THE EFFECT OF TOPICAL TOMAN (Channa micropeltes) FISH EXTRACT ON THE NUMBER OF NEUTROFIL CELLS IN DIABETES MELITUS WOUND HEALING (In Vivo Study on Male Wistar (Rattus novergicus) Rat’s Back)

Annisa Vara Nurdianty, Maharani Laillyza Apriasari, Amy Nindia Carabelly

Abstract


Backgrounds: Diabetes mellitus forges the wound healing process to last longer. Toman fish which contains albumin and omega-6 fatty acid is proven to to enhance wound healing process in this systemic condition. Patients who suffer from diabetes mellitus retain high neutrophil number. Omega-6 fatty acids contained in Toman fish can decrease the number of  neutrophil cells. Objectives:  To prove the effect of Toman fish (Channa micropeltes) extract at 20% concentration topically on the number of neutrophil in diabetes mellitus-induced Wistar Rat (Rattus novergicus) injuries on day 4, 8 and 14.  Methods: This research was a true experimental study with posttest-only and control group with complete random design. Thirty six male Wistar rats (Rattus novergicus) was divided evenly for three treatments comprised of negative control given BR2 comfeed, positive control treated with Haruan fish (Channa striata) extraxt at 10% concentration topically and treatment group given with Toman fish (Channa micropeltes) extract at 20% concentration topically. Results: The data was analyzed using One Way ANOVA and was presenting the result of the 4th day (p=0.000), 8th day (p=0.001) and 14th day (p=0.000). Post-hoc Least Significant Difference (LSD) showed that p value was less than 0.05 which means that there was a significance difference in the mean of all treatment groups. Conclusion: Toman fish (Channa micropeltes) extract possesses the capacity in decreasing the number of neutrophil on 4th, 8th and 14th day in diabetic wound healing of Wistar Rat (Rattus novergicus).

Keywords


Channa micropeltes; diabetic wound healing; neutrophil cell

Full Text:

PDF

References


Fatimah R.N. Diabetes Melitus Tipe 2. J Majority. 2015; 4(5) : 93-101.

Obradors E.M, Devesa A.E, Salas E.J, Vinas M. Oral manifestations of Diabetes Mellitus. A Systematic Review. Journal section: Medically compromised patients in Dentistry. 2017; 22(5) : 586-594.

IDF. International Diabetes Federation. Atlas Diabetes. Ed. 8th. 2017. 46-79. Karuranga S, Fernandes J.D.R, Huang Y, Malanda B. Available from: https://www.diabete.qc.ca/en/understand-diabetes/resources/getdocumentutile/IDF-DA-8e-EN-finalR3.pdf Cited on 19 Agustus 2018.

Suryani N, Pramono, Septiana H. Diet dan Olahraga Sebagai Upaya Pengendalian Kadar Gula Darah Pada Pasien Diabetes Melitus Tipe 2 di Poliklinik Penyakit Dalam RSUD Ulin Banjarmasin Tahun 2015. Jurkessia. 2016; VI(2) : 1-10.

Audina N, Carabelly A.M, Aspriyanto D. The Effect Of Toman (Channa micropaltes) Fish Extract On Epithelial Thickness In Diabetes Mellitus Wound Healing. Dentino Jurnal Kedokteran Gigi. 2018; 3(1) : 22-28.

Chasanah E, Nurilmala M, Purnamasari A.R, Fithriani D. Komposisi Kimia, Kadar Albumin Dan Bioaktivitas Ekstrak Protein Ikan Gabus (Channa striata) Alam Dan Hasil Budidaya. JPB Kelautan dan Perikanan. 2015; 10(2) : 123 – 132.

Restu. Penurunan Kadar Air Untuk Memperpanjnag Daya Awet Produk Wadi Ikan Patin (Pangasius hypophthalmus). Jurnal Ilmu Hewani Tropika. 2015; 4(1) : 12-15.

Karuniawan A.M, Andrie M, Riza H. Uji Efek Penyembuhan Luka Sayat Salep Ekstrak Ikan Toman (Channa micropeltes) Secara Topikal Pada Tikus Yang Diinduksi STREPTOZOTOCIN. 2016; 1(1) : 1-14.

Fajriani N, Carabelly A.N, Apriasari M.L. The Effect Of Toman Fish Extract (Channa micropeltes) On Neutrophil in Diabetes Mellitus Wound Healing. Dentino Jurnal Kedokteran Gigi. 2018; 3(1): 15-21. Fatimah R.N. Diabetes Melitus Tipe 2. J Majority. 2015; 4(5) : 93-101.

Patterson E, Wall R, Fitzgerald G.F, Ross R.P. Health Implications of High Dietary Omega-6 Polyunsaturated Fatty Acids. Journal of Nutrition and Metabolism. 2012;1-16. Doi:10.1155/2012/539426.

Firlianty, Suprayitno, E, Nursyam H, Hardoko dan Mustafa, A. Chemical Composition And Amino Acid Profile Of Channidae Collected From Central Kalimantan, Indinesia. International Journal Of Science and Technology (IJSTE). 2013; 2 : 25-29.

Nicodemus, Andrie M, Luliana S. Uji Efek Penyembuhan Luka Sayat Ekstrak Ikan Toman (Channa micropeltes) Secara Oral Pada Tikus Putih Jantan Wistar. Jurnal Mahasiswa Farmasi UNTAN. 2014; 1(1) : 14.

Alauddin A, Andrie M, Purwanti N.U. Uji Efek Ekstrak Ikan Gabus (Channa striata) Pada Luka Sayat dengan tikus putih Jantan Galur Wistar yang diberikan Secara Oral. Jurnal Mahasiswa Farmasi UNTAN. 2016; 1(1) : 1-16.

Andrie M, Sihombing D. Efektivitas Sediaan Salep Yang Mengandung Ekstrak Ikan Gabus (Channa striata) Pada Proses Penyembuhan Luka Akut Stadium II Terbuka Pada Tikus Jantan Galur Wistar. Pharm Sci Res. 2017; 4(2) : 88-98.

Apriasari M.L, Puspitasari D. Effect of Channa micropeltes for Increasing Lymphocyte and Fibroblast Cells in Diabetic Wound Healing. Journal of Medical Sciences. 2018; 18(4) : 205-210.

16.Lande’n N.X, Li D, Stahle M. Transition From Inflammation to Proliferation: a Critical Step During Wound Healing. Celuller and Molecular Life Sciences. 2016; 73 : 3861-3865. DOI 10.1007/s00018-016-2268-0.

Firlianty. Vacuum Drying Albumin Powder of Snakehead (Channa micropeltes) Potential for Wound Healing from Central Kalimantan, Indonesia. International Journal of ChemTech Research. 2016; 9(5) : 263-269.

Irwanda W.F, Andrie M, Luliana S. Uji Efek Penyembuhan Luka Fase Air Ekstrak Ikan Toman (Channa micropeltes) Pada Tikus Putih Jantan Wistar yang Diberi Luka Sayat. Jurnal Mahasiswa Farmasi UNTAN. 2015; 1(1 ) : 10.

Ngui W.S.Y, Nur H.H, Nadiah R, Saiful I.Z Malaysia Snakehead Channa Striatus and Micropeltes: Physico-chemical Properties of Fillet Fish Oil and Water-soluble Extract. Chemical Engineering Transactions. 2017; 56 : 61-66.

Selders G.S, Fetz A.E, Radic M.Z, Bowlin G.L. An Oerview of the Role of Neutrophils in Innate Immunity, Inflammation And Host-Biomaterial Integration. Regenerative Biomaterials. 2017; 55–68. doi: 10.1093/rb/rbw041

Darby I.A, Laverdet B, Bonte F, Desmouliere A. Fibroblasts and Myofibroblasts in Wound Healing. Clinical, Cosmetic and Investigational Dermatology. 2014; 7: 301 -303.

Sumbayak E.M. Fibroblas: Struktur dan Peranannya dalam Penyembuhan Luka. Jurnal Histologi FK UKRIDA. 2016; 1(2) : 1-6.

Apriasari M.L, Dachlan, Y.P, Ermawati, D.S. Potensi Bahan Alam Terhadap Penyembuhan Ulser Mukosa Mulut. Salemba Medika. Jakarta. 2017. p. 28-31.

Kumar V, Abbas A.K, Aster J.C. Robbins Basic Pathology. 9th Ed. Elsevier Saunders, Philadelphia. 2013. p. 75-100.

Murdani O.J, Andrie M, Taurina W. Uji Efek Penyembuhan Luka Sayat Ekstrak Ikan Toman (Channa Micropeltes) Secara Oral Pada Tikus Jantan Galur Wistar Yang Diinduksi treptozotocin. Jurnal Mahasiswa Farmasi UNTAN. 2016; 1(1): 1-2.


Article Metrics

Abstract view : 0 times
PDF - 0 times

Refbacks

  • There are currently no refbacks.


 

Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia
Telp/Fax. (0511) 3255444;

E-mail. fkg@ulm.ac.id / dentino.ulm@gmail.com
Website. fkg.ulm.ac.id

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.