THE TOXICITY OF METHANOL EXTRACT OF MAULI BANANA STEM (Musa acuminata) AGAINST BONE MARROW MESENCHYMAL STEM CELL IN VITRO

Amy Nindia Carabelly, Suhartono Taat Putra, Ketut Suardita

Abstract


ABSTRACT
The latest regeneration therapy has developed towards the usage of Mesenchymal stem cells (MSC) including for aggressive periodontitis, but limited amounts of MSC need extra growth factor in cells culture process. Growth factor is quite expensive so an alternative source is needed. Mauli banana stem is a proven antioxidant and has the most bioactive tannin. Methanol extract of mauli banana stem is not toxic towards fibroblast cell BHK 21 with 25% concentrate, but there is no research about the toxicity of methanol extract of mauli banana stem against MSC. Purpose: To analyze the toxicity of methanol extract of mauli banana stem against MSC in vitro. Method and source: True experimental using the posttest only control group design. MSC culture with treatment methanol extract of mauli banana stem with dosage 2,5 mg/ml; 5 mg/ml; 7,5 mg/ml; 10 mg/ml. Treatment Con A5 μg/ml is used for positive control group and is not treated as negative control group. Each group is incubated for 24 hours and 48 hours, then it is given reagent MTT and is read with ELISA reader. Result: Kruskal-Wallis and independent-sample T-test result shows that there is a significant difference between treatment group and control group. Conclusion: Methanol extract of mauli banana stem with dosage 2.5 mg/ml; 5 mg/ml; 7.5 mg/ml; 10 mg/ml is toxic towards MSC in vitro under treatment for 24 hours and 48 hours.
Key words : Mauli banana stem, Mesenchymal stem cells, Toxicity

Full Text:

PDF

References


Garrett S. Periodontal regeneration around natural teeth. Ann Periodontol.1996; 1: 621-70.

Francisco M, Pedro B, Pablo de Grado, Vicente F, Francisco JGL. Periodontal Regeneration in clinical practive. Periodontal regeneration E. 2006; 282-290.

Ripamonti U, Vukicevic S. Bone morphogenetic proteins: from developmental biology to molecular therapeutics. S Afr J Sci. 1995; 91:277–80.

Kawaguchi H, Hirachi A, Hasegawa N, Iwata T, Hamaguchi H, Shiba H, Takata T, Kato Y and Kurihara H. J.Periodontol. 2004; 75 : 1281-1287.

Friedenstein A, Chailakhjan R, Lalykina K. The development of fibroblastcolonies in monolayer cultures of guinea-pig bone marrowand spleen cells. Cell Proliferation. 1970; 3 (4): 393–403.

Stolzing A, Jones E, McGonagle D, Scutt A. Age-related changes in Human bone marrow-derived mesenchymal stem cells: consequences for cell therapies. Mechanisms of Ageing and Development. 2008; 129 (3): 163–173.

Maulana R, Widodo, Cholil. Efektivitas ekstrak methanol getah batang pisang terhadap penyembuhan luka pada mukosa mencit. Jurnal Kedokteran Gigi Dentino. 2013; 1(1) : 94-100.

Apriasari ML (b), Fadhilah A, Carabelly AN. Aktifitas antibakteri ekstrak metanol batang pisang mauli (Musa sp) terhadap Streptococcus mutans. Dentofacial Jurnal Kedokteran Gigi UNHAS. 2013; 12(3): 148- 151.

Septianoor MH. Uji efektifitas antifungi ekstrak batang pisang Mauli (Musa sp) terhadap Candida albicans in vitro. Jurnal PDGI. 2014; 62 (1): 7-10

Apriasari ML, Iskandar, Suhartono E. Bioactive compounds and antioxidant activity of methanol extract Mauli bananas (Musa sp) stem. International Journal of Bioscience, Biochemistry and Bioinformatics. 2014; 4(2) : 110-115.

Liu YZ, Cao YG, Ye JQ, Wang WG, Song KJ, Wang XL, Wang CH, Li RT and Deng XM. Immunomodulatory effects of proanthocyanidin A-1 derived in vitro from Rhododendron spiciferum, Fitoterapia. 2009; 81: 108–114.

Okuda T, Ito H. Tannins of Constant Structure in Medicinal and Food Plants-Hydrolyzable Tannins and Polyphenols Related to Tannins. Molecules. 2011; 16 : 2191-2217.

Apriasari ML (b), Adhani R, Savitri D. Uji sitotoksisitas ekstrak metanol batang pisang Mauli (Musa Sp) terhadap sel fibroblas BHK (Baby hamster kidney) 21. Jurnal Kedokteran Gigi Dentino. 2014; II(2) : 210-214.

Das N, Goshwami D, Hasan S, Raihan SZ. Evaluation of acute and subacute toxicity induced by methanol extract of Terminalia citrine leaves in Sprague Dawley rats. J Acute Dis 2015. Available from: URL: http://dx.doi.org/10.1016/j.joad. Accessed Desember 24, 2015.

Albert B, Johnson A, Lewis J, Raff M, Robert K, Walter P. Molecular Biology of The Cell Fifth edition. United States of Amerika : Garland Science. 2008; p. 928-930.

Halim D, Murti H, Sandra F, Boediono A, Djuwantono T, Setiawan B. Stem cell dasar teori dan aplikasi klinis. Jakarta : Penerbit Erlangga. 2010; hlm.8.

Freshney R. Culture Animal cell : A Manual of Basic Technique and Specialized Application.

th ed. New Jersey : John Wiley and Sons, Inc. 2010; p. 187-194.

Ashok PK and Upadhayaya K. Tannins are astringents. Journal of Pharmacognosy and Phytochemistry. 2012; 1(3) : 45-9.

Ferguson LR. Role of plant polyphenols in genomic stability. Mutation Research. 2001; 75-89.

Andrade RG, Dalvi LT, Silva JMC, Lopes GKB, Alonso A, Hermes-Lima M. The antioxidant effect of tannic acid on the in vitro copper-mediated formation of free radicals. Archive of Biochemistry and Biophysics. 2005; 437 (1).

Gordon, M.H. The mechanism of antioxidants action in vitro. dalam B.J.F. Hudson, editor. Food Antioxidants. Elsivier Applied Science. London. 1990.

Riley PA. Free radicals in biology: oxidative stress and the effects of ionizing radiation. International Journal of Radiation Biology. 1994; 65 (1) : 27–33.

Hagdoost S. Biomarkers of oxidative stress and their application for assessment of individual radiosensitivity. Department of genetic, microbiology and toxicology. Stockholm. 2005.

Jaganathan SK and Mandal M. Involvement of non-protein thiols, mitochondrial dysfunction, reactive oxygen species and p53 in honey-induced apoptosis. Invest New Drugs DOI 10.1007/s10637-009-9302-0. 2009.

Chi Z, Ma X, Cui G, Li M, Li F. Cinnamtannin B-1 Regulates Cell Proliferation of Spinal Cord Astrocytes and Protects the Cell from Oxygen-Glucose-Serum Deprivation/ Reoxygenation-Induced Apoptosis. International Journal of Molecular Sciences. 2013; 14: 15827-15837.

Mitchell RN, Kumar V, Abbas AK, and Fausto N. Buku saku dasar patologis penyakit. Edisi 7. Penerbit EGC. 2006; hlm. 2-28.

Yang J, Tan H, Zhai H, Wang Q, Zhao N, Cai Y, Li M, Zhou H. Research on chemical composition and ensiling characteristics of banana stems and leaves. Adv Mater Res. 2012; 4347–4353:1647–1651.

Ito Y, Pandey P, Place A, Sporn MB, Gribble GW, Honda T, Kharbanda S, Kufe D. The Novel Triterpenoid 2-Cyano-3,12-dioxoolean-1,9-dien-28-oic Acid Induces Apoptosis of Human Myeloid Leukemia Cells by a Caspase-8-dependent Mechanism. Cell Growth & Differentiation. 2000; 11: 261–267.

Taher M, Majid FAA, Sarmidi MR. A proanthocyanidin from Cinnamomum zeylanicum stimulates phosphorylation of insulin reseptor in 3T3-L1 adipocytes. Jurnal Teknologi. 2006; 44(F): 53–68.


Article Metrics

Abstract view : 85 times
PDF - 84 times

Refbacks

  • There are currently no refbacks.


 

Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia
Telp/Fax. (0511) 3255444;

E-mail. fkg@ulm.ac.id / dentino.ulm@gmail.com
Website. fkg.ulm.ac.id

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.