PENGARUH GEL KOMBINASI KITOSAN SISIK IKAN HARUAN (CHANNA STRIATA) DAN HIDROKSIAPATIT TERHADAP CRYSTALLINITY INDEX ENAMEL

Cetri Bonifasia Sihombing, Deby Kania Tri Putri, Yusrinie Wasiaturrahmah, Amy Nindia Carabelly, Alfi Alfi Yasmina

Abstract


Background: Dental caries is a major oral health problem with a prevalence of 43.44% in Indonesia. Commonly used remineralizing agents such as CPP-ACP may trigger allergic reactions in patients with Cow's Milk Allergy (CMA). A combined gel of haruan fish scale chitosan (Channa striata) and hydroxyapatite was developed as a safe and effective alternative remineralization agent. Objective: To analyze the effect of combined gel of haruan fish scale chitosan (Channa striata) and hydroxyapatite on enamel Crystallinity Index (CI) as an indicator of remineralization potential. Methods: A true experimental laboratory study was conducted using 12 maxillary first premolar samples divided into four groups: negative control, combined gel of 5% chitosan and 10% hydroxyapatite, combined gel of 10% chitosan and 10% hydroxyapatite, and positive control (CPP-ACP). Demineralization was performed with 37% phosphoric acid for 15 seconds. CI was measured using ATR-FTIR. Data were analyzed using One Way ANOVA followed by Bonferroni post hoc test. Results: The highest CI was observed in the 10% chitosan and 10% hydroxyapatite group (mean 4.18±0.76), followed by 5% chitosan and 10% hydroxyapatite (2.94±0.16), CPP-ACP (2.57±0.37), and the lowest in the negative control (1.62±0.43). One Way ANOVA revealed statistically significant differences between groups (p=0.003). Conclusion: The combined gel of haruan fish scale chitosan (Channa striata) and hydroxyapatite significantly increased enamel Crystallinity Index compared to the untreated group. The combination of 10% chitosan and 10% hydroxyapatite demonstrated the highest CI improvement, surpassing CPP-ACP, indicating its potential as an alternative topical remineralizing agent in preventive dentistry.

Keywords : Channa striata fish scale chitosan, Crystallinity Index, demineralization, enamel remineralization, hydroxyapatite.


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DOI: https://doi.org/10.20527/dentin.v10i2.20717

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