Pengaruh Lama Paparan Pestisida Terhadap Aktivitas Kolinesterase, Serum Glutamic Oxaloacetic Transaminase (SGOT) dan Serum Glutamic Pyruvic Transaminase (SGPT) pada Pekerja yang Terpapar Pestisida Golongan Organofosfat

Devyana Dyah Wulandari, Andreas Putro Ragil Santoso, Devyani Diah Wulansari

Abstract


Pestisida merupakan racun yang sengaja dibuat oleh manusia untuk membunuh organisme pengganggu tanaman dan insekta penyebar penyakit. Organofosfat merupakan jenis pestisida yang paling banyak di gunakan di bidang pertanian. Paparan pestisida yang terlalu sering dapat menimbulkan berbagai masalah kesehatan. Biomarker yang paling sering dipakai dalam menentukan gejala keracunan pestisida adalah kadar kolinesterase. Oleh karena itu pada penelitian ini akan diteliti pengaruh lama paparan pestisida terhadap kolinesterase (ChE) dan transaminase (SGOT dan SGPT). Pemeriksaan kolinesterase dilakukan dengan menggunakan metode spektrofotometri menggunakan reagen thiol 5,5'-dithio-bis (2-nitro asam benzoat) (DTNB), sedangkan pemeriksaan SGOT dan SGPT dilakukan menggunakan photometer. Hasil menunjukkan bahwa tidak ada pengaruh antara lama paparan pestisida organofosfat dengan kadar SGOT, SGPT, dan kolinesterase. Hal ini disebabkan karena seluruh responden tertib dalam pemakaian APD selama bekerja.

References


Aroonvilairat, Soraya., Wannapa Kespichayawattana, Thiwaree Sornprachum,

Papada Chaisuriya, Taweeratana Siwadune and Kavi Ratanabanangkoon. 2015. Effect of Pesticide Exposure on Immunological, Hematological and Biochemical Parameters in Thai Orchid Farmers-A Cross-Sectional Study. Int. J. Environ. Res. Public Health , 12, 5846-5861

D.M., Awad, S.A., El-Fiki, Shanab, A., Hassanin, El Rahman, A. 2014. Influence Of Exposure To Pesticides On Liver Enzymes And Cholinesterase Levels In Male Agriculture Workers. Global NEST Journal, Vol 16.

EFLM (European Federation of Clinical Chemistry and Laboratory Medicine) Recommendation for Venous Blood Sampling, 2017

Jamal, F., Haque, Q.S., Singh, S., Arshad, Md. 2016. The Influence of Pesticides on Hepatic and Renal Functions in Occupational Sprayers of Rural Malihabad, Lucknow (India). Toxicol Open Access 1: 106

Jamshhidzade, Akram, Hossein Nicknahad, Mohammadi-Bardbori A, Talati M. 2009. Comparative Measurement of Serum Acetyl Cholinesterase Enzyme using Three Different Methode. Iranian Journal of Toxicology, Vol. 2, No.4

Purba, I.G. 2009. Analisis Faktor-Faktor Yang Berhubungan Dengan Kadar Kolinesterase Pada Perempuan Usia Subur Di Daerah Pertanian. [Tesis]. Program Pascasarjana Universitas Diponegoro Semarang.

Sarwar, M. 2015. The Killer Chemicals for Control of Agriculture Insect Pests: The Botanical Insecticides. International Journal of Chemical and Biomolecular Science 1(3):123-128

Skrzypczak, L.K., Sawicki, K., Czajka, M., Turski, W.A., Kruszewski, M. 2015. Cholinesterase Activity In Blood And Pesticide Presence In Sweat As Biomarkers Of Children`S Environmental Exposure To Crop Protection Chemicals. Annals of Agricultural and Environmental Medicine, Vol 22, No 3, 478–482

Soemirat, J. 2003. Toksikologi Lingkungan. Gadjah Mada University Press. Yogyakarta

Standard Operating Procedure for Collecting, Proccessing, and Storage of Blood Samples, 2017




DOI: http://dx.doi.org/10.20527/jstk.v14i1.6516

Article Metrics

Abstract view : 1056 times
PDF (Bahasa Indonesia) - 687 times

Refbacks

  • There are currently no refbacks.


Dear colleagues,

We are pleased to inform you that the result of our application for journal accreditation has been finalized through the Arjuna system. Based on the assessment result, Jurnal Ilmiah Berkala Sains dan Terapan Kimia  has now been officially accredited by Ministry of Research, Technology, and Higher Education of Republic of Indonesia with a predicate of SINTA 3. The official letter of the accreditation result is available through this link.

We hope that this accreditation result will lead us to more publications with better quality.

Sincerely,

Editorial Team of JIB Sains dan Terapan Kimia.


Alamat Redaksi:
Jl. A. Yani, KM. 36, 
PROGRAM STUDI KIMIA
Kampus Fakultas Matematika dan Pengetahuan Alam, Gedung I,
Universitas Lambung Mangkurat, Banjarbaru (73714)

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.