Penyuluhan Asal Mula Teknologi Polymerase Chain Reaction Bagi Komunitas Minggu Raya (Bagian 2)

Tanto Budi Susilo, Oni soesanto, Dewi sri susanti, Arfan Eko Fahrudin, Eko Suhartono, Yuyun Hidayat, Sri Cahyo Wahjono, Muchamad Arief Soendjoto, Krisdianto Krisdianto

Abstract


Program kegiatan masyarakat (PKM) ini merupakan bagian edukasi publik terhadap masa endemi or post-covid-19. Ulasan PKM sebagai berikut ini; Setelah penentuan struktur DNA oleh Watson-Crick, setelah amplifikasi dan pengurutan DNA oleh Mullis dan Sanger, selanjutnya Human Genome Project mambaca 3,055 milyar urutan pasang basa manusia abad 21 ini. Bank-bank data DNA/protein atau informasi biologi telah berdiri seperti; NCBI, DDBJ dan EMBI telah memuat lebih 500 juta urutan DNA berbagai jenis species. Bank ini sumber genomics, proteomics, metabolomics dan economics sekaligus, pada masa depan. Bagi Carpentier dan Daudna bukan hanya sekedar jumlah urutan DNA yang luar biasa tetapi harta karun yang tidak ternilai harganya, mengapa? Karena dia tidak buta huruf membaca urutan DNA, karena dia mampu memilih dan memilah mana fragmen yang palindromics mana yang bukan. Mana yang genes musics mana yang bukan. Mana genes memory penyandi antibody mana yang bukan. Ringkasnya seluruh persoalan genetic tubuh manusia dapat dibaca, di Human Genom Project ini. Jadi, apakah kita tetap ingin menutup mata. Metode structural equation modelling (SEM) dengan uji pretest dan post test terhadap tulisan asal usul PCR ini. Hasil uji ditunjukan kepada 32 responden berumur kisaran 18 tahunan dan 12 responden berumur kisaran 19 tahunan, berturut-turut sebagai berikut; sangat mengerti (0), mengerti (80,55), kurang mengerti (19,45) dan tidak mengerti (0); dan sangat mengerti (4,16), mengerti (82,83), kurang mengerti (13,03) dan tidak mengerti (0). Respon terhadap urgensi epistemologi PCR, untuk yang merumur 19 tahunan lebih mengerti daripada responden yang berumur 18 tahun, walaupun perbedaannya tidak nyata.


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DOI: https://doi.org/10.20527/ilung.v3i3.11887

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