TOXICITY TEST OF DAYAK ONION BULBS EXTRACT (Eleuthherine palmifolia (L) Merr) ON Artemia salina LEACH USING BSLT METHOD (Preface Study As Root Canal Irrigation Materials)

Khairunnisa Khairunnisa, Muhammad Yanuar Ichrom Nahzi, Sherli Diana

Abstract


Background: Dayak onion (Eleutherine palmifolia (L) Merr) is a native plant from Central Kalimantan, which contains active compounds as anti-bacterials that can be used as an alternative material for root canal irrigation. High toxicity is one of the causes of root canal failure, so it takes a toxicity test to determine the toxic effects of the dayak onion bulbs extract. Purpose: To analyze the difference of toxic effects with various concentrations of dayak onion bulbs extract on Artemia salina Leach using BSLT method. Material and Methods: This study used true experimental research design, namely post-test only with control group design treated with 11 different treatments, which were dayak onion bulbs extract with concentrations 10 mg/ml, 20 mg/ml, 30 mg/ml, 40 mg/ml, 50 mg/ml, 60 mg/ml, 70 mg/ml, 80 mg/ml, 90 mg/ml, 100 mg/ml and sea water as its negative control, and done with 3 times repetitions. Result: The result of the probit analysis in the amount of LC50 was 70,371 mg/ml. The data analysis used Shapiro-Wilk and Levene’s Test results p > 0,05 means that all the data was normally distributed and homogenous. Further test with One Way Anova obtained result p < 0,05 which means there was a significant difference in the number of dead larvae between concentrations. Further test with Pos-hoc LSD obtained result that there was a significant difference at concentration 100 mg/ml with 10 mg/ml with value p=0,001. Conclusion: The highest toxic effect on dayak onion bulbs extract on Artemia salina Leach is at concentration of 100 mg/ml.

Keywords


Toxicity Test; Dayak Onion; Artemia salina Leach; BSLT

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