PENGARUH PAPARAN BATUBARA TERHADAP JUMLAH MIKRONUKLEUS MUKOSA BUKAL PADA PEKERJA TAMBANG BATUBARA DI KECAMATAN MURUNG PUDAK KABUPATEN TABALONG
Abstract
ABSTRACT
Background: Continuous exposure of genotoxic substance such as coal dust can cause DNA damage. Micronucleus is DNA damage caused by genotoxic substance that manifest on buccal mucosa cell. Micronucleus is cytoplasmic chromatic mass with round shaped located close to nucleus and microscopically visible. Purpose: The purpose of this study was to identify effect of coal exposure on the number of micronucleus buccal mucosa on coal miner. Methods: The method of this study was analytic observational with cross-sectional approach. The total sample of this study was 60 respondents divided into 2 groups, each group contained 30 respondents. The data was primary data which was the result of swabbed of buccal mucosa epithelial cells. Results: The result showed the average number of buccal mucosa micronucleus on coal miners was 25,83 13,28 and non-coal miner is 11,10 3,45. Data analyzed with T test Independent and obtained significant different on the number of micronucleus between coal miners and non-coal miner (p=0,000). Conclusion: Based on this study can be concluded that coal dust exposure affected on the number of micronucleus buccal mucosa on coal miners in Kecamatan Murung Pudak Kabupaten Tabalong.
ABSTRAK
Latar belakang: Paparan terus menerus dari suatu substansi genotoksik, seperti debu batubara akan menyebabkan suatu kerusakan DNA. Kerusakan DNA akibat zat genotoksik yang dapat dilihat pada sel mukosa bukal adalah mikronukleus. Mikronukleus merupakan massa kromatik sitoplasmik berbentuk bulat/oval terletak dekat dengan nukleus dan tampak secara mikroskopik. Tujuan: Tujuan penelitian ini adalah mengetahui adanya pengaruh paparan batubara terhadap jumlah mikronukleus mukosa bukal pada pekerja tambang batubara di Kecamatan Murung Pudak Kabupaten Tabalong. Metode: Penelitian ini menggunakan metode penelitian observasional analitik dengan pendekatan cross-sectional. Jumlah sampel pada penelitian ini adalah 60 orang dengan tiap-tiap kelompok masing-masing 30 orang. Data yang diperoleh adalah data primer, yaitu hasil apusan sel epitel mukosa bukal. Hasil: Hasil penelitian menunjukkan rata-rata jumlah mikronukleus mukosa bukal pada pekerja tambang batubara adalah 25,83 13,28 dan pada bukan pekerja tambang batubara adalah 11,10 3,45. Data dianalisis menggunakan T test Independent dan didapatkan perbedaan bermakna pada rata-rata jumlah mikronukleus antara kelompok pekerja tambang batubara dan bukan pekerja tambang batubara (p=0,000). Kesimpulan: Berdasarkan penelitian ini dapat disimpulkan bahwa paparan batubara berpengaruh terhadap jumlah mikronukleus mukosa bukal pada pekerja tambang batubara di Kecamatan Murung Pudak Kabupaten Tabalong.
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DOI: http://dx.doi.org/10.20527/dentino.v1i2.557
DOI (PDF): http://dx.doi.org/10.20527/dentino.v1i2.557.g470
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