Efek Pajanan Kadmium (Cd) terhadap Kadar Malondialdehyde (MDA) pada Ovarium Tikus Putih (Rattus norvegicus)

Muhammad Hendy Arizal, Eko Suhartono, Mohammad Bakhriansyah

Abstract


ABSTRACT: Cadmium (Cd) is heavy metal that pollutant in the environment. Chronic intake of Cd induces toxicity on liver, kidney, and ovary. Cd could damage the tissue with stress oxidative damage mechanism. Malondyaldehyde (MDA) is the product of lipid peroxidation used as a measure of lipid peroxidation and stress oxidative damage. This was an experimental laboratoric using two groups. The control group P(0) was given aquadest 2 ml per day for 4 weeks and the exposure group P(1) was given a solution of Cd with a concentration of 1.2 x 10-2 mg for 4 weeks. The results showed the mean of MDA level in the P(0) and the P(1) were 214.80 μM and 232.00 μM, respectively. Statistical analysis using Unpaired Test-T obtained the result p = 0.016 (p<0.05). It can be concluded that Cd causes increased MDA levels in rats ovary.

 

Keywords: Cadmium, malondialdehyde, ovarium, oxidative stress, rats.

 

ABSTRAK : Kadmium (Cd) merupakan logam berat bersifat polutan yang mencemari lingkungan. Paparan kronik Cd berefek toksik terhadap hati, ginjal, dan ovarium. Cd merusak jaringan melalui mekanisme stres oksidatif. Malondialdehyde (MDA) merupakan produk akhir dari peroksidasi lipid  yang menjadi parameter dalam mengukur kerusakan oksidatif. Penelitian ini bertujuan untuk mengetahui efek pajanan Cd terhadap kadar MDA ovarium tikus putih. Penelitian ini bersifat eksperimental laboratorik yang dilakukan pada 2 kelompok, yakni kelompok kontrol P(0) yang diberi aquadest sebanyak 2 ml selama 4 minggu dan kelompok perlakuan P(1) yang diberi Cd dengan konsentrasi 1,2 x 10-2 mg dalam 2 ml aquadest setiap hari selama 4 minggu. Hasil penelitian didapatkan rerata pada kelompok kontrol P(0) sebesar 214,80 ± 22,90 μM dan pada kelompok perlakuan P(1) sebesar 232,00 ± 20,40 μM dengan nilai p = 0,016 (p<0,05). Dapat disimpulkan bahwa Cd menyebabkan peningkatan kadar MDA pada ovarium tikus putih.

 

Kata-kata kunci: Kadmium, malondialdehyde, ovarium, stres oksidatif, tikus putih.

 


Full Text:

PDF

References


Yavuz C, Murat B, Onder K. environmental biology and pathophysiology of cadmium. IUFS Journal of Biology 2010; 69(1):1-5.

Boehme S,Panero M. Pollution prevention and management strategies forcadmium in the New York/New Jersey Harbor. NewYork: New York Academy of Sciences, 2003.

Godt J, Scheidig F, Siestrup CG, et al. The toxicity of cadmium and resulting hazards for human health. Journal of Occupational Medicine and Toxicology 2006;1:22.

Athena, Anwar M, Sukar. Risiko kesehatan masyarakat akibat konsumsi air bersih dan hasil laut yang mengandung kadmium di Kepulauan Seribu. Jurnal Ekologi Kesehatan 2008; 1:678-688.

Bernard A. Cadmium & its effect on human health. Indian Journal Medicine 2008; 128:557-564.

Dailiah R, Savarimuthu M, Padmalatha, et al. Cadmium toxicity exposure -induced oxidative stress in postnatal development of wistar rats. Journal of Toxicology and Environmental Health sciences 2010; 3(7):176-179.

Makoto M, Shahidur R, Mariko M, et al. Acute effect of cadmium on female reproduction in birds. Athens : WSEAS Press, 2009.

Jomova K, Valko M. Advances in metal-induced oxidative stress and human disease. Elsevier Ireland Ltd 2011; 283:65-87.

Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological fungtions and human disease. The International Journal of Biochemistry & Cell Biology 2007; 39:44-84.

Saad A, Hegazy NM, Amer HN, et al. The role of cadmium exposure on spontaneous abortion. World Journal of Medical Sciences 2012; 7(4):270-275.

Viera A, Lukacinova A, Holovska K, et al. Effect of lifetime low dose exposure to cadmium on lipid metabolism of wistar rats. Journal of Microbiology, Biotechnology and Food Sciences 2012; 2(1):293-303.

Suhartono E, Setiawan B, Yunanto A. Peran radikal bebas pada intoksikasi & patobiologi penyakit. Banjarmasin: Pustaka Banua Studio, 2009.

Suhartono E, Setiawan B. Kapita selekta biokimia radikal bebas, antioksidan dan penyakit. Banjarmasin: Pustaka Banua, 2006.

Alexander J, Benford D, Cockburn A, et al. Scientific opinion of the panel on contaminants in the food chain on a request from the europeancommission on cadmium in food. The EFSA Journal 2009; 980:1-139.

Suhartono E, Triawanti, Yunanto A, et al. Chronic cadmium hepatooxidative in rats: treatment with haruan fish extract. APCBEE Procedia 2013; 5:441-445.

Wibowo A, Rahaju FA, Firdaus RT, et al. The role of urinary cadmium and lead level on pregnant women renal function. Journal of Medical and Bioengineering 2013, 3:1.




DOI: http://dx.doi.org/10.20527/jbk.v10i12.953

Article Metrics

Abstract view : 68 times
PDF - 0 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Jurnal Berkala Kedokteran

Indexed by :

          

 

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

joomla
visitors View My Stats