Prevalensi periodontitis di Indonesia sebesar 74,1% dan periodontitis yang menimbulkan kerusakan besar dengan faktor mikrobiologi yang minimal yaitu periodontitis agresif. Faktor mikrobiologi berupa bakteri Aggregatibacter actinomycetemcomitans yang memproduksi toksin penyebab gangguan respons imun dan kerusakan jaringan. Penelitian mengenai bahan antibiotik dengan efek samping minimal telah banyak dilakukan salah satunya dengan tumbuhan Kasturi. Daun Kasturi mengandung senyawa berupa triterpenoid, flavonoid, tanin, dan fenol yang bersifat antibakteri. Tujuan: Mengetahui dan menganalisis efektivitas antibakteri ekstrak daun kasturi terhadap pertumbuhan bakteri Aggregatibacter actinomycetemcomitans. Metode: True experimental dengan desain post test only with control group dan mempunyai 6 kelompok perlakuan dengan 6 kali pengulangan. Penelitian ini menggunakan metode dilusi cair dan padat untuk mengetahui nilai Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM). Analisis data menggunakan metode Kruskal Wallis dan uji Post hoc Mann Whitney. Hasil: KHM pada ekstrak daun kasturi terdapat pada konsentrasi 20 mg/ml dan KBM pada konsentrasi 40 mg/ml. Kesimpulan: Ekstrak daun kasturi efektif dalam menghambat dan menghentikan pertumbuhan bakteri Aggregatibacter actinomycetemcomitans.

Kata kunci :  Aggregatibacter actinomycetemcomitans, Ekstak Daun Kasturi (Mangifera casturi), KBM, KHM, Periodontitis Agresif,


Full Text:

PDF

References


Newman MG, Takei HH, Klokkevold PR, Carranza FA. Newman and Carranza’s Clinical Periodontolgy 13th Edition. Canada: Elsevier Saunders; 2019. p. 1917-1943; 2665-3098.

Lang P, Lindhe J. Clinical Periodontology and Implant Dentistry 6th Ed. UK: John Willey & Sons Ltd; 2015. p. 391-434.

Dorothy PA, Beemsterboer PL, Essex G. Periodontology For The Dental Hygienis. 4th Edition. China: Elsevier Saunders; 2014. p. 97; 223-226.

Taconelli E, Carrara E, Savoldi A, Harbath S, Mendelson M, Monnet DL, Pulcini C, Kahlmeter G, Kluytmans J, Carmeli Y, Outterson K, Patel J, Cavaleri M, Cox EM, Houchens C, Grayson ML, Hansen P, Singh N, Theuretzbacher U, Magrini N. Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis. Lancet Infect Dis. 2017; 17: 1-10.

Rosyidah K, Nurmuhaimina SA, Komari N, Astuti MD. Aktivitas Antibakteri Fraksi Saponin dari Kulit Batang Tumbuhan Kasturi (Mangifera Casturi). Alchemy. 2010; 1(2): 53-103.

Rahim MA, Suartha IN, Sudimartini LM. Efek Immunostimulator Ekstrak Daun Kasturi (Mangifera casturi) Pada Mencit. Indonesia Medicus Veterinus. 2017; 6(1): 10-19.

Nuraini FR, Setyaningsih R, Susilowati A. Antioxidant activity of bioactive compound produced by endophytic fungi isolated from endemic plant of South Kalimantan Mangifera casturi Kosterm. International Conference on Biology and Applied Science. 2019; 2120: 1-9.

Darmawan ARB. Efforts to improve the quality of kasturi mango (Mangifera casturi) with modification of cultivation. Pros Sem Nas Masy Biodiv Indon. 2015; 1(4): 894-899.

Tanaya V, Retnowati R, Suratmo. Fraksi Semi Polar dari Daun Mangga Kasturi (Mangifera casturi Koesterm). Kimia Student Journal. 2015; 1(1): 778-784.

Dahlan MS. Besar Sampel dan Cara Pengambilan Sampel dalam Penelitian Kedokteran dan Kesehatan. Edisi 3. Jakarta: Salemba Medika; 2010. p. 25-76.

Astutiningsih C, Setyani W, Hindratna H. Uji Daya Antibakteri dan Identifikasi Isolat Senyawa Katekin dari Daun Teh (Camellia sinensisL. var Assamica). Jurnal Farmasi Sains dan Komunitas. 2014; 11(2): 50-57.

Afrina, Chismirina S, Aulia CRP. Konsentrasi Hambat dan Bunuh Minimum Ekstrak Buah Kapulaga (Amomum compactum) Terhadap Aggregatibacter actinomycetemcomitans. Jurnal Syiah Kuala Dent Soc. 2016; 1(2): 192-200.

Ardiana, Oktiani BW, Panjaitan FUA. Efek Antibakteri Ekstrak Flavonoid Propolis Kelulut (Geniotrigona thorasica) Terhadap Aggregatibacter Actinomycetemcomitans (Studi In Vitro Melalui Metode Dilusi). Dentin Jurnal Kedokteran Gigi. 2019; 3(2): 48-54

Chung PY, Navaratnam P, Chung LY. Synergistic antimicrobial activity between pentacyclic triterpenoids and antibiotics against Staphylococcus aureus strains. Clinical Microbiology and Antimicrobials. 2011; 10(25): 1-6.

Badal S, Delgoda R. Pharmacognosy Fundamentals, Applications and Strategy. UK: Elsevier Saunders; 2017. p. 199-266.

Christabel PF, Hernando MV, Sutanto CA, Parisihni K. Exploration of Chlorella sp. as Antibacterial to Aggregatibacter Actinomycetemcomitans Biofilm. IOP Conf. Series: Earth and Environmental Science. 2019; 217: 4-5.

Suheri FL, Agus Z, Fitria I. Perbandingan Uji Resistensi Bakteri Staphylococcus aureus Terhadap Obat Antibiotik Ampisilin dan Tetrasiklin. Andalas Dental Journal. 2019: 25-33.

Xie Y, Yang W, Tang F, Chen X, Ren L. Antibacterial Activities of Flavonoids: Structure-Activity Relationship and Mechanism. Current Medicinal Chemostry. 2015; 22: 132-149.

Khairiyah N, Aspriyanto D, Putri DKT. The Antibacterial Effect Of Kasturi Leaf Extract (Mangifera casturi) Against The Growth OF Streptococcus mutans. Dentin Jurnal Kedokteran Gigi. 2019; 3(3): 91-96.

Sari DP, Aspriyanto D, Taufiqurrahman I. Antibacterial Effectivity Of Kasturi Leaf Extract (Mangifera casturi) AGAINST THE Growth Of Streptococcus sanguinis Bacteria. Dentino Jurnal Kedokteran Gigi. 2020; 5(1): 37.

Ayu ND, Indraswary Rm Chtistiono S. Efektivitas Ekstrak Daun Jambu Mete (Anacardium Occidentale L) Terhadap Pertumbuhan Aggregatibacter actinomycetemcomitans Pada Gingivitis - In Vitro. ODONTO. 2014; 1(1): 44-46.

Sieniawska E, Baj T. Pharmacognosy. USA: Elsevier Saunders; 2017. p. 199-227.

Amir HM, Duarte CMM, Maiza F. Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens. Industrial Crops and Product. 2015; 67: 249-256.

Muljono P, Fatimawali, Manampiring AE. Uji aktivitas antibakteri ekstrak daun mayana jantan (Coleus atropurpureus Benth) terhadap pertumbuhan bakteri Streptococcus Sp. dan Pseudomonas Sp. Jurnal e-Biomedik (eBm). 2016; 4(1): 170.

Khotimah H, Anggraeni EW, Setianingsih A. Karakterisasi Hasil Pengolahan Air Menggunakan Alat Destilasi Characterization Of Water Processing Using Distilation Equipment. Jurnal Chemurgy. 2017 Desember; 1(2): 34.




DOI: https://doi.org/10.20527/dentin.v4i2.2285

Refbacks

  • There are currently no refbacks.


Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia
E-mail. [email protected]

Website. fkg.ulm.ac.id