UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN TUNGGAL DIBANDINGKAN KOMBINASI SEDUHAN DAUN TEH HIJAU (Camellia sinensis) DAN MADU (Studi in Vitro terhadap Jumlah Koloni Bakteri Rongga Mulut) Tinjauan pada Mahasiswa PSKG FK Unlam Banjarmasin Angkatan 2011-2013
Abstract
ABSTRACT
Green tea has various active substances; one of them is polyphenol, mostly cathechin, an active compound which can protect teeth from caries because of its anti-streptococcal activity. A high content of minerals in honey has alkali characteristic thus the disinfectant trait in oral cavity. The aim of this study was to assess whether single and combined preparations of brewed green tea and honey can decrease bacterial colony count in oral cavity. This study was quasi experimental with pretest and posttest controlled group design. Samples of 38 Students of Dentistry Study Program Universitas lambung Mangkurat were divided into 19 groups: 3 groups were given 25%, 50%, 100% green tea in single preparations, 4 groups were given 6,25%, 12,5%, 25%, 50% honey in single preparations, and 12 groups were given combined preparations of green tea and honey. Bacterial colony before and after gargling grown on isolated medium was counted using colony counter. Repeated anova test showed p value of 0,037 (p < 0,05), stating that there was a significant difference among treatment groups. In conclusion, combined preparations of 50% green tea + 12,5% honey and 100% green tea + 25% honey were more effective in decreasing bacterial colony count.
ABSTRAK
Teh hijau mempunyai beberapa komponen aktif yang salah satunya adalah polifenol berupa katekin, suatu senyawa aktif dalam melindungi gigi dari karies karena memiliki aktivitas anti-streptococcal. Kandungan mineral yang tinggi pada madu mempunyai sifat basa (mengandung unsur alkali) sehingga dapat berfungsi sebagai desinfektan terhadap rongga mulut. Penelitian ini bertujuan untuk mengetahui apakah sediaan tunggal dan kombinasi seduhan daun teh hijau dan madu dapat menurunkan jumlah koloni bakteri rongga mulut. Penelitian ini bersifat eksperimental kuasi dengan rancangan pretest and posttest controlled group design. Sampel sebanyak 38 orang mahasiswa Program Studi Kedokteran Gigi Fakultas Kedokteran yang dibagi dalam 19 kelompok yaitu sebanyak 3 kelompok perlakuan sediaan tunggal teh hijau menggunakan konsentrasi 25%, 50%, dan 100%, sebanyak 4 kelompok perlakuan sediaan tunggal madu menggunakan konsentrasi 6,25%, 12,5%, 25%, dan 50%, sebanyak 12 dengan kelompok perlakuan sediaan kombinasi teh hijau dan madu. Jumlah koloni yang tumbuh pada media isolasi sebelum dan sesudah berkumur dihitung menggunakan alat colony counter. Hasil uji Repeated anova menunjukan nilai p = 0,037 (p < 0,05), terdapat perbedaan yang bermakna diantara perlakuan. Dapat disimpulkan bahwasediaan kombinasi teh hijau 50% dengan madu 12,5% dan teh hijau 100% dan madu 25% lebih efektif dalam menurunkan jumlah koloni bakteri.
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DOI: http://dx.doi.org/10.20527/dentino.v1i2.552
DOI (PDF): http://dx.doi.org/10.20527/dentino.v1i2.552.g466
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