PENGARUH APLIKASI BONDING ANTIBAKTERI TERHADAP JUMLAH BAKTERI LACTOBACILLUS ACIDOPHILUS YANG MELEKAT PADA TUMPATAN RESIN KOMPOSIT BIOAKTIF

Muhammad Muamar Khadafi, Muhammad Yanuar Ichrom Nahzi, Diana Wibowo

Abstract


ABSTRACT

Background: Adhesion of Lactobacillus acidophilus can cause secondary caries and failure of tooth restorations. One of the secondary caries prevention methods is the use of bioactive composite resins which have antibacterial properties. The antibacterial effect is further enhanced by the use of bonding which has antibacterial monomers. The two-step adhesive system in the 6th generation bonding has acid monomers which have been shown to significantly inhibit bacterial growth. Objective: To determine and analyze the effect of bonding application with antibacterial content on the amount of attachment of Lactobacillus acidophilus to bioactive composite resin restoration. Methods: This study design used true experimental with a post-test only design with control group design using 39 premolars 1 tooth divided into 3 groups: the treatment group that was given bonding and the control group that did not use bonding. Results: The results of the One Way ANOVA showed a significant difference (p = 0.000). Post hoc Bonferroni test results showed a significant difference (p = 0.000) between the treatment group and the control group. Conclusion: In the use of antibacterial, non-antibacterial bonding, and without bonding, there were significant differences in the number of attachments of Lactobacillus acidophilus to the restoration material.


Keywords: Antibacterial, Bioactive Composite Resin, Lactobacillus acidophilus.


ABSTRAK

Latar Belakang: Perlekatan Lactobacillus acidophilus dapat menyebabkan terjadinya karies sekunder dan kegagalan restorasi gigi. Salah satu metode pencegahan karies sekunder adalah dengan penggunaan resin komposit bioaktif yang memiliki sifat antibakteri. Efek antibakteri semakin ditingkatkan dengan penggunaan bonding yang memiliki monomer antibakteri. Sistem adhesive  two step pada bonding generasi ke-6 memiliki monomer asam yang terbukti signifikan menghambat pertumbuhan bakteri. Tujuan: Mengetahui dan menganalisis pengaruh aplikasi bonding dengan kandungan antibakteri terhadap jumlah perlekatan bakteri Lactobacillus acidophilus pada material restorasi resin komposit bioaktif. Metode: Desain penelitian ini menggunkan penelitian eksperimental murni (true experimental) dengan rancangan post test only with control group design  menggunakan 39 gigi premolar 1 dibagi menjadi 3 kelompok: kelompok perlakuan yang diberi bonding dan kelompok kontrol yang tidak menggunakan bonding. Hasil: Hasil uji statistik One Way Anova menunjukkan perbedaan bermakna (p = 0,000). Hasil uji Post hoc bonferoni  menunjukkan perbedaan bermakna (p = 0,000) antara kelompok perlakuan dan kelompok kontrol. Kesimpulan: Pada penggunaan bonding antibakteri, bonding non-antibakteri, dan tanpa bonding terdapat perbedaan yang signifikan pada jumlah bakteri Lactobacillus acidophilus yang melekat pada material restorasi.


Kata kunci : Antibakteri, Lactobacillus acidophilus, Resin Komposit Bioaktif.

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References


Sutono E, Rovani CA, Mattulada IK. When Shrinkage is a Problem, This Restoration Can be A Choice: a Case Report. Makassar Dent J. 2017; 6(2): 45-49.

Ramayanti S, Purnakarya I. Peran Makanan Terhadap Karies Gigi. Jurnal Kesehatan Masyarakat. 2013; 7(2): 89-93.

Cheng L, et al. Developing a New Generation of Antimicrobial and Bioactive Dental Resins. Journal of Dental Research. 2017; 96(8): 855-863.

Zhang, et al. Bioactive dental composites and bonding agents having remineralizing and antibacterial characteristics. Dent Clin North Am. 2017; 61(4): 669–687.

Tarle Z, Par M. Bioactive Dental Composite Materials. Journal Medical Sciences. 2018; 45(1): 84-85.

Shafiei F, Memarpour M, Vafamand N, Mohammadi M. Effect of antibacterial/adhesive Approaches on Bonding Durability of Fiber Posts Cemented With Self-etch Resin Cement. Journal section: Operative Dentistry and Endodontics. 2017; 9(9): 1097-1098.

Zettira NZ , Niken P, Pujiana EL. Perlekatan Streptococcus mutans pada Aplikasi Fissure Sealant Berbahan Resin Dibandingkan dengan Ionomer Kaca Fuji VII. Fakultas Kedokteran Gigi Universitas Jember. e-Jurnal Pustaka Kesehatan, 2017; 5 (3): 441-447.

Puspitasari D. Perbandingan Kuat Rekat Resin Komposit pada Dentin dengan Sistem Adhesif Self Etch 1 Tahap dan 2 Tahap (Two Step). Jurnal Kedokteran Gigi Dentino. 2014; 2 (1): 89-94.

Lukomska-Szymanska M, et al. Antibacterial Activity of Commercial Dentine Bonding Systems against E. faecalis–Flow Cytometry Study. Journal MDPI. 2017; 10(1): 7-8.

Ozel E, Kolayli F, Tuna EB, Er D. In Vitro Antibacterial Activity of Various Adhesive Materials Against Oral Streptococci. Biotechnology & Biotechnological Equipment. 2016; 30(1): 122-124.

Korkmaz Y, et al. Microleakage and scanning electron microscopy evaluation of all-in-one selfetch adhesives and their respective nanocomposites prepared by erbium:yttrium-aluminum-garnet laser and bur. Lasers Med Sci. 2010; 25: 493-502.

Imazato S, Chen J, Ma S, Izutani N, Li F. Antibacterial resin monomers based on quaternary ammonium and their benefits in restorative dentistry. Japanese Dental Science Review. 2012; 48: 115-125.

Fujimura Y, Weerasinghe D, Kawashima M. Development Of An Antibacterial Bioactive Dental Adhesive: Simplicity and Innovation. American Journal of Dentistry. 2018; 31: 13-15.

Giammanco MG, et al. In vitro evaluation of the antibacterial activity of cured dentin/enamel adhesive incorporating the antimicrobial agent MDPB. New Microbiol. 2009; (35): 385-390.

Qiu W, Zhou Y, Li Z, Huang T. Application of Antibiotics/Antimicrobial Agents on Dental Caries. BioMed Research International. 2020; 28: 1-11.

Pradiptama Y, Purwanta M, Notopuro H. Antibacterial Effects of Fluoride in Streptococcus mutans Growth in Vitro. Biomolecular and Health Science Journal. 2019; 2(1): 1-3.

Kaushik M, Yadav M. Marginal Microleakage Properties of Activa Bioactive Restorative and Nanohybrid Composite Resin Using Two Different Adhesive In Non Carious Cervical Lesions – An In Vitro Study. West African College of Surgeons. 2017; 7(12): 1-14.

Kim SR, Shin DH. Antibacterial effect of Self-etching adhesive systems on S. Mutans. Restor Dent Endod. 2013:35-38.

Chatzistavrou X, et al. Bactercidal and Bioactive Dental Composite. Front Physiol. 2018; 9: 103.




DOI: https://doi.org/10.20527/dentin.v5i1.3227

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