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TOXICITY AND HISTOLOGICAL ANALYSIS OF PINEAPPLE CORE EXTRACT (Ananas comosus) IN MALE WISTAR RATS
Abstract
Bakground: Pineapple core (Ananas comosus) contains bromelain enzyme, which is known to remove dead skin tissue and promote the formation of new tissue. Pineapple fruit and core are also rich in bioactive compounds with potential pharmacological effects. In the oral cavity, it is beneficial in increasing salivary flow. Objective: This study aimed to analyze the toxicity and histological effects of pineapple core extract (Ananas comosus) in male Wistar rats (Rattus norvegicus) using control and treatment groups. Methods: This experimental study used 30 male Wistar rats (Rattus norvegicus), which were administered pineapple core extract supplementation for 14 days. The feed formulation contained pineapple core extract at concentrations of 75% and 90%, with doses ranging from 5,000 mg/kg body weight (BW) to 10,000 mg/kg BW. Histological examination of rat liver tissue was performed at the end of the study. Results: Observation of the control group demonstrated normal liver histology, in which hepatocytes were radially arranged within the hepatic lobules. Similar findings were observed in treatment group (K1), where most hepatocytes appeared normal, with only mild degeneration and necrosis, as well as congestion in the sinusoidal area. Conclusion: Pineapple core extract did not demonstrate toxic effects up to a dose of 10,000 mg/kg BW, although minimal histological alterations in liver tissue were observed.
Keywords: Histology; Male wistar rats; Pineapple corextract (Ananas comosus); Toxicity
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Pramitaningastuti AS, Anggraeny N. Uji Efektifitas Antiinflamasi Ekstrak Etanol Daun Srikaya (Annona squamosa L.) Terhadap Udema Kaki Tikus Putih Jantan Galur Wistar. Jurnal Ilmiah Farmasi. 2017;13(1):8–13.
Putu N, Sari R, Bodhi W, Lebang JS. Anti Inflammatory Activity Test of Ethanol Extract of Papaya Leaf ( Carica Papaya L.) in White Male Rats. Pharmacon. 2021;3:985–93.
Yuliyanda Pardilawati C, Oktarina D, Wulandari S. Uji Efek Analgesik Ekstrak Bonggol Nanas ( Ananas comosus L.) pada Mencit Jantan yang Diinduksi Asam Asetat. Jurnal Farmasi. 2023;1(1):28–33.
Grenvilco O, Kumontoy D, Deeng D, Mulianti T. Pemanfaatan Tanaman Herbal Sebagai Obat Tradisional Untuk Kesehatan Masyarakat di Desan Guaan Kecamatan Mooat Kabupaten Bolang Mongondow Timuw. Holistik. 2023;16(3):1–16.
Juariah S, Wati D. Efektifitas Ekstrak Bonggol Nanas (Ananas comosus L. Merr) terhadap Escherichia coli. Meditory. 2020;8(2):95–100.
Hale LP, Chichlowski M, Trinh CT, Greer PK. Dietary Supplementation with Fresh Pineapple Juice Decreases Inflammation and Colonic Neoplasia in IL-10-deficient Mice with Colitis. NIH. 2011 Dec;16(12):2012–21.
Cervo MMC, Llido LO, Barrios EB, Panlasigui LN. Effects of canned pineapple consumption on nutritional status, immunomodulation, and physical health of selected school children. J Nutr Metab. 2014;1–9.
Easter RL. Pengaruh Pemberian Sari Batang Nanas (Ananas comosus) terhadap Jumlah Trombosit Tikus Wistar yang diberi Paparan Asap Rokok. Diponegoro. 2015;34–9.
Makiyah A. Uji Toksisitas Akut yang diukur dengan Penentuan LD50 Ekstrak Etanol Umbi Iles (Amorphophallus variabilis Bl.) pada Tikus Putih Strain Wistar. MKB. 2017;49(3):145–53.
Annisa T, Sitasiwi AJ, Isdadiyanto S, Jannah SN. Studi Histopatologi Ren Tikus Putih (Rattus Norvegicus L.) Diabetes Setelah Pemberian Cuka dari Kulit Nanas (Ananas Comosus (L.) Mer.). Jurnal Sain Veteriner. 2021 Dec 1;39(3):256.
Meilandani S, Makiyah SN. Proliferasi Limfosit Mencit BALB/c setelah Pemberian Ubi Jalar Ungu (Ipomoea batatas L.) Induced with Ovalbumin. Mutiara Medika. 2015;15(2):83–8.
Salsabila S. Pemanfaatan Ekstrak Bonggol Nanas (Ananas comosus) terhadap Pertumbuhan dan Stimulasi Laju Aliran Saliva pada Tikus Wistar Jantan yang Mengalami Stunting. 2023;31–5.
Apwina TD. Analisis Nutrisi, Fitokimia, dan Aktivitas Spesifik Enzim Bromelin Sebagai Potensi Proteolitik dari Ekstrak Bonggol Nanas (Ananas comosus) Aceh Tengah. 2023;29–35.
Utami YP, Halim Umar A, Syahruni R, Kadullah I. Standardisasi Simplisia dan Ekstrak Etanol Daun Leilem (Clerodendrum minahassae Teisjm. & Binn.). Vol. 2, Journal of Pharmaceutical and Medicinal Sciences. 2017.
Sunny MA, Christijanti W. Efek Ekstrak Daun Teh Hijau (Camelia sinensis L) Terhadap Struktur Histologis Hepar Tikus Diinduksi Parasetamol. UNS. 2023;1:178–82.
Fitri RM, Lubis MS, Dalimunthe GI, Yuniarti R. Skrining Fitokimia, Formulasi dan Uji Mutu Fisik Nanoserum Ekstrak Bonggol Nanas (Ananas comosus (L.) Merr). Journal of Pharmaceutical and Sciences. 2023;6(3):1346–55.
Zahira Ahmad M, Sari Lubis M, Indrayani Dalimunthe G, Rani Z, Farmasi Universitas Muslim Nusantara Al Washliyah F, Jend Ahmad Yani Km J, et al. Formulasi Dan Karakteristik Fisik Krim Nano Ekstrak Bonggol Nanas (Ananas comosus (L) Merr) sebagai Anti-Aging Formulation And Physical Characteristics Cream Of Nano Extract From Pineapple Hump (Ananas comosus (L) Merr) as Anti-Aging. 2024;
Diina TR, Wahyuni IS, Levita J. Toksisitas Akut Dermal Ekstrak Tanaman Sebagai Bagian dalam Aspek Keamanan Obat Herbal Terstandar Topikal. Jurnal Sains Farmasi & Klinis. 2022 Oct 24;9(2):71.
Oktafia N, Susanti R, Purwanti NU. Uji Toksisitas Akut Ekstrak Etanol Kulit Buah Nanas (Ananas comosus L.) Terhadap Tikus Betina Galur Wistar. 2022;1–12.
Yenice G, Iskender H, Dokumacioglu E, Kaynar O, Kaya A, Hayirli A, et al. Dietary bromelain supplementation for improving laying performance, egg quality and antioxidant status. European Poultry Science. 2019;83:1–16.
Salasa AM. Aktivitas Ekstrak Kulit Buah Nanas (Ananas comosus L.) Terhadap Pertumbuhan Pseudomonas aeruginosa. Media Farmasi. 2019 May 25;13(2):1.
Fitrya F, Elfita E, Ferlinahayati, Septami SF, Sabrina UT, Wahyuni FS, et al. Safety evaluation of ethanol extract from Artocarpus altilis leaves as a herbal medicine candidate: Studies in acute and subchronic toxicity. Phytomedicine Plus. 2025 Feb;5(1):100696.
Ma ZT, Shi Z, Xiao XH, Wang JB. New Insights into Herb-Induced Liver Injury. Antioxid Redox Signal. 2023 Jun 1;38(16–18):1138–49.
Liao Y, Lv F, Quan T, Wang C, Li J. Flavonoids in natural products for the therapy of liver diseases: progress and future opportunities. Front Pharmacol. 2024 Oct 24;15.
Kumar V, Mangla B, Javed S, Ahsan W, Kumar P, Garg V, et al. Bromelain: a review of its mechanisms, pharmacological effects and potential applications. Food Funct. 2023;14(18):8101–28.
Mudiana W, Gusti I, Sudisma N, Luh N, Setiasih E, Sudira W. Gambaran Histologi Hati Tikus Putih (Rattus norvegicus) yang Diberikan Ekstrak Bunga Kecubung (Datura metel L.) Sebagai Anestesi. 2023;
Juriah S, Wati D. Ekstrak Bonggol Nanas (Ananas comosus L. Merr) Terhadap Escherichia coli. Meditory. 2020;8(2):95–100.
Hu PA, Chen CH, Guo BC, Kou YR, Lee TS. Bromelain Confers Protection Against the Non-Alcoholic Fatty Liver Disease in Male C57BL/6 Mice. Nutrients. 2020 May 18;12(5):1458.
Yantih N, Harahap Y, Sumaryono W, Setiabudy R, Rahayu L. Hepatoprotective activity of pineapple (Ananas comosus) juice on isoniazid-induced rats. Journal of Biological Sciences. 2017;17(8):388–93.
DOI: http://dx.doi.org/10.20527/dentino.v11i2.26339
DOI (PDF): http://dx.doi.org/10.20527/dentino.v11i2.26339.g12524
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