Khairunnisa Puspita Sari, Didit Aspriyanto, Beta Widya Oktiani


Background: Periodontitis is a periodontal disease that can cause an increase in Reactive Oxygen Species (ROS). The increase in ROS concentration will cause oxidative stress which has a role in DNA damage. In the case of periodontitis, panoramic radiography plays an important role in showing generalized horizontal bone loss. Radiation on panoramic radiography may cause DNA damage. DNA damage that occurs due to periodontitis or panoramic radiographic radiation exposure is characterized by the formation of micronuclei in gingival epithelial cells. Objective: To determine the effect of panoramic radiography on the number of micronuclei in Wistar rats with periodontitis. Method: This study was true experimental with post-test only and control group design. This study used 15 male Wistar rats which divided into 5 groups. Result: The average number of micronuclei in the normal group and 1 time exposures was 4 compared to the periodontitis group without exposure was 1.67, periodontitis and 1 time exposures was 8, periodontitis and 2 times the exposure was 15.67, and in the periodontitis and 3 times the exposure was 42.67. Result of One-Way Annova test and Post Hoc Bonferroni test indicated that signigicant changes in the number of micronuclei was seen between the normal group with 1 time exposure to the periodontitis group with 2 times and 3 times exposure and int the periodontitis group without exposure to the periodontitis group with 2 times and 3 times exposure. Conclusion: Panoramic radiograph X ray radiation and periodontitis can cause changes in the number of micronuclei in wistar rats.


Keywords: , Micronucleus, Panoramic Radiography, Periodontitis.

Full Text:



Perez Z, Garcia O, Ramos L, Velazquez G, Meda G, Aguilar R, Gonzalez Z. Increased Micronuclei and Nuclear Abnormalities in Buccal Mucosa and Oxidative Damage in Saliva From Patient With Chronic and Aggressive Periodontal Diseases. Journal of Periodontal Research. 2014: 1-5.

Ekaputri S, Masulili SL. Cairan Sulkus Gingiva Sebagai Indikator Keadaan Jaringan Periodontal. Maj Ked Gi. 2010; 17(1); 75.

Tadin A, Gavic L, Roguljic M, Jerkovic D, Zeljezic D. Nuclear Morphological Changes in Gingival Epithelial Cells of Patients With Periodontitis. Clinical Oral Investigations Original Article. 2019: 1-2.

Saberi BV, Nermati S, Javanmard A. Assessment of Digital Panoramic Radiography’s Diagnostic Value in Angular Bony Lesions With 5 mm Or Deeper Pocket Depth In Mandibular Molars. Dental Research Journal. 2017; 14(1): 33.

Salman A, Meethil A. An Unusual Presentation of Generelized Aggressive Periodontitis With Multiple Impacted Supernumerary Teeth. European Journal of Dentistry. 2012; 6.

Sheikh S, Pallagatti S, Grewel H, Kalucha A, Kaur H. Genotoxicity of Digital Panoramic Radiography on Oral Epithelial Tissues. Quintessense International. 2012; 43(8): 719.

Noorma, Sukmana BI, Saputera D. The Influence Between The Length Of Radiographer Working Time And The Reduction Of Salivary pH. Dentino Jurnal Kedokteran Gigi. 2018; 3(1). 127-128.

Susanti NT, Prasetyarini S, Shita ADP. Pengaruh Pajanan Radiasi Sinar-X dari Radiografi Panoramik terhadap pH Saliva. e-Jurnal Pustaka Kesehatan. 2016; 4(2): 353.

Whaites E, Drage N. Essentials of Dental Radiography and Radiology 5th Edition. Churchill Livingstone Elsevier. China: 2013.p. 65;66.

Shantiningsih RR, Suwaldi, Astuti I, Mudjosemedi M. Peningkatan Jumlah Mikronukleus Pada Mukosa Gingiva Kelinci Setelah Paparan Radiografi Panoramik. Maj Ked Gi. 2013; 20(2): 119-125.

Saputra D, Astuti ER, Daraninggar A, Alam AR, Satria FA, Aulia M. The Effect Of Mangosteen Pericarp (Garcinia mangostana L.) Extract Mucoadhesive Gingival Patch On The Mda Levels And The Number Of Micronuclei Due To Panoramic Radiography Radiation. Indonesian Journal of Dental Medicine (IJDM). 2019; 2(4): 508-514.

Setyawati HA, Dewi N, Oktaviyanti IK. Analisis Sitogenik Mikronukleus Bukal Pada Orang Menginang dan Tidak Menginang. Dentino Jurnal Kedokteran Gigi. 2016; 1(1): 43.

Ramos MA, Cury FDP, Neto CS, Marques MMC, Silveira HCS. Micronucelus Evaluation of Exfoliated Buccal Epithelial Cells Using Liquid-Based Cytology Preparation. Acta Cytologica. 2014: 582-584.

Rahmah N, Dewi N, Rahardja SD. Analisis Sitogenik Mikronukleus Mukosa Bukal Pada Perokok Aktif dan Pasif. Dentino Jurnal Kedokteran Gigi. 2016; 1(1): 16-19.

Badan Pengawas Tenaga Nuklir. Pedoman Teknis Penyusunan Tingkat Panduan Diagnostik atau Diagnostic Reference Level (DRL) Nasional. 2019: p.2.

Susantiningsih T. Obesitas dan Stres Oksidatif. JuKe Unila. 2015; 5(9): 90.

Wang Y, Andrukhov O, Fan XR. Oxidative Stress and Antioxidant System in Periodontitis. Frontiers in Physiology. 2017; 8.

Perez Z, Gonzalez G, Ramos L, Velazquez G, Parada S. Periodontal Disease and Nuclear and Oxidative DNA Damage. Insights into Various Aspects of Oral Health. 2017: 29-30.

White SC, Pharoah MJ. Oral Radiologi Principles and Interpretation 7th Edition. Elsevier. China: 2013.p.33.

Fenech M, Kirsch-Volders M, Natarajan A. T., Surralles J., Crott J. W, Parry J. Molecular Mechanism of Micronucleus, Nucleoplasmic Bridge and Nuclear Bud Formation In Mammalian And Human Cells. Mutagenesis. 2011; 26(1): 126.

Berniyanti T. Biomarker Toksisitas Paparan Logam Tingkat Molekuler. Pusat Penerbitan dan Percetakan Universitas Airlangga (AUP): Surabaya; 2018.p.60.

Bastos-Aires D, Alvaro A, Mara LP, Daniel PM, Alexandra T. Preliminary Study of Micronuclei Levels in Oral Exfoliated Cells From Patients With Periodontitis. J Dent Sci. 2013; 8: 200-204.

Cerqueira EMM, Meireles JRC. The Use of the Micronucleus Test to Monitor Individuals at Risk of Oral Cancer. The Research and Biology of Cancer. 2014: 2-3.



Article Metrics

Abstract view : 35 times
PDF - 11 times


  • There are currently no refbacks.


Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia
Telp/Fax. (0511) 3255444;

E-mail. /


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.