Perbedaan Kadar Kalsium Femur Tikus Setelah Paparan Akut Dan Subkronik Debu Batubara
Abstract
Abstract: Coal dust is a byproduct of coal processing and pollutants in coal mining area and transportation as well. After inhalation of coal dust, the particles will settle in alveoli of the lungs and spread through the vascular system by inflammatory process. The particles contain various minerals that increased substitution or incorporation process of bone mineral in hydroxyapatite lattice, include calcium. The aimed of this research was to determine the difference of Ca2+ levels in femur wistar rats caused by acute and subchronic coal dust exposure. This research was an observational analytic research. Subjects were male wistar rat bone samples taken from Bank Jaringan PEROSI Banjarmasin. Research subjects divided into seven groups: control, acute exposure (14 days) and subchronic exposure (28 days) each with an exposure dose at 6.25 mg/m3, 12.5 mg/m3, and 25 mg/m3. There were four samples femur in each group. Data were analyzed using Kruskal-Wallis test. The result did not show any significant differences of calcium levels in femoral bone wistar rats after acute (p=0,739) and subchronic exposure (p= 0,123). The conclusion, there were no significant differences of calcium levels in femur rats after exposure to coal dust with various dosage.
Keywords: Calcium levels, coal dust, wistar rats, acute, subchronic.
ABSTRAK: Debu batubara merupakan produk samping pengolahan batubara sekaligus polutan di area pertambangan dan transportasi batubara. Partikel tersebut mengandung berbagai macam mineral yang dapat memicu proses substitusi atau inkorporasi mineral tulang dalam kristal hidroksiapatit, salah satunya mineral kalsium. Penelitian bertujuan untuk mengetahui perbedaan kadar Ca2+ femur tikus wistar jantan akibat paparan debu batubara yang diberikan pada wakru berbeda. Penelitian ini merupakan penelitian observasional analitik menggunakan sampel tulang tikus wistar jantan yang diambil dari Bank Jaringan PEROSI Banjarmasin. Subjek penelitian dibagi dalam tujuh kelompok yaitu, kontrol, BB1 = dosis batubara 6,25 mg/m3, BB2 = dosis batubara 12,5 mg/m3, dan BB3 = dosis batubara 25 mg/m3, yang masing-masing diberi paparan akut (14 hari) dan paparan subkronik (28 hari). Berdasarkan hasil uji Kruskal-Wallis, disimpulkan bahwa tidak terdapat perbedaan bermakna apabila didapatkan nilai p<0,05. Hasil penelitian menunjukkan perbedaan kadar kalsium tulang femur tikus wistar jantan yang tidak bermakna setelah paparan akut (p=0,739) dan subkronik (p= 0,123). Tidak terdapat perbedaan bermakna dari kadar kalsium femur tikus setelah paparan debu batubara.
Kata-kata kunci: Kadar kalsium, debu batubara, tikus wistar, akut, subkronik.
Full Text:
PDFReferences
Greenpeace. Batubara mematikan; bagaimana rakyat Indonesia membayar mahal untuk bahan bakar terkotor di dunia. Greenpeace Asia Tenggara Walhi 2010.
Setiawan B, K Nia, Y Agus, et al. Efek inhalasi debu batubara terhadap stress klorinatif dan kerusakan endotel. Journal of Indonesian Medical Association. 2011;61: 253-7.
Onder M and Seyhan O. Evaluation of occupational exposures to respirable dust in underground coal mines. Industrial Health 2009; 47: 43-49.
Hendryx M and M Ahern M. Relations between health indicators and residential proximity to coal mining in West Virginia.American Journal of Public Health 2008;98:669-671.
Finkelman RB, Orem W, Castranova V, et al. Health impacts of coal and coal use: possible solutions. International Journal of Coal Geology 2002; 50: 425-433.
Akbar IZ, Nia K, Bambang S, et al. Decreased osteoblast and increased osteoclasts in rats after coal dust exposure. Universa Medicina 2011; 30: 73-79.
Barrere F, Clemens AB, and Klass G. Bone regeneration: molecular and cellular interactions with calcium phosphates ceramics. International Journal of Nanomedics 2006; 1(3): 317-332.
Junqueira LC, Jose C, and Robert OK. Histologi dasar. Terjemahan: Jan Tambayong. Jakarta: EGC, 1998.
Noor Z, Sutiman BS, Mohammad H, et al. Assessment of microarchitecture and crystal structure of hydroxyapatite in osteoporosis. Universa Medicina 2011; 30: 29-35.
Huang X and Finkelman RB. Understanding the chemical properties of macerals and minerals in coal and its potential application for occupational lung disease prevention. Journal of toxicology and Environmental Health Part B 2008; 11(1): 45-67.
Gutowska Izabela, Zygmunt M, and Boguslaw M. The role of bivalent metals in hydroxyapatite structures as revealed by molecular modeling with the HyperChem software. Journal of Biomedical Material Research 2005; 75A: 788-793.
Vallet-Regi M and Arcos D. Biomometics nanoceramics in clinical use: from materials to applications. London: Royal Society of Chemistry, 2008.
Noor Z, Sutiman SB, MohammadH, et al. Osteoporosis: dasar patomekanisme dan peran mineral atom. Malang: Universitas Brawijaya Press, 2011.
Ilich JZ and Jane EK. Nutrition in bone health revisited: a story beyond calcium. Journal of American College of Nutrition 2000; 19(6): 715-737.
Grynpas MD. Fluoride effects on bone crystal. Journal of Bone and Mineral Research Supplement 1990; 1: 169-175.
DOI: http://dx.doi.org/10.20527/jbk.v9i2.946
Article Metrics
Abstract view : 800 timesPDF - 0 times
Refbacks
- There are currently no refbacks.
Copyright (c) 2016 Jurnal Berkala Kedokteran
Indexed by :
This work is licensed under a Creative Commons Attribution 4.0 International License.