.png)
EFFECT OF CHANNA MICROPELTES IN THE GRANULATION, FIBROSIS AND NECROSIS OF DIABETIC WOUND HEALING
Abstract
Background: Diabetes mellitus (DM) is characterized by persistent hyperglycemia. The symptoms of DM is delayed wound healing. Delayed wound healing in diabetes will increase the risk of wound complications that will hinder the healing process, and lead to complications such as fibrosis and necrosis. The use of Channa Micropeltes (CM) extract at a dose of 16 mL/kg BW orally is proven to general optimal wound closure and wound contraction clinically in Wistar rats as diabetic model on day 14.However, the histopathological description of wound healing is yet to be known. Objective: To analyze the effect of 16 mL/kg BW dose of CM extract on histopathological changes of wound healing granulation tissue, fibrosis and necrosis in diabetic Wistar rats on day 14. Materials and Methods: This study was a true experimental design with a post-test only control group design. The samples were divided into two groups, namely the diabetic model group given CM extract at 16 mL/kg BW dose for 14 days, and the diabetic model group given BR2 feed only for 14 days. Results: The results of Mann-Whitney U test showed p = 0.003 for granulation tissue, p = 0.411 for fibrosis and p = 1 for necrosis, with the level of significance was p<0.05. Conclusion: The CM extract at 16 mL/kg BW dose affects the histopathological description of granulation tissue which presents a better result than those in the control group but did not affect the features of fibrosis and necrosis in diabetic Wistar rats on day 14.
Keywords: Channa Micropeltes, Diabetic wound, Fibrosis, Granulation tissue, Necrosis.
Full Text:
PDFReferences
International Diabetes Federation. IDF Diabetes Atlas Ninth Edition. Dunia: IDF; 2019. p.12, 39.
Rosyid FN, Etiology, pathophysiology, diagnosis and management foot ulser. International Journal of Research in Medical Sciences 2017;5(10):4206-4213.
Biswas S and Chakrabarti S. Increased Extracellular Matrix Protein Production in Chronic Diabetic Complications: Implications of Non-Coding RNAs. Non-coding RNA 2019; 5(30):1-25.
Lévigne D, Tobalem M, Modarressi A, Pittet-Cuénod B. Hyperglycemia Increases Susceptibility to Ischemic Necrosis. BioMed Research International 2013;2013:1-5
Sukmono T dan Margaretha M. Ikan Air Tawar di Ekosistem Bukit Tigapuluh. Jambi: Yayasan Konservasi Ekosistem Sumatera dan Franfrut Zoological Society; 2017. p.85
Firlianty, Supriyatno E, Nursyam H, Hardoko, Mustafa A. Chemical Composition and Amino Acid Profile of Channidae Collected From Central Kalimantan, Indonesia. International Journal of Science and Technology IJSTE 2013;2(4):25-29.
Apriasari ML, Ainah Y, Febrianty E, Carabelly AN. Antioxidant Effect of Channa Micropeltes in Diabetic Wound of Oral Mucosa. International Journal of Pharmacology 2019;15(1):143-137.
Royyana A, Carabelly AN, Aspriyanto D. The Influence Of Toman Fish (Channa Micropeltes) Extract On The Number Of Neovascular In Diabetes Mellitus Wound Healing. Jurnal Kedokteran Gigi Dentino 2018;2(3):101-107.
Apriasari ML, Syahadati MA, Carabelly AN. Clinical Analysis of Channa Micropeltes for Treating Wound of Diabetes Mellitus. Jurnal Berkala Kedokteran 2020;16(1):1-10.
Albaayit SFA, Abba Y, Rasedee A, Abdullah N. Drug Des Devel Ther 2015;9:3507-3518.
Ding S, Walton KLW, Blue RE, MacNaughton K, Magness ST, Lund PK. Mucoosal Healing and Fibrosis after Acute or Chronic Inflammation in Wild Type FVB-N Mice and C57BL6 Procollagen α1(I)-Promoter-GFP Repoter Mice. PloS ONE 2012;7(8):e42568.
Bilge NSY, Dundar E, Mutlu FS, Gulbas Z. Erythropoietin Improves the Healing of Skin Necrosis Resulting From Doxorubicin Extravasation in a Rat Model. Current Therapeuic Research 2011;72(4):141-149.
Suryadi IA, Asmarajaya AAGN, Maliawan S. Wound Healing Process and Wound Care. E-Jurnal Medika Udayana 2013;2(2):1-19.
Gonzalez ACDO, Andrade ZDA, Costa Tf, Medrado ARAP. Wound Healing- A Literature Review. An Bras Dermatol 2016;91(5):614-620.
Shita ADP. Perubahan Level TNF-α dan IL-1 pada Kondisi Diabetes Mellitus. Jurnal Kedokteran Gigi UNEJ 2015;1(1):1-7.
Nowotny K, Jung T, Hohn A, Weber D, Grune T. Advanced Glycation End Products and Oxidative Stress in Type 2 Diabetes Mellitus. Biomolecules 2015; 5: 194-222.
Volpe CMO, Delfino PHV, Anjos PMFD, Machado JAN. Cellular Death, Reactive Oxygen Species (ROS) and Diabetic Complications. Journal of the Cell Death Differentiation Association 2018;9(119):1-9.
Irwanda WF, Andrie M, Luliana S. Uji Efek Penyembuhan Luka Fase Air Ekstrak Ikan Toman pada Tikus Putih Jantan Wistar yang diberi Luka Sayat. Jurnal Mahasiswa Farmasi Fakultan Kedokteran Untan 2015;3(1):1-13
Fajriani N, Carabelly AN, Apriasari M. The Effect of Toman Fish Extract (Channa Micropeltes) Neutrophil in Diabetes Mellitus Wound Healing. Jurnal Kedokteran Gigi DENTINO 2018;3(1):15-21.
Primadina N, Basori A, Perdanakusuma DS. Proses Penyembuhan Luka Ditinjau dari Aspek Mekanisme Seluler dan Molekuler. Qanun Medika 2019;3(1):31-43.
Nicodemus, Andrie M, Luliana S. Uji Efek Penyembuhan Luka Sayat Ekstrak Ikan Toman secara Oral pada Tikus Putih Jantan Wistar. Jurnal Mahasiswa Farmasi Fakultan Kedokteran Untan 2014;1(1):1-14.
Tungadi R, Attamimi F, Sabu EF, Nugraha E. Percepatan Penyembuhan Luka oleh Krim Ikan Gabus (Ophiocephalus striatus) terhadap Luka Kulit Kelinci secara Histopatologi. Jurnal Ilmu Kefarmasian Indonesia 2011;9(2):91-97.
Hanna VS, Hafez EAZ. Synopsis of Arachidonic Acid Metabolism: A Review. Journal of Advanced Research 2018; 11:23-32.
Jones RE, Foster DS, Longaker MT. Management of chronic wounds- 2018. The journal of the American Medical Association 2018;320(14):1481-1482.
Nascimento OJM, Pupe CCB, Cavalcanti EBU. Diabetic neuropathy. Rev Dor. São Paulo, 2016;17(Suppl 1): S46-51.
DOI: http://dx.doi.org/10.20527/dentino.v6i2.12001
DOI (PDF): http://dx.doi.org/10.20527/dentino.v6i2.12001.g7622
Article Metrics
Abstract view : 294 timesPDF - 158 times
Refbacks
- There are currently no refbacks.
Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia
E-mail. [email protected]
Website. fkg.ulm.ac.id
This work is licensed under a Creative Commons Attribution 4.0 International License.