THE EFFECT OF FLAVONOID PROPOLIS KELULUT (Trigona spp) EXTRACT ON MACROPHAGE CELL NUMBER IN PERIODONTITIS (IN VIVO STUDY IN MALE WISTAR RATE (Rattus novergicus) GINGIVA)

Makfiyah Saidah, Beta Widya Oktiani, Irham Taufiqurrahman

Abstract


Background : Periodontitis is a condition where there is an increase in the number of inflammatory cells, namely macrophages in periodontal tissue. Macrophag cell is 12-15μm in oval shape cell with purplish blue cytoplasm and this cell’s function is to phagocytes bacteria and infiltrate gingival tissue. Propolis kelulut contains flavonoid that have an anti-inflammatory effect by suppressing the signal pathway p38 MAPK, JNK 1/2 and NF-kB that it can reduce the number of macrophage cells in inflammatory periodontal tissues. Objective: The purpose of this study was to determine the effect of 0.5 mg dose flavonoid propolis extract on the number of macrophage cells in gingiva wistar rats that have been made into a periodontitis condition. Method: This study used a pure experimental method with a post test only with control group design. There were 9 treatment groups, including flavonoid propolis extract on 1,3,5 days, ibuprofen gel on 1,3,5 days and negative control on 1,3 dan 5 days. Results: There was an effect of giving 0.5 mg flavonoids propolis kelulut extract to the number of macrophage cells in periodontitis. Conclusion: Flavonoid propolis kelulut extract has an effect in increasing the number of macrophage cells on day 3 and decreasing the number of macrophage cells on the 5th day.

Full Text:

PDF

References


Cekici A, Kantarci A, Hasturk H dan Dyke TE. Inflammatory and Immune Pathways In The Pathogenesis Of Periodontal Disease. Periodontology. 2014; 64 (1): 1-5

Nedzigora M, Kowalski J dan Gorska R. The Immune Respon In Periodontal Tissue. Arch Immunol Ther. 2017; 1(1): 2-6

Mills MP. Immunological and Inflammatory Aspects of Periodontal Disease. 2016; 1(1): 1-4

Astuti ND, Apriasari ML dan Nahzi MY. Efek Gel Ekstrak Batang Pisang Mauli (Musa Acuminata) Terhadap Jumlah Sel Makrofag Pada Inflamasi Pulpa. Dentino Jurnal Kedokteran Gigi. 2018; 3 (1): 38-41

Kalsum N, Sulaeman A, Setiawan B Dan Wibawan IW. Efek Propolis Cair Trigona Spp Terhadap Respon Imun Tikus Sprague Dawley Yang Diinduksi Sthapylococcus Aureus. Jurnal Gizi Pangan. 2017; 12 (1): 23-27

Wagh VD. Propolis : A Wonder Bees Product and Its Parmaco. Journal of Pharmacologycal Science. 2013; 13(2): 1-8

Dyke V dan Winkelhoff V. Infection And Inflammatory Mechanism. Journal Periodontol. 2013; 84 (4): 1-2

Quamilla N. Stres dan Kajian Periodontitis. Journal Of Syiah Kuala Dentistry Society. 2016; 1 (2): 163

Khawar S, A Sucheta dan Apoorva. Nsaids In Periodontology: Moving Beyond Analgesics. World Journal Of Pharmaceutical And Life Sciences. 2017; 3 (4): 226-227

Prabhu MN. Analgesics Use In Periodontal Surgery. International Journal Of Innovations In Dental Sciences. 2016; 1 (1): 2-6

Prasetya RC, Purwanti N Dan Haniastuti T. Infiltrasi Neutrofil Pada Tikus Dengan Periodontitis Setelah Pemberian Ekstrak Etanolik Kulit Manggis. Maj Kedokteran Gigi. 2014; 21 (1): 1-5

Scorfin JM. Biological Properties And Therapeutic Applications Of Propolis. Phytotherapy Research. 2016; 30 (1): 898-899

Setyadewi W, Oki AS dan Sunariani J. Moderate Intensity Exersice Effect On PMN And Machrophage Expression In Rattus Novergicus Post Tooth Extraction. Journal Of International Dental And Medical Research. 2017; 10 (2): 1-5

Jaya F, Radiati LE Dan Kalsum U. Pengaruh Pemberian Esktrak Propolis Terhadap Sistem Kekebalan Seluler Pada Tikus Putih (Rattus Novergicus) Strain Wistar. 2013: 1-6

Rismawati SN dan Ismiyati. Pengaruh Variasi pH Terhadap Kadar Flavonoid Pada Ekstraksi Propolis Dan Karakteristiknya Sebagai Antimikroba. Jurnal Konversi Universitas Muhammadiyah Jakarta. 2017; 6 (2): 1-5

Nurhayati W, Dewi V dan Djajakusumah T. Antiinflamatory Effect Of Trigona Spp Propolis In Restricting Edema Volume. Althea Medica Journal. 2015; 2 (1): 96-98

Sabir A dan Sumidarti A. Interleukin-6 Expressionon Inflamed Rat Dental Pulp Tissue After Capped With Trigona Sp. Propolis From South Sulawesi Indonesia. Saudi Journal Of Biological Sciences. 2017; 24(1): 1-4

Apriasari ML, Dachlan YP dan Ernawati DS. Potensi bahan alam terhadap penyembuhan ulser mukosa mulut. Salemba Medika. 2017: 28-31.

Sardana D, Indhusekar KR dan Manchanda S. Role Of Propolis In Dentistry: Review Of Literature. Focus On Alternative And Complementary Therapies. 2013; 18 (3): 118-121

Sabir A dan Sumidarti A. Interleukin-6 Expressionon Inflamed Rat Dental Pulp Tissue After Capped With Trigona Sp. Propolis From South Sulawesi Indonesia. Saudi Journal Of Biological Sciences. 2017; 24

Prasetya RC. Jumlah Sel Makrofag Gingiva Tikus Wistar Jantan Yang Diinduksi Periodontitis Setelah Pemberian Ekstrak Etanolik Kulit Manggis. Jurnal Dentofasial. 2013; 12 (3): 135-138

Rofiah. Pengaruh Propolis Terhadap Jumlah Makrofag Pada Luka Sayat, Studi Eksperimental Pada Kulit Mencit (Mus Musculus) Jantan Galur Balb/C. Sains Medika Journal Medicine of Health. 2014; 2 (3): 1-6

Izzaty A, Dewi N Dan Pratiwi D. Ekstrak Haruan (Channa Striata) Secara Efektif Menurunkan Jumlah Limfosit Fase Inflamasi Dalam Penyembuhan Luka. Dentofasial Jurnal. 2014; 13(3): 176-179

Farahmand A, Sayar F Dan Esfahani Bj. Clinical Eficaccy Of Subgingivally Delivered 2,5% Ibuprofen Gel In Chronic Periodontitis: A Randomized Controlled Clinical Trial. Journal Of International Oral Health. 2016; 8 (6): 651-654

Agustin R, Dewi N dan Rahardja SD. Efektivitas Ekstrak Ikan Haruan (Channa Striata) Danibuprofen Terhadap Jumlah Sel Neutrofil Pada Proses Penyembuhan Luka (Studi In Vivo Pada Mukosa Bukal Tikus (Rattus Novergicus) Wistar. Dentino Jurnal Kedokteran Gigi. 2016; 1 (1): 71-72




DOI: http://dx.doi.org/10.20527/dentino.v5i1.8117

DOI (PDF): http://dx.doi.org/10.20527/dentino.v5i1.8117.g6013

Article Metrics

Abstract view : 3270 times
PDF - 1886 times

Refbacks

  • There are currently no refbacks.


Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia

E-mail. [email protected]
Website. fkg.ulm.ac.id

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.