THE EFFECT OF BINJAI (Mangifera caesia) LEAVES EXTRACT GEL TO FIBROBLAST CELL NUMBER ON INCISION WOUND

Noor Hildayanti, Irham Taufiqurrahman, Renie Kumala Dewi

Abstract


Background: The ethanol extract of binjai leaves contains bioactive compounds that are saponin, tannin, triterpenoid, steroid, alkaloid and flavonoid which potentially as an anti-inflammation, anti-bacterial, and antioxidant. It can fasten and control the process of scar healing. The binjai leaves extract was made into gel preparation. The binjai leaves extract gel can affect fibroblast cell number during the proliferation phase. Purpose: To analyze the effect of binjai (Mangifera caesia) leaves extract gel to the fibroblast cell number on male wistar rats (Rattus norvegicus) incision wound.  Methods: This research design was a true laboratory experimental research with post-test only with control group design which used 24 male wistar rats and divided into 4 groups: the negative control group that was given the placebo gel and the treatment group that was given the binjai leaves extract gel with 5%, 10%, and 15% concentrations. Results: The result of Two-way ANOVA statistic test shows that there is a meaningful difference in the treatment group on the 7th day (p=0,000) and on the 14th day (p=0,000). The Post-Hoc Bonferroni test for fibroblast cell number on the 7th and 14th day shows that there is a meaningful difference (p<0,05) between the negative control group and the treatment group. Conclusion: Binjai leaves extract gel have an effect to the fibroblast cell number on male wistar rats incision wound with the most effective concentration of 15%.

 

Keywords: Binjai leaves extract gel, Fibroblast cell number,  Flavonoid, Incision wound healing

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Sorongan RS, Pangemanan DHC, Siagian KS. Efektivitas Perasan Daun Pepaya Terhadap Aktivitas Fibroblas Pasca Pencabutan Gigi Pada Tikus Wistar Jantan. Pharmaco jurnal Ilmiah Farmasi – Unsrat. 2015; 4 (4): 52-57.

Sabirin IPR, Maskoen AM, Hernowo BS. Peran Ekstrak Etanol Topikal Daun Mengkudu (Morinda citrifolia L.) pada Penyembuhan Luka Ditinjau dari Imunoekspresi CD34 dan Kolagen pada Tikus Galur Wistar. MKB. 2015; 45 (4): 226-233. Sapara TU, Waworuntu O, Juliatri. Efektivitas Antibakteri Ekstrak Daun Pacar Air (Impatiens balsamina L.) Terhadap Pertumbuhan Phorphyromonas gingivalis. Jurnal Ilmiah Farmasi. Desember 2016; 4(5). 2302-2493.

Dewi PS. Efektifitas Ekstrak Lidah Buaya Terhadap Jumlah Sel Fibroblas Pada Proses Penyembuhan Luka Insisi Marmut. Intisari Sains Medis 2018; 9 (3): 51-54.

Khowariro MI. Pengaruh Pemberian Minyak Buah Merah Terhadap Jumlah Sel Fibroblas Pada Punggung Tikus Jantan Galur Wistar Dengan Luka Bakar Derajat IIA. Jurnal Pendidikan dan Humaniora. 2018; 2 (3): 259-272.

Masir O, Menkher Manjas M, Putra AE, Agus S. Pengaruh Cairan Cultur Filtrate Fibroblas (CFF) Terhadap Penyembuhan Luka; Penelitian eksperimental pada Rattus Norvegicus Galur Wistar. Jurnal Kesehatan Andalas. 2012; 1 (3): 112-117.

Ardiana T, Kusuma ARP, Firdausy MD. Efektivitas Pemberian Gel Binahong (Anredera Cordifolia) 5% Terhadap Jumlah Sel Fibroblas Pada Soket Pasca Pencabutan Gigi Marmut (Cavia Cobaya). Odonto Dental Journal. 2015; 2 (1): 64-70.

Shiel WC. Definition of Adjuvant Therapy. Available from https://www.rxlist.com/script/main/art.asp?articlekey=2151. Reviewed November, 2018.

Darmayanta HI, Edyson, Budiarti LY. Aktivitas Hambat Ekstrak Etanol Daun Binjai (Mangifera Caesia Jack) Terhadap Salmonella typhi Dan Shigella dysentriae In Vitro. Homeostasis. 2019; 2 (2): 233-240.

Mohammed M.S, Osman W.J.A, Garelnabi E.A.E, Osman Z, Osman B, Khalid H.S, Mohammed M.A. Secondary Metabolites As Anti-Inflammatory Agents. The Journal of Phytopharmacology. 2014; 3 (4): 275-285.

Yunanda V, Rinanda T. Aktivitas Penyembuhan Sediaan Topikal Ekstrak Bawang Merah (Allium Cepa) Terdapat Luka Sayat Kulit Mencit (Mus Musculus). Jurnal Veteriner. 2016; 7(14): 606-614.

Anwar K, Fadlillaturrahmah, Sari DP. Analisis Kandungan Flavonoid Total Ekstrak Etanol Daun Binjai (Mangifera Caesia Jack.) Dan Pengaruhnya Terhadap Kadar Glukosa Darah Tikus Yang Diinduksi Fruktosa-Lemak Tinggi. Jurnal Ilmiah Ibnu Sina. 2017; 2 (1): 20-30.

Syafarina M, Taufiqurrahman I, Edyson. Perbedaan Total Flavonoid Antara Tahapan Pengeringan Alami Dan Buatan Pada Ekstrak Daun Binjai (Mangifera caesia). Dentino Jurnal Kedokteran Gigi. April 2017; 1(1): 84-88.

Palumpun EF, Wiraguma AAGP, Pangkahila W. Pemberian ekstrak daun sirih (Piper betle) secara topikal meningkatkan ketebalan epidermis, jumlah fibroblas, dan jumlah kolagen dalam proses penyembuhan luka pada tikus jantan galur Wistar (Rattus norvegicus). Jurnal e-Biomedik. 2017; 5 (1).

Sugiaman VK. Peningkatan Penyembuhan Luka di Mukosa Oral Melalui Pemberian Aloevera (Linn.) Secara Topikal. JKM. 2011; 11(1): 70-79.

Rosita JM, Taufiqurrahman I, Edyson. Perbedaan Total Flavonoid Antara Metode Maserasi Dengan Sokletasi Pada Ekstrak Daun Binjai (Mangifera Caesia) (Studi Pendahuluan Terhadap Proses Pembuatan Sediaan Obat Penyembuhan Luka). Jurnal Kedokteran Gigi. April 2017; 1(1): 100-105.

Napanggala A, Susianti, Apriliana E. Pengaruh Pemberian Getah Tanaman Jarak Pagar (Jatropha Curcas L.) Secara topikal Terhadap Tingkat Kesembuhan Luka Iris Pada Tikus Putih Jantan Galur Sprague Dawley. Jurnal Medika. 2014; 3(5): 26-35.

Kurnia PA, Ardhiyanto HB, Suhartini. Potensi Ekstrak Teh Hijau (Camellia sinensis) terhadap Peningkatan Jumlah Sel Fibroblas Soket Pasca Pencabutan Gigi pada Tikus Wistar. e-Jurnal Pustaka Kesehatan. Januari 2015; 3 (1): 122-127.

Sumbayak EM. Fibroblas: Struktur dan Peranannya dalam Penyembuhan Luka. Jurnal Kedokteran Meditek. 2015; 21 (57).

Rosa SA, Adi S, Achadiyani A, Khairani AF, Lantika UA. Efek Gel Kentang Kuning (Solanum Tuberosum L) Terhadap Proses Penyembuhan Luka Pada Mencit (Mus Musculus). E-Journal Unisba. 2018; 6(1): 21-27




DOI: http://dx.doi.org/10.20527/dentino.v6i2.12000

DOI (PDF): http://dx.doi.org/10.20527/dentino.v6i2.12000.g7621

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