AKTIVITAS ANTIBIOFILM EKSTRAK AIR DAUN Syzygium polyanthum (Wight) Walp TERHADAP PEMBENTUKAN BIOFILM Candida albicans ATCC.10231 IN VITRO

Abstract

Abstract: Candida albicans has ability form biofilms which causes resistance antifungal. The leaves of Syzygium polyanthum (Wight) Walp (S. polyanthum) contain antifungal compounds such as flavonoids, alkaloids, saponins, tannins which developed as anti-biofilm. This study aims to analyse the antibiofilm activity of the extract of leaf water S. polyanthum on C. albicans biofilm formation based on the Minimum Biofilm Inhibitory Concentration (MBIC) and Mean Gray Value (MGV) parameters. Experimental research with a posttest only control group design using the tube test method. The treatment was conducted in three repetitions, namely the extract of leaf water S. polyanthum at 6.25%, 12.5%, 25%, 50%, 75%, 100%, with control groups of fluconazole 5%, sterile distilled water, and TSB+Glucose5%. The tube test results showed that the MBIC of the extract of leaf water S. polyanthum on C. albicans biofilm was 12.5%. The analysis results of the One-way Anova and Post-hoc Duncan tests on the MGV data showed significant antibiofilm activity (p<0,05). The MGV value of the extract of leaf water S. polyanthum 75% (142.128) was equivalent to the MGV of fluconazole 5% (142.052), and the highest MGV was produced by the extract of leaf water S. polyanthum 100%. In conclusion, the extract of leaf water S. polyanthum has antibiofilm activity in inhibiting the formation of C. albicans biofilm.

Keywords:  Biofilm, C. albicans, MBIC, MGV, S. Polyanthum

Abstrak: Candida albicans memiliki kemampuan membentuk biofilm yang menimbulkan resistensi terhadap antifungi. Daun Syzygium polyanthum (Wight) Walp (S. polyanthum) mengandung senyawa antifungi flavonoid, alkaloid, saponin, dan tanin yang berefek sebagai antibiofilm. Penelitian ini bertujuan menganalisis aktivitas antibiofilm dari ekstrak air daun S. polyanthum terhadap pembentukan biofilm C. albicans, berdasarkan parameter Minimum Biofilm Inhibitory Concentration (MBIC) dan Mean Gray Value (MGV). Penelitian eksperimental dengan rancangan posttest only control group design menggunakan metode  tube test. Perlakuan dilakukan dalam tiga kali pengulangan, yaitu ekstrak air daun S. polyanthum 6,25%, 12,5%, 25%, 50%, 75%, 100% dengan kontrol flukonazol 5%, aquadest steril, dan TSB+Glucose 5%. Hasil tube-test didapatkan MBIC dari ekstrak air daun S. polyanthum pada biofilm C. albicans adalah 12,5%.  Hasil analisis uji One-way Anova dan Post-hoc Duncan pada data MGV didapatkan aktivitas antibiofilm yang bermakna (p.005). Nilai MGV dari ekstrak air daun S. polyanthum 75% (142.128) setara MGV Flukonazol 5% (142.052), dan MGV paling tinggi dihasilkan ekstrak S. polyanthum 100%. Kesimpulan, ekstrak air daun S. polyanthum memiliki aktivitas antibiofilm dalam menghambat pembentukan biofilm C. albicans.

Kata-Kata Kunci:  Biofilm, C. albicans, MBIC, MGV, S. Polyanthum