PENGARUH PAPARAN BATUBARA TERHADAP JUMLAH MIKRONUKLEUS MUKOSA BUKAL PADA PEKERJA TAMBANG BATUBARA DI KECAMATAN MURUNG PUDAK KABUPATEN TABALONG

Renita Renita Rahmad, Nurdiana Dewi, Lena Rosida

Abstract


ABSTRACT

Background: Continuous exposure of genotoxic substance such as coal dust can cause DNA damage. Micronucleus is DNA damage caused by genotoxic substance that manifest on buccal mucosa cell. Micronucleus is cytoplasmic chromatic mass with round shaped located close to nucleus and microscopically visible. Purpose: The purpose of this study was to identify effect of coal exposure on the number of micronucleus buccal mucosa on coal miner. Methods: The method of this study was analytic observational with cross-sectional approach. The total sample of this study was 60 respondents divided into 2 groups, each group contained 30 respondents. The data was primary data which was the result of swabbed of buccal mucosa epithelial cells. Results: The result showed the average number of buccal mucosa micronucleus on coal miners was 25,83  13,28 and non-coal miner is 11,10  3,45. Data analyzed with T test Independent and obtained significant different on the number of micronucleus between coal miners and non-coal miner (p=0,000). Conclusion: Based on this study can be concluded that coal dust exposure affected on the number of micronucleus buccal  mucosa on coal miners in Kecamatan Murung Pudak Kabupaten Tabalong.

 

ABSTRAK

Latar belakang: Paparan terus menerus dari suatu substansi genotoksik, seperti debu batubara akan menyebabkan suatu kerusakan DNA. Kerusakan  DNA akibat zat genotoksik yang dapat dilihat pada sel mukosa bukal adalah mikronukleus. Mikronukleus merupakan massa kromatik sitoplasmik berbentuk bulat/oval terletak dekat dengan nukleus dan tampak secara mikroskopik. Tujuan: Tujuan penelitian ini adalah mengetahui adanya pengaruh paparan batubara terhadap jumlah mikronukleus mukosa bukal pada pekerja tambang batubara di Kecamatan Murung Pudak Kabupaten Tabalong. Metode: Penelitian ini menggunakan metode penelitian observasional analitik dengan pendekatan cross-sectional. Jumlah sampel pada penelitian ini adalah 60 orang dengan tiap-tiap kelompok masing-masing 30 orang. Data yang diperoleh adalah data primer, yaitu hasil apusan sel epitel mukosa bukal.  Hasil: Hasil penelitian menunjukkan rata-rata jumlah mikronukleus mukosa bukal pada pekerja tambang batubara adalah 25,83  13,28 dan pada bukan pekerja tambang batubara adalah 11,10 3,45. Data dianalisis menggunakan T test Independent dan didapatkan perbedaan bermakna pada rata-rata jumlah mikronukleus antara kelompok pekerja tambang batubara dan bukan pekerja tambang batubara (p=0,000). Kesimpulan: Berdasarkan penelitian ini dapat disimpulkan bahwa paparan batubara berpengaruh terhadap jumlah mikronukleus mukosa bukal pada pekerja tambang batubara di Kecamatan Murung Pudak Kabupaten Tabalong.


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References


Devi B, Prayoga D. Mining and development

in Indonesia: An overview of the regulatory

framework and policies. International Mining

for Developmental Centre 2013: 1-67

Sarmadi AS. Penerapan hukum berbasis

hukum progresif pada pertambangan batu bara

di Kalimantan Selatan. Jurnal Hukum 2012;

: 8-19

BPS Kabupaten Tabalong. Tabalong:

Direktorat Jendral Pemberdayaan Masyarakat

Desa dan Departemen Dalam Negeri. 2013; 7-

PT Adaro Energy, Tbk. Laporan bulanan

kegiatan eksplorasi periode Desember 2013

PT Adaro Energy, Tbk. Kalimantan Selatan:

PT Adaro Energy,tbk. 2013

Produksi batubara (PKP2B DAN BUMN).

Jakarta: Ditjen Minerba. 2013

Sumber daya batubara: Tinjuan lengkap

mengenai batu bara. Jakarta:World Coal

Institute. 2009; 2-10

Chen Y, Shah N, Huggins FE, Huffman GP.

Transmission electron microscopy

investigation of ultrafine coal fly ash particles.

Environ Sci Technol 2005; 39: 1144-1151

Zakrzewesky S, Principles of priority

polycyclic aromatic hydrocarbons in complex

mixtures. Mutat Res Genet Toxical Environ

Mutagen. 2002; 515: 85-89

Cawson RA, Odell EW. Oral cancer. 5th ed.

London: Churcill Livingstone. 2000

Moss-Salentijn L and Klyvert M. Dental and

Oral Tissues: An Introduction for

Paraprofessionals in Dentistry. Philadelphia:

Lea & Febiger. 1980; 27-43

Nina H, Claudia B, Micheline K, Stefano B,

Errol Z, Siegfried K. The micronucleus assay

in human buccal cell as a tool for

biomonitoring DNA damage : The HUMN

project perspective on current status and

knowledge gaps. Elsevier, 2008: 16-30

Balakrishnan M, Sellappa S, Palanisamy S.

Assesment of occupational genotoxic risk by

using micronucleus test among cement mill

workers of South India. Journal of Herbal

Science and Technology 2009;24: 26-28

Swarjana IK. Metodologi penelitian

kesehatan: tuntunan praktis pembuatan

proposal penelitian. Yogyakarta : ANDI,

; hal. 102

Gay LR, PL Diehl. Research Methods for

Business and Management. Singapura :

Prentice Hall, 1996; 3: 198-199

Leon-Meija G, Perez LE, Hoyos Giraldo LS,

et al. Assesment of DNA damage in coal

open-cast mining workers using the

cytokinesis-blocked micronucleus test and the

comet assay. Science of the Total

Environment 2011; 409: 686-691

Kashyap B, Reddy PS. Micronuclei assay of

exfoliated oral buccal cells: Means to assess

the nuclear abnormalities in different diseases.

Journal of Cancer Research and Therapeutics

; 8: 184-191Jois HS, Alka DL dan

Mohan K. Micronucleus as Potential

Biomarker of Oral Carcinogenesis. IJDA

;2(2):197-202

Pratheepa SN, Kauer S, Reddy KS et al.

Micronucleus Index: An Early Diagnosis in

Oral Carcinoma. J.Anat. Soc. India 2008; 57:

-13

Naderi NJ, Farhadi S, Sarhsar S.

Micronucleus assay of buccal mucosa cells in

smokers with the history of smoking less and

more than 10 years. Indian Journal of

Pathology and Microbial 2012; 55: 433-438

Rohr P, Kvitko K, Da Silva FR et al. Genetic

and Oxidative Damage of Peripheral Blood

Lymphocytes Inworkers with Occupational

Exposure to Coal. Mutation Research 2013;

: 23-28.




DOI: http://dx.doi.org/10.20527/dentino.v1i2.557

DOI (PDF): http://dx.doi.org/10.20527/dentino.v1i2.557.g470

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