AKTIVITAS ANTIBAKTERI HIDROGEL MADU KELULUT (Heterotrigona itama) TERHADAP Pseudomonas aeruginosa ATCC 10145

Abstract

Abstract: Bacterial infection is a health problem that often occurs in patients with Diabetes Mellitus and causes diabetic foot ulcers. P. aeruginosa be a pathogen in the wound. For treatment can use natural ingredients kelulut honey. The purpose of this study was to determine whether there was a different antibacterial activity of kelulut honey hydrogel (Heterotrigona itama) concentrations of 20% and 25%, control treatment against P. aeruginosa ATCC 10145 based on diameter of inhibition zone. This study used true experiments with posttests only with control group design. The research data were tabulated, and One-Way ANOVA and Post hoc Duncan tests were carried out until significant differences were found p <0.05. The 20% concentration treatment produced an average inhibition zone diameter of 13.017 mm and a 25% concentration of 17.033 mm. The results showed that there was a 25% concentration hydrogel that has stronger antibacterial activity than other treatments.

Keywords: antibacterial, hydrogel, stingless bee honey, Heterotrigona itama, Pseudomonas aeruginosa

Abstrak: Infeksi bakteri merupakan masalah kesehatan yang sering terjadi pada penderita Diabetes Melitus dan menyebabkan terjadinya luka kaki diabetes. P. aeruginosa menjadi patogen pada luka. Pengobatannya dapat menggunakan bahan alami madu kelulut. Penelitian ini bertujuan untuk mengetahui apakah terdapat perbedaan aktivitas antibakteri hidrogel madu kelulut (Heterotrigona itama) konsentrasi 20% dan 25%, perlakuan kontrol terhadap P. aeruginosa ATCC 10145 berdasarkan diameter zona hambat. Penelitian ini menggunakan metode true experimental dan rancangan posttest only with control group design. Data hasil penelitian ditabulasi kemudian di uji One-Way ANOVA dan Post hoc Duncan hingga ditemukan perbedaan secara nyata dengan p <0,05. Perlakuan konsentrasi 20% menghasilkan rerata diameter zona hambat 13,017 mm dan konsentrasi 25% sebesar 17,033 mm. Hasil penelitian ini menunjukkan konsentrasi 25% memiliki aktivitas antibakteri yang lebih kuat dibandingkan perlakuan lain.

Kata-kata kunci:  antibakteri; hidrogel; madu kelulut; Heterotrigona itama; Pseudomonas aeruginosa