THE INFLUENCE OF TOMAN FISH (Channa micropeltes) EXTRACT ON THE NUMBER OF NEOVASCULAR IN DIABETES MELLITUS WOUND HEALING In Vivo Study on the Back of Male Wistar Rat (Rattus novergicus)

Alya Royyana, Amy Nindia Carabelly, Didit Aspriyanto

Abstract


Background: Patients with diabetes mellitus (DM) have delayed wound healing. This happens because
of the depletion of neovascular number. Neovascular plays an important role in wound healing by restoring
blood flow to the wound area, so the new tissue gets enough oxygen and nutrition supply to proliferate. Toman
fish (Channamicropeltes) contains albumin that can increase and decrease the number of neovascular, so it’s
potentially become an alternative medicine to accelerates wound healing in DM. Purpose: The aim of this
research to prove the effect of toman extract dose 16 ml/Kg BW rat orally on the number of neovascular in
wound of diabetic wistar rats on the 4th,8th and 14th day. Materials and methods: This research used true
experimental method with posttest only control group design. This research used 36 wistar rats which were then
divided into 9 groups, toman fish extract dose 16 ml/Kg BW of rat group, haruan fish extract dose 13,54 ml/Kg
BW of rat group and BR2 feed only group. Research result: The results of One Way-Anova and Post Hoc Least
Significant Difference tests showed there was a significant difference between group of toman extract and group
of feed only, while on group of toman extract and group of haruan extract showed there was no significant
difference on 4th,8th and 14th day. The average value of toman extract-given group on 4th, 8thand 14th day were
14,75. 7,5 and 6,75 respectively. Conclusion: Toman extract has effects which are increasing the number of
neovascular on 4th day and decreasing the number of neovascular on 8th and 14th day in wound healing with
DM.

Keywords


Diabetes mellitus, Toman fish extract, Neovascular, Wound healing, Wistar rats.

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References


American Diabetes Association. Diagnosis and Classification of Diabetes Mellitus.Diabetes Care. 2014; 37(1): S81-S90.

Walukow W.G. Gambaran Xerostomia pada Penderita Diabetes Melitus Tipe 2 di Poliklinik Endokrin RSUP Prof. dr. R. D. Kandou Manado. Jurnal e-Gigi. 2013; 7(22): 1-5.

Panchbhai A. Oral Considerations in Diabetes Mellitus. InAvid Science.Recent Advances in Diabetes Treatment.India: Avid Science; 2015. p. 22-31.

Indrasari S.D. Hubungan antara Diabetes Melitus dengan Penyakit Periodontal. Cermin Dunia Kedokteran. 2014; 40(11): 868-869.

Forouhi N.G dan Wareham N.J. Epidemiology of Diabetes. Medicine (Abingdon). 2014; 42(12): 9.

Kementrian Kesehatan RI. Riset Kesehatan Dasar (RISKESDAS). Jakarta: Balitbang Kemenkes RI; 2013. Hlm. 89.

Mansur A.R. Merawat Luka Komplikasi Diabetes. Dalam Hafid, editor. Majalah Kesehatan Muslim Diabetes Mellitus, edisi ke-9. Yogyakarta: Pustaka Muslim; 2013. Hlm. 31.

Sura G.M, Carabelly A.N, Apriasari M.L. Aplikasi Ekstrak Haruan (Channa striata) 100% pada Luka Punggung Mencit (Mus musculus) terhadap Jumlah Neutrofil dan Makrofag. Jurnal PDGI. 2013; 62(2): 41-44.

Irwanda W.F, Andrie M, Luliana S. Uji Efek Penyembuhan Luka Fase Air Ekstrak Ikan Toman (Channa micropeltes) pada Tikus Putih Jantan Wistar yang Diberi Luka Sayat. Jurnal UNTAN. 2015; 3(1): 1,11.

Muslim dan Syaifudin M. Domestikasi Calon Induk Ikan Gabus (Channa striata) dalam Lingkungan Budidaya (Kolam Beton). Majalah Ilmiah Sriwijaya. 2012; 21(1): 20-27.

Nicodemus, AndrieM, LulianaS. Uji Efek Penyembuhan Luka Sayat EkstrakIkanToman (Channa micropeltes) secara Oral pada Tikus Putih Jantan Wistar. Jurnal UNTAN. 2014; 1(1): 12.

Firlianty, Supriyatno E, Nursyam H, Hardoko, Mustafa A. Chemical Composition and Amino Acid Profile of Channidae Collected From Central Kalimantan, Indonesia. International Journal of Science and Technology (IJSTE). 2013; 2(4): 25-29.

Suhartono E. Toksisitas Oksigen Reaktif & Antioksidan di Bidang Kedokteran dan Kesehatan. Yogyakarta: Gosyen Publishing; 2016. Hlm. 81.

14. Marjiyanto, Murtutik L, Suwarni A. Hubungan Kadar Albumin dengan Penyembuhan Luka pada Pasien Post Operasi Laparatomy di Ruang Mawar Rumah Sakit Slamet Riyadi Surakarta. Jurnal Ilmu Keperawatan Indonesia. 2013; 1(1): 80-97.

Ponrasu T, Subamekala M.T, Ganeshkumar M, Suguna L. Role of Annona squamosa on antioxidants during wound healing in streptozotocin nicotinamide induced diabetic rats. Journal of Pharmacognosy and Phytochemistry. 2013; 2(4): 77-84.

16. Tchaikovski V, Waltenberger J. Angiogenesis and Arteriogenesis in Diabetes Mellitus: Signal Transduction Defects as The Molecular Basis of Vascular Cell Dysfunction. In Elisabeth D, Christian K, editors. Therapeutic Neovascularization – Quo vadis?. Berlin: Springer-Heidelberg; 2007. p. 52.

17. Ponugoti B, Xu F, Zhang C, Tian C, Pacios S, Graves D.T. FOXO1 promotes wound healing through the up-regulation of TGF-beta1 and prevention of oxidative stress. J Cell Biol. 2013; 203(2):327–43.

18. Ullah A, Khan A, Khan I. Diabetes Mellitus and Oxidative Stress- A Concise Review. Saudi Pharmaceutical Journal. 2016; 24(5): 547-553.

19. Kupsco A, Schlenk D. Oxidative Stress, Unfolded Protein Response, and Apoptosis in Developmental Toxicity. Int Rev Cell Mol Biol. 2015; 317(1): 3.

20. Ciorba A, Chicca M, Bianchini C, Aimoni C, Pastore, A. Review: Sensorineural hearing loss and endothelial dysfunction due to oxidative stress: Is there a connection?. The Journal International of Advanced Otology.2012; 8(1): 16-20.

Johnson K.E, Wilgus T.A. Vascular Endothelial Growth Factor and Angiogenesis in the Regulation of Cutaneous Wound Repair. Advances in Wound Care.2014; 3(10):647-658.

Tahegorabi Z, Khazaei M. Imbalance of Angiogenesis in Diabetic Complications: The Mechanism. Int J Prev Med. 2012;3(12):832.

Suhartono E, Djati M.S. Radikal Bebas dan Intoksikasi Kadmium. Banjarmasin: Pustaka Banua; 2014. Hlm. 7-21.

Lord R.S, Bralley J.A. Laboratory Evaluations for Integrative and Functional Medicine. Dulith: Metametrix Institute; 2008. p. 520.

Halliwell B, Gutteridge J. Free Radicals in Biology and Medicine, Ed 4th. New York: Oxford University Press; 2007. p. 138-143.

Pathak K.V, Bellamri M, Wang Y, Langouët S, Turesky R.J. 2-Amino-9H-pyrido[2,3-b] indole (AC) Adducts and Thiol Oxidation of Serum Albumin as Potential Biomarkers of Tobacco Smoke. The Journal of Biological Chemistry. 2015; 290(26): 16306 –16307, 16316.

Clyne A.M. Reactive Oxygen Species in Physiologic and Pathologic Angiogenesis. In Cynthia Reinhart-King, editor. Mechanical and Chemical Signaling in Angiogenesis. Berlin: Springer-Heidelberg; 2013. p. 71-81.

Prasetyono T.O, Saputra D.K.A. Panduan Klinis Manajemen Luka. Jakarta: EGC; 2016. Hlm. 3-7.

Rahmawati G, Rachmawati F.N, Winarsi H. Aktivitas Superoksida Dismutase Tikus Diabetes Yang Diberi Ekstrak Batang Kapulaga Dan Glibenklamid. Scripta Biologica. 2014; 1(3): 198.

Hansen U.K. Human serum Albumin: A Multifunctional Protein. In Masaki Otagiridan Victor Tuan Giam Chuang, editors. Albumin in Medicine: Pathological and Clinical Applications. Singapore: Springer; 2016. p.19.

Dunnill C, Patton T, Brennan J, Barrett J, Dryden M, Cooke J, et al. Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS-modulating technologies for augmentation of the healing process. International Wound Journal. 2015; 14(1): 89–96.

Zhou Y, Yan H, Guo M, Zhu J, Xiao Q, Zhang L. Review Article: Reactive Oxygen Species in Vascular Formation and Development. Hindawi Publishing Corporation. 2013; 2013(1): 1-2, 5-6.

Bali J, Bali R.T. Pathological ocular angiogenesis in diabetes: A perspective of emerging paradigms and current evidence. Journal of Clinical Ophthalmology and Research. 2012; 1(1): 3-10.

Apriasari M.L, Dachlan Y.P, Ernawati D.S. Potensi Bahan Alam Terhadap Penyembuhan Ulser Mukosa Mulut. Jakarta: Salemba Medika; 2017. Hlm. 31, 34, 48-49.

Soepribadi I. Regenerasi dan Penyembuhan. Jakarta: Sagung Seto; 2013. Hlm. 7-11.

Mutiara G, Nurdiana, Utami Y.W. Efektifitas Hidrogel Binahong (Anrederacordifolia (Ten.) Steenis) terhadap Penurunan Jumlah Makrofag pada Penyembuhan Luka Fase Proliferasi Tikus Putih (Rattus norvegicus) Galur Wistar Kondisi Hiperglikemia. Majalah Kesehatan FKUB. 2015; 2(1): 29-40.

Reinke J.M, Sorg H. Wound Repair and Regeneration. European Surgical Research. 2012; 49(1): 35-43.

Suprayitno E. Penggunaan Albumin IkanGabus (Ophiocephalus striatus) Pada Penutupan Luka. Malang: Universitas Brawijaya; 2009. Hlm. 9-23.

Kumar V, Cotran L.S. dan Robbins S.L. Buku Ajar Patologi. Jakarta: EGC; 2007. Hlm. 80-2.

Arisanty I.P. Manajemen Perawatan Luka: Konsep Dasar. Jakarta: EGC; 2013. Hlm. 32-33.

Murdani O.J, Andrie M. dan Taurina W. Uji Efek Penyembuhan Luka Sayat Ekstrak Ikan Toman (Channa micropeltes) Secara Oral Pada Tikus Jantan Galur Wistar yang Diinduksi Streptozotocin. Jurnal UNTAN. 2015; 3(1):1-7.




DOI: http://dx.doi.org/10.20527/dentino.v3i2.5362

DOI (PDF): http://dx.doi.org/10.20527/dentino.v3i2.5362.g4522

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