ANTIBACTERIAL ACTIVITY OF CHITOSAN FROM HARUAN (Channa striata) FISH SCALES AGAINST THE GROWTH OF Porphyromonas gingivalis

Rahma Dania A.J, Deby Kania Tri Putri, Irham Taufiqurrahman

Abstract


Background: Periodontal disease is one of the dental and oral diseases that is often found in people in Indonesia. RISKESDAS (2018) stated that the prevalence of people who have dental and oral health problems in Indonesia is 57.6%. The prevalence of oral and dental problems in South Kalimantan is 60%. Periodontal disease is damage to the supporting tissues of the teeth caused by specific microorganisms such as Porphyromonas gingivalis which are gram negative bacteria. The growth of these bacteria can be inhibited and killed by chitosan from haruan fish scales. Chitosan is a natural product of chitin, chitosan is the basic ingredient of fish scales. Chitosan has advantages and more safety levels, because it has an active group that will bind to microbes so chitosan can inhibit and kill microbial growth. Objective: to analyze the antibacterial activity of chitosan scales (Channa striata) on the growth of Porphyromonas gingivalis. Methods: This research used true experimental with randomized pre and post test with only control group design with eight treatments. Chitosan of haruan fish scales with liquid dilution method to obtain MIC and solid dilution test to obtain MBC. Results: The results showed that Minimum Inhibitory Concentration (MIC) of chitosan haruan scales was found at a concentration of 2.5% characterized by a decrease in absorbance value of 0.092 and Minimum Bactericidal Concentration found at a concentration of 20% characterized by the absence of Porphyromonas gingivalis. Conclusion: There is an inhibitory activity and a bactericidal activity from chitosan of haruan fish scales on Porphyromonas gingivalis.

Full Text:

PDF

References


Ananta A N H, S Ernie Maduratna, A Eka Fitria. Luas Daerah Terinflamasi Pada Jaringan Periodontal Pasien Diabetes Melitus Tipe 2 Dibandingkan Dengan Pasien Non-Diabetes Melitus. Periodontic Journal. 2012; 4 (2): 55-64.

Aziz N, Gufran Muhammad Fikri B, Pitoyo William U, Suhandi. Pemanfaatan Ekstrak Kitosan dari Limbah Sisik Ikan Bandeng di Selat Makassar pada Pembuatan Bioplastik Ramah Lingkungan. Hasanuddin Student Journal. 2017; 1 (1) : 56-61.

Afrianti, Sety La Ode M, Tina L. Analisis Faktor Risiko Kejadian Penyakit periodontal Pada Usia Dewasa Muda (20-44 Tahun) Di Puskesmas Poasia Kota Kendari Tahun 2017. JIMKESMAS (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat). 2018; 3 (2): 1-8.

Aliasghari A, Khorasgani Mohammad R, Vaezifar S, Rahimi F, Younesi H, Khoroushi M. Evaluation of antibacterial efficiency of chitosan and chitosan nanoparticles on cariogenic streptococci: an in vitro study. IRAN J MICROBIOL. 2016; 8 (2): 93-100.

Putri, D.K.T, Kriswandini, IL, Luthfi Muhammad, Characterization of Streptococcus sanguis molecular receptors for Streptococcus mutans binding molecules. Dental Journal. 2016; 49 (4) : 213-216.

Newman Michael G, Takei Henry H, Klokkevold Perry R. Classification of Diseases and Conditions Affecting the Periodontium. Carranza Fermin A, editor. Carranza’s Clinical Periodontology, 12th ed. St. Louis, Missouri; 2015. P.45-67.

Wahyuni A, Dewi N, Budiarti Y.N, Uji Efektifitas Antibakteri Sediaan Tunggal Dibandingkan Kombinasi Seduhan Daun Teh Hijau (Camellia sinesis) Dan Madu. Dentino Jurnal Kedokteran Gigi. 2016; 1 (2) : 113-118.

Visveswaraiah, P. M., Prasad, D, dan Johnson, S. Chitosan A Novel Way to Intervene in Enamel Demineralization - An in Vitro Study, Int. J. Curr. Microbiol. Appl. Sci. 2014; 3(11): 617–627.

Ifa L, Artiningsih A, Julniar, Suhaldin. Pembuatan Kitosan Dari Sisik Ikan Kakap Merah. Journal Of Chemical Process Engineering. 2018; 3 (1): 47-50.

Faridah F, Khafidzoh A, Mustikawati D, Anggraeni N, Dharmawan Y. Chitosan Pada Sisik Ikan Bandeng (Chanos Chanos) Sebagai Alternatif Pengawet Alami Pada Bakso. Jurnal Ilmiah Mahasiswa. 2012; 2 (2): 76-79.

Raafat, D, dan Sahl, H. G. Chitosan and Its Antimicrobial Potential – A Critical Literature Survey, Microb. Biotech. 2009; 2: 186–201.

Ezoddini A, Azam F, Yassaei A.N, dan Rouhi G. Effects of Chitosan on Dental Bone Repair, Health. 2011; 3 (4) : 200-205.

Bangalino H dan Akbar A Muhammad I. Pemanfaatan Sisik Ikan Bandeng Sebagai Bahan Baku Kitosan Dengan Metode Sonikasi Dan Aplikasinya Untuk Pengawet Makanan. Prosiding Seminar Hasil Penelitian (SNP2M). 2017. p.105-108.

Diah. Kontrol Plak Kemikal Dalam Pencegahan Gingivitis dan Periodontitis. Periodontic Journal. 2010; 1 (2) : 1-6.

Riski R dan Sami Fitriyanti J. Formulasi Krim Anti Jerawat Dari Nanopartikel Kitosan Cangkang Udang Windu (Penaeusmonodon). JF Fik Uinam. 2015; 3 (4) : 153-161.




DOI: http://dx.doi.org/10.20527/dentino.v5i1.8122

DOI (PDF): http://dx.doi.org/10.20527/dentino.v5i1.8122.g6018

Article Metrics

Abstract view : 3645 times
PDF - 2055 times

Refbacks

  • There are currently no refbacks.


Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia

E-mail. [email protected]
Website. fkg.ulm.ac.id

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.