AKTIVITAS ANTIBAKTERI EKSTRAK DAUN RAMBAI (Sonneratia Caseolaris) KONSENTRASI 70%, 80% DAN 90% TERHADAP Streptococcus Mutans IN VITRO

Helda Helda, Didit Aspriyanto, R. Harry Dharmawan S

Abstract


ABSTRACT

Background:. One of the factors causing dental caries is a microorganism, namely Streptococcus mutans. Rambai leaf extract (Sonneratia caseolaris) contain triterpenoids, flavonoids, tannins, steroids and phenols which have antibacterial activity against the growth of Streptococcus mutans which has the potential to prevent dental caries. Purpose: This study aims to prove the presence of 70%, 80% and 90% antibacterial activity of rambai leaf extract (Sonneratia caseolaris) in inhibiting the growth of Streptococcus mutans in vitro. Methods: This study was a laboratory experimental study, with a post test only design, using a completely randomized design consisting of 5 treatment groups, namely: rambai leaf extract 70%, 80% and 90% (Sonneratia caseolaris), positive control. (Povidone iodine 1%) and negative control (distilled water) were repeated 5 times. Antibacterial activity was assessed from the clear inhibition zone formed on Muller Hinton media by diffusion method. Results: The Kruskal-wallis test showed that the clear inhibition zone formed had a significant difference. The average clear zone of rambai leaf extract (Sonneratia caseolaris) was 70% at 11.6 mm, 80% for 12.9 mm and 90% for 14.2 mm. Conclusion: Rambai leaf extract (Sonneratia caseolaris 70%, 80% and 90% have antibacterial activity in inhibiting the growth of Streptococcus mutans in vitro.

 

Keywords: Antibacterial Activities, Rambai Leaf Extract, Streptococcus mutans.

 

ABSTRAK

Latar Belakang: Faktor penyebab terjadinya karies gigi salah satunya adalah mikroorganisme yaitu Streptococcus mutans. Ekstrak daun rambai (Sonneratia caseolaris) mengandung triterpenoid, flavonoid, tanin, streoid dan fenol yang  memiliki aktivitas antibakteri terhadap pertumbuhan Streptococcus mutans yang berpotensi sebagai pencegah karies gigi. Tujuan: Tujuan penelitian ini adalah untuk membuktikan adanya aktivitas antibakteri ekstrak daun rambai (Sonneratia caseolaris) 70%, 80% dan 90% dalam menghambat pertumbuhan Streptococcus mutans in vitro. Metode: Penelitian ini merupakan penelitian eksperimental laboratorik murni (true experimental), dengan post-test only design, menggunakan rancangan acak lengkap terdiri dari 5 kelompok perlakuan, antara lain: ekstrak daun rambai (Sonneratia caseolaris) 70%, 80% dan 90%, kontrol positif (Povidone iodine 1%) dan kontrol negatif (akuades) dilakukan pengulangan sebanyak 5 kali. Aktivitas antibakteri dinilai dari zona hambat bening yang terbentuk pada media Muller Hinton dengan metode difusi. Hasil: Uji Kruskal-wallis menunjukkan bahwa zona hambat bening yang terbentuk memiliki perbedaan yang bermakna. Rata-rata zona bening ekstrak daun rambai (Sonneratia caseolaris) yaitu 70% sebesar 11,6 mm, 80% sebesar 12,9 mm dan 90% sebesar 14,2 mm. Kesimpulan: Ekstrak daun rambai (Sonneratia caseolaris) 70%, 80% dan 90% memiliki aktivitas antibakteri dalam menghambat pertumbuhan Streptococcus mutans in vitro.

 

Kata kunci: Aktivitas Antibakteri, Ekstrak Daun Rambai, Streptococcus mutans.

Full Text:

PDF

References


Adhani R, Priyawan R, Widodo, Tutung N. Pengembangan Model Pemberdayaan Masyarakat Lahan Basah untuk Membudayakan Gosok Gigi dengan Air yang Memenuhi Persyaratan Kesehatan dalam Penanggulangan Tingginya Indeks Karies Gigi di Kalimantan Selatan. Final Report of Universitas Lambung Mangkurat. 2015; 1(2); 1-8.

Badan Penelitian dan Pengembangan Kesehatan Kementerian RI. Riset Kesehatan Dasar (Riskesdas 2018). Jakarta: Kementerian Kesehatan RI; 2018.

Bao X, Jingmei Y, J.J. De Soet, Hongyan L, Xuejun G, Cor VL dkk. Factors Influencing the

Competition between Streptococcus oligofermentans and Streptococcus mutans in Dual-Species Biofilms. Caries Res. 2017; 51(1) :507–514.

Mingyun L, Huang R , Zhou X, Qiu W, Xu X, Gregory RL. Effect of nicotine on cariogenic virulence of Streptococcus mutans. Folia Microbiologica. 2016; 61(6): 505–512.

Meyer F, Enax J. Early Childhood Caries: Epidemiology, Aetiology, and Prevention. International Journal of Dentistry. 2018; ID 1415873: 1-7.

San FC, Chien HL, Shu WC. Povidone Iodine Application InducesCcorneal Cell Death Through Fixation. British Journal of Ophtalmology. 2011; 95: 83-227.

Rifdayani N, Lia YB, Amy NC. Perbandingan Efek Bakterisidal Ekstrak Mengkudu (Morinda citrifolia Liin) 100% dan Povidone Iodine 1% Terhadap Streptococcus mutans In Vitro. Dentino (Jur. Ked. Gigi). 2014; 2(1): 1- 6.

Putri S, Aziz D, Rahmatini, Cimi I. Perbandingan Daya Hambat Larutan Antiseptik Povidone iodine dengan Ekstrak Daun Sirih terhadap Candida albicans secara In Vitro. Jurnal Kesehatan Andalas. 2015; 4(3): 962-966.

Mervrayano J, Rahmatini, Elizabeth B. Perbandingan Efektivitas Obat Kumur yang Mengandung Chlorhexidine dengan Povidone Iodine terhadap Streptococcus mutans. Jurnal Kesehatan Andalas. 2015; 4(1): 168-171.

Yulianis M, Latief M, Redho M. Isolasi senyawa dari Fraksi Eil Asetat Daun Pedada (Sonneratia caseolaris L.) dan Uji Aktifitas Antioksidan. Prosiding Seminar Nasional dan Workshop “Perkembangan Sains Farmasin dan Klinik 5”, 2015; 1(1); 1-90.

Herika, Rita K, Triana UP, Indriyati. Skrining Fitokimia dan Uji Toksisitas Akut Ekstrak Daun Berembang (Sonneratia caseolaris) Sebagai Antihipertensi. Jurnal Jeumpa. 2018; 5(2): 126131.

Sogandi, Frensiska Anggelia, Lilih Riniwasih K. Antibacterial Activity Test of 96% Ethanol Extract of Rambai Leaf (Sonneratia caseolaris, (L.) Engl) Against Escherichia coli Bacterium. Indonesia Natural Research Pharmaceutical Journal. 2017; 2(1): 73-80.

Ahmad I, Neneng SSA, Arif L, Muhammad AMM, Laode R, Abdul M. In vitro Antimicrobial Activity Evaluation of Mangrove Fruit (Sonneratia caseolaris L.) Extract. Pharmacogn J. 2018; 10(3): 598-601.

Wijaya H, Novitasari, Siti J. Perbandingan Metode Ekstraksi Terhadap Rendeman Ekstrak Daun Rambai Laut (Sonneratia caseolaris L. Engl). Jurnal Ilmiah Manuntung. 2018; 4(1): 79-83.

Lestari RD, R Susanti. Effectivity of Pedada Fruit (Sonneratia caseolaris) Extract to The Level of Sgot and Sgpt in Rat Treated by Paracetamol Induction. Biosaintifika Journal of Biology & Biology Education. 2016; 7(1): 29-36.

Lutfiasari N, Sri A, Aulia A. The Effect of Giving Infusion of Rambai (Sonneratia caseolaris) Fruit on the Growth of Escherichia coli Bacteria in Vitro. Prosiding Seminar Nasional Lahan Basah Tahun 2016 Jilid 1: 255-259.

Alfian NM, Maharani LA. Efektivitas antibakteri ekstrak metanol batang pisang Mauli (Musa acuminata) dan povidone iodine 10% terhadap Streptococc us mutans. Jurnal PDGI. 2014; 63(3): 78-83.




DOI: https://doi.org/10.20527/dentin.v4i3.2595

Refbacks

  • There are currently no refbacks.


Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia
E-mail. [email protected]

Website. fkg.ulm.ac.id