PENGARUH PERENDAMAN LARUTAN KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP PELEPASAN KALSIUM PADA GIGI
Abstract
ABSTRACT
Background: Apart from being antibacterial, chitosan (Channa striata) fish scales solution can also be used to maintain tooth structure by reducing the solubility rate of hydroxyapatite or preventing demineralization of teeth in acidic conditions. Objective: To examine the effect of immersion of chitosan solution of scaled fish (Channa striata) 2,5% and 5% on the release of calcium (Ca) teeth. Method: Experimental study with a post-test only with control group design consisting of 3 treatment groups, namely negative control, a concentration of 2,5%, and 5% where each treatment after teeth were immersed in a solution of lactic acid with a pH 5,2 by reading the value of the release of tooth calcium released using atomic absorption spectrophotometry (AAS). Results: The concentrations of 2,5% and 5% were able to inhibit the release of calcium in the teeth. Conclusion: Chitosan scales chitosan (Channa striata) solution at a concentration of 2,5% has the best potential as a biomaterial in inhibiting demineralization.
Keywords: Acid, Calcium, Chitosan, Demineralization, Haruan.
ABSTRAK
Latar Belakang: Larutan kitosan sisik ikan haruan (Channa striata) selain bersifat sebagai antibakteri juga dapat digunakan untuk mempertahankan struktur gigi dengan mengurangi kecepatan kelarutan hidroksiapatit atau mencegah demineralisasi gigi dalam kondisi asam. Tujuan: Menguji pengaruh perendaman larutan kitosan sisik ikan haruan (Channa striata) 2,5% dan 5% terhadap pelepasan kalsium (Ca) gigi. Metode: Metode eksperimental murni dengan post test-only with control group design terdiri dari 3 kelompok perlakuan yaitu kontrol negatif, larutan kitosan sisik ikan haruan konsentrasi 2,5%, dan 5% yang diberikan pada gigi yang direndam pada larutan asam laktat dengan pH 5,2 dengan pembacaan kadar pelepasan kalsium gigi yang terlepas menggunakan alat spektrofotometri serapan atom (SSA). Hasil: Pada konsentrasi 2,5% sudah mampu menghambat pelepasan kalsium pada gigi. Kesimpulan : Larutan kitosan sisik ikan haruan (Channa striata) konsentrasi 2,5% dan 5% berpotensi sebagai biomaterial dalam menghambat demineralisasi pada gigi.
Kata kunci : Asam, Demineralisasi, Haruan, Kalsium, Kitosan.
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Riset Kesehatan Dasar (Riskesdas) 2018. Badan Penelitian dan Pengembangan Kesehatan Kementerian RI. 2018. p.204
Colak H, Dülgergil CT, Dalli M, Hamidi MM. Early childhood caries update: A review of causes, diagnoses, and treatments. J Nat Sci Biol Med. 2013;4(1):29–38.
Ayilliath, Ambili & Savitha, Sathyaprasad & Kottigade, Subbannaya & Sreeshma, & Kamath, Ananth. (2013). Relationship of Severe Early Childhood Caries to Maternal Microbial Flora and Salivary Buffering Capacity. Health Sciences. 2013;2(2).
Panigoro S, Pangemanan DHC, Juliatri. Kadar Kalsium Gigi yang Terlarut pada Perendaman Minuman Isotonik. Jurnal e-GiGi (eG). 2015: 3(2) : 356- 360.
Hidayat S, Adhani R, Arywa IW. Perbedaan pH Saliva Menggosok gigi Sebelum dan Sesudah Mengonsumsi Makanan Manis dan Lengket (Pengukuran Menggunakan pH Meter pada Anak Usia 10-12 Tahun di SDN Melayu 2 Banjarmasin). Jurnal Dentino Kedokteran Gigi. 2014. Vol II. No 1 : 39-45.
Surija I, Gunawan HA, Amir LR. Effect of chitosan on the enamel demineralization process in vitro: an enamel solubility test. Journal of Physics: 2018. Conf. Series 1073 : 1-6.
Faridah F, Khafidzoh A, Mustikawati D, Anggraeni N, Dharmawan Y. Chitosan Pada Sisik Ikan Bandeng (Chanos Chanos) Sebagai Alternatif Pengawet Alami Pada Bakso. Jurnal Ilmiah Mahasiswa. 2012; 2(2):76-79.
Budirahardjo, R. Sisik Ikan Sebagai Bahan yang Berpotensi Mempercepat Proses Penyembuhan Jaringan Lunak Rongga Mulut, Regenerasi Dentin Tulang Alveolar. Stomatognatic (J K G Unej). 2010; 7(2): 136-140.
Widyaningrum DRW, Putri DKT, Taufiqurrahman I. Antibacterial Activities of Chitosan In Haruan Fish Scales (Channa striata) to The Growth of Staphylococcus aureus. Jurnal Dentino Kedokteran Gigi. Vol IV. No 2. September 2019 : 162 - 167
Putri DKT et.al. Synthesis and Characteristics of Chitosan from Haruan (Channa striata) Fish Scales. Sys Rev Pharm. 2020; 11(4): 15 20.
Mahatmanti FW, Sugiyo W, Sunarto W. Sintesis Kitosan dan Pemanfaatannya sebagai Anti Mikroba Ikan Segar. Journal Unnes. 2010. 101-111.
Lussi A, Schlueter N, Rakhmatullina E, Ganss C. Dental Erosion-An Overview with Emphasis on Chemical and Histopathological Aspects. Caries Research. 2011. p. 212
Yuniarti, Achadiyani, Murniati N. Penggunaan Pemutih Gigi Mengandung Hidrogen Peroksida 40% Dibanding dengan Strawberry (Fragaria X ananassa) terhadap Ketebalan Email, Kadar Kalsium, dan Kekuatan Tekan Gigi. Global Medical and Health Communication. 2016. Vol. 4 No. 1: 7-15
Ehrlich H, Koutsoukos PG, Demadis KD, Pokrovsky OS. Principles of Demineralization: Modern Strategies for the Isolation Organic Frameworks. Elsevier. 2009. p. 169-193
Nasution A. Jaringan Keras Gigi Aspek Mikrostruktur dan Aplikasi Riset. Syiah Kuala University Press ; 2016.p.2;p.21;p.31
Featherstone JDB, Lussi A. Understanding the Chemistry of Dental Erosion. Monogr Oral Sci. 2006. 20: 66-76.
Neel EAA, Aljabo A, Strange A, Ibrahim S, Coathup M, Young AM et al. Demineralization-remineralization Dynamics in Teeth and Bone. Dove Press Journal. 2016. 11: 4743-4763.
Visveswaraiah PM, Prasad D, Johnson S. Chitosan A Novel Way to Intervene in Enamel Demineralization - An in Vitro Study. Int J Curr Microbiol Appl Sci. 2014; 3(11): 617–627.
DOI: https://doi.org/10.20527/dentin.v4i3.2599
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