PHYTOCHEMICAL AND CYTOTOXICITY TESTING OF RAMANIA LEAVES (Bouea macrophylla Griffith) ETHANOL EXTRACT TOWARD VERO CELLS USING MTT ASSAY METHOD (Preliminary study of adjuvant therapy materials to the preparation of the drug)

Laila Fitri, Irham Taufiqurrahman, Irnamanda DH

Abstract


Background: Ramania leaves (Bouea macrophylla Griffith) are one of the local medicinal plants of South Kalimantan which is used as adjuvant therapy material. Previous research showed that ramania leaves ethanol extract showed that the extract has potential as adjuvant therapy material for anticancer, but the safety of this material is not yet known so it  needs to be tested with cytotoxicity test to know the toxic properties of the material toward normal cells in vitro. Objective: This research aims to determine the cytotoxicity properties of ramania leaves ethanol extract toward Vero cells using MTT assay method. Method: It was a true experimental research using posttest-only with control group design consist of 8 treatment groups: 31.25μg/mL, 62.5μg/mL, 125μg/mL, 250μg/mL, 500μg/mL, 1000μg/mL, 2000μg/mL and 4000μg/mL and 2 control groups which were cell control and media control. Study phases include phytochemical test, tannic acid test, cell culture, harvest and cell calculation and cytotoxicity test. The result test was read by ELISA reader, the absorbance will be calculated  by a  formula. Results: The test result showed that ramania leaves ethanol extract contained secondary metabolite such as phenol, flavonoid, steroid and terpenoid. The identification test of tannic acid compound  using TLC method (thin layer chromatography) showed the presence of tannic acid compound.The probit analysis showed IC50in 35.808μg/mL. Conclusion: Based on the research, it can be concluded that ramania leaves ethanol extract are found cytotoxic toward Vero cells after the analysis using MTT assay method (IC50<100μg/mL).

Keywords


ramania leaves ethanol extract; Vero cells; cytotoxicity test; MTT assay; phytochemicals.

Full Text:

PDF

References


Dewi GAT dan Hendrati LY. Analisis Risiko Kanker Payudara Berdasar Riwayat Pemakaian Kontrasepsi Hormonal dan usia Menarche. Jurnal Berkala Epidemiologi. 2015. 3(1): 12–23.

Riset Kesehatan Dasar (Riskesdas). Badan Penelitian dan Pengembangan Kesehatan Departemen Kesehatan RI. Jakarta. 2013. hlm.83-87.

Sirait AM. Faktor Resiko Tumor/Kanker Rongga Mulut dan Tenggorokan di Indonesia Analisis Riskesdas 2007). Media Litbangkes. 2013. 23(3): 123-29.

Syahdana NL, Taufiqurrahman I, Widyamala, E. Uji Efektivitas Ekstrak Etanol Daun Binjai (Mangifera caesia) terhadap Mortalitas Larva Artemia salina Leach. Dentin (Jur. Ked. Gigi) 2017. 1(1): 39-44.

Achmad H, Supriatno, Marhamah, Rosmidar. Aktivitas Antikanker dan Antiproliferasi Fraksi Etanol Sarang Samut (Myrmecodya pendans) Pada Sel Kanker Lidah Manusia SP-C1. Dentofasial. 2014. 13(1): 1-6.

Mardiyaningsih A. dan Nur I. Aktivitas Sitotoksisitas Ekstrak Etanolik Daun Alpukat (Persea americana Mill.) pada Sel Kanker Leher Rahim HeLa. Trad Med J. 2014. 19(1): 24-26.

Aqiila GR, Taufiqurrahman I, Wydiamala E. Uji Efektivitas Ekstrak Etanol Daun Ramania (Bouea macrophylla Griffith) terhadap Mortalitas Larva Artemia salina Leach. Dentino (Jur. Ked. Gigi). 2017. 2(2): 171–76.

Triatmoko B, Hertiani T, Yuswanto A. Sitotoksisitas Minyak Mesoyi (Cryptocarya massoy) terhadap Sel Vero. e-Jurnal Pustaka Kesehatan. 2016. 4(2): 263-66.

Rahmawati E, Sukardiman, Muti, AF. Aktivitas Antikanker Ekstrak n-Heksana dan Ekstrak Metanol Herba Pacar Air (Impatiens balsamina Linn) terhadap Sel Kanker Payudara T47D. Media Farmasi. 2013. 10(2): 47-55.

Cancer Chemoprevention Research Center Fakultas Farmasi UGM (CCRC). Prosedur Tetap Uji Sitotoksik Metode MTT. http://ccrc.farmasi.ugm.ac.id/wp.../03.010.02-uji-sitotoksik-MTT.pdf diakses 22 Mei 2017.

DepKes RI. Farmakope Herbal Indonesia (Edisi 1). Jakarta: Departemen Kesehatan Republik Indonesia. 2008. hlm.175.

Rollando. Aktivitas Sitotoksik Ekstrak dan Fraksi Hasil Fermentasi Fungi Endofit Genus Cephalosporium sp. Diisolasi dari Daun Meniran (Phyllantus niruri Linn.). Jurnal Wiyata. 2016. 3(1): 5-10.

Lisangan MM. Ekstrak Daun Rumput Kebar (Biophytum petersianum) Sebagai Antikapang Aspergillus flavus Toksigen dan Antiaflatoksin Serta Aplikasinya pada Sistem Pangan. Bogor : Sekolah Pascasarjana Institut Pertanian Bogor. Disertasi. 2014. hlm.80.

Vijayarathna S, Sasidharan S. Cytotoxicity of Methanol Extracts of Elaeis Guineensis on MCF-7 and Vero Cell Lines. Elsevier : Asian Pacific Journal of Tropical Biomedicine. 2012. 2(10): 826-29.

Harsini H dan Hertama AFN. Pengaruh Variasi Konsentrasi Ekstrak Kulit Batang Jambu Mete Terhadap Sitotoksitas Sel Fibroblas. Majalah Kedokteran Gigi Indonesia. 2016. 2(1): 6-12.

Emilda Y, Budipratama E, Kuntari S. Uji toksisitas ekstrak bawang putih (Allium Sativum) terhadap Kultur Sel Fibroblas. Dental Journal : Majalah Kedokteran Gigi. 2014. 47(4) : 215-19.

Booth BW, Inskeep BD, Shah H, Park JP, Hay EJ, Burg KL. Tannic Acid Preferentially Targets Estrogen Receptor-Positive Breast Cancer. International Journal of Breast Cancer. 2013. (2013): 1-9.

Mcllwain DR, Berger T, Mak TW. Caspase function in cell death and disease. Cold Spring Harb Perspect Biol. 2013. 5(4): 1-19.

Matsuo M, Sasaki N, Saga K, Kaneko T. Cytotoxicity of Flavonoids toward Cultured Normal Human Cells. Biol. Pharm. Bull. 2005. 28(2): 253-59.

Gunawan CK, Mulawarmanti D, Laihad FM. Sitotoksisitas Ekstrak Daun Avicennia marina terhadap Sel Fibroblas. Denta : Jur. Ked. Gigi. 2014. 8(2): 67-78.

Ke Y, Xu X, Wu S, Huang J, Geng Y, Misra, H, et al. Protective Effects of Extracts from Fructus rhodomyrti against Oxidative DNA Damage In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity. 2013. 2013(9): 1-8.

Berawi KN, Agverianti T. Efek Aktivitas Fisik Proses Pembentukan Radikal Bebas sebagai Faktor Risiko Aterosklerosis. Mojority. 2017. 6(2): 85-90.

Zalukhu ML, Phyma AR, Pizon RT. Tinjauan Pustaka : Proses Menua, Stres Oksidatif dan Peran Antioksidan. CDK-245. 43(10): 733-36.

Kumar V, Abbas AK, Aster JC. Buku Ajar Patologi Robbins. Edisi 9. Singapura: Elsevier Saunders. 2015. hlm.19-20.

Ramadani. Senyawa Bahan Alam Terpenoid. Jurnal Tarbawi. 2016. 1(1): 3-5.

Usman H. Kimia Organik Alam Laut. Makasar : Universitas Hasanuddin. 2014. hlm.27-56.


Article Metrics

Abstract view : 103 times
PDF - 140 times

Refbacks

  • There are currently no refbacks.


 

Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia
Telp/Fax. (0511) 3255444;

E-mail. fkg@ulm.ac.id / dentino.ulm@gmail.com
Website. fkg.ulm.ac.id

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.