Rambusa (Passiflora foetida L) vs. Free Radicals:  In Vitro Study with DPPH Method

Dewi Sari Mulia; Evi Mulyani; Muhammad Ikhwan Rizki; Mohammad Rizki Fadhil Pratama

doi:10.20527/jps.v6i2.7344

Rambusa (Passiflora foetida L) vs. Free Radicals: In Vitro Study with DPPH Method

Dewi Sari Mulia, Evi Mulyani, Muhammad Ikhwan Rizki, Mohammad Rizki Fadhil Pratama

Abstract

ABSTRAK

Rambusa (Passiflora foetida L) adalah tumbuhan obat yang banyak terdapat di berbagai daerah di Indonesia, termasuk di Kalimantan Tengah. Beberapa bagian tumbuhan dari rambusa diketahui memiliki aktivitas antioksidan termasuk di bagian daunnya. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dari daun rambusa yang berasal dari Kalimantan Tengah. Metode yang digunakan adalah metode 2,2-diphenyl-1-picrylhydrazyl (DPPH) yang diukur serapannya dengan Spektrofotometer UV Vis. Hasil yang diperoleh menunjukkan nilai IC₅₀ dari ekstrak etanol daun rambusa senilai 93,269 μg/mL. Meskipun nilai IC₅₀ yang diperoleh lebih rendah dibandingkan senyawa antioksidan standar seperti kuersetin, nilai IC₅₀ dari ekstrak etanol daun rambusa asal Kalimantan Tengah masih lebih tinggi dibandingkan daun rambusa yang diperoleh dari daerah lain

Kata Kunci—antioksidan, DPPH, Kalimantan Tengah, Passiflora foetida, rambusa

ABSTRACT

Rambusa (Passiflora foetida L) is a medicinal plant that is widely found in various regions in Indonesia, including in Central Kalimantan. Some parts of the plant of rambusa are known to have antioxidant activities including in the leaves. This study aims to determine the antioxidant activity of rambusa leaves from Central Kalimantan. The method used was 2,2-diphenyl-1-picrylhydrazyl (DPPH) method which was measured by UV Vis spectrophotometer. The results obtained showed IC₅₀ values of ethanol extract of rambusa leaves worth 93.269 μg/mL. Although the IC₅₀ values obtained were lower than the standard antioxidant compounds such as quercetin, the IC₅₀ value of the ethanol extract of the leaves of rambusa from Central Kalimantan was still higher than that of the rambusa leaves obtained from other regions or some other medicinal plant extracts.

Keywords— antioxidants, DPPH, Central Kalimantan, Passiflora foetida, rambusa

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DOI: http://dx.doi.org/10.20527/jps.v6i2.7344