Inhibisi Enzim α-Glukosidase oleh Bacillus Megaterium ITU 9 Isolat Sumber Air Panas Sumatera Utara

Finna Piska; Chrismis Novalinda Ginting; Harmileni Harmileni; Edy Fachrial

doi:10.20527/jps.v11i1.17045

Inhibisi Enzim α-Glukosidase oleh Bacillus Megaterium ITU 9 Isolat Sumber Air Panas Sumatera Utara

Finna Piska, Chrismis Novalinda Ginting, Harmileni Harmileni, Edy Fachrial

Abstract

Diabetes adalah penyakit multifaktor yang insidensinya meningkat dengan pesat di negara berkembang. Diabetes mellitus (DM) tipe 2 adalah jenis diabetes yang paling umum ditemui, dimana penyakit ini erat kaitannya dengan kemajuan industri dan teknologi sehingga mempengaruhi gaya hidup seseorang. Penanggulangan penyakit ini dapat dilakukan dengan menghambat aktivitas α-glukosidase. Beberapa mikroorganisme terutama dari sumber air panas dapat dimanfaatkan sebagai penghasil senyawa inhibitor α-glukosidase. Pada penelitian sebelumnya isolat Bacillus megaterium ITU 9 merupakan isolat yang diisolasi dari sumber air panas. Pada penelitian ini diuji kemampuan isolat Bacillus megaterium ITU 9 sebagai penghasil inhibitor α-glukosidase dengan metode sumur. Daya persen inhibisi supernatan B. megaterium ITU 9 terhadap kerja enzim α-glukosidase adalah sebesar 46,67%, sedangkan daya persen inhibisi acarbose yaitu 70%. Dalam hal ini B. megaterium ITU 9 termofilik memiliki daya inhibisi terhadap enzim α-glukosidase.

Kata Kunci: Bakteri Termofilik, Inhibitor, Akarbose, Metode Sumur, Persen Inhibisi

Diabetes is a multifactorial disease whose incidence is increasing rapidly in developing countries. Diabetes mellitus (DM) type 2 is the most common type of diabetes, where this disease is closely related to industrial and technological advances, thus affecting a person's lifestyle. This disease can be controlled by inhibiting α-glucosidase activity. Several microorganisms, especially from hot springs, can be used to produce α-glucosidase inhibitor compounds. In previous research, the Bacillus megaterium ITU 9 isolate was an isolate isolated from hot springs. In this study, the ability of the Bacillus megaterium ITU 9 isolate to produce α-glucosidase inhibitors was tested using the well method. The percent inhibition power of B. megaterium ITU 9 supernatant on the action of the α-glucosidase enzyme was 46.67%, while the percent inhibition power of acarbose was 70%. In this case, the thermophilic B. megaterium ITU 9 has inhibitory power against the α-glucosidase enzyme.

Keywords

Inhibitor, Thermophilic Bacteria, Acarbose, The Well Method, The Percent Inhibition

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DOI: http://dx.doi.org/10.20527/jps.v11i1.17045