The Potential of Pasak Bumi as a Food Supplement to Improve Spermatogenesis in Mice Model of Malnutrition

Ida Yuliana; Triawanti Triawanti; Didik Dwi Sanyoto; Husnul Khatimah; Asnawati Asnawati; Rayatul Aminah

doi:10.20527/jbk.v7i1.10526

The Potential of Pasak Bumi as a Food Supplement to Improve Spermatogenesis in Mice Model of Malnutrition

Ida Yuliana, Triawanti Triawanti, Didik Dwi Sanyoto, Husnul Khatimah, Asnawati Asnawati, Rayatul Aminah

Abstract

Malnutrition causes disruption of spermatogenesis in children. Pasak Bumi has the potential to increase spermatogenesis due to malnutrition. Aimed of research is to proved Pasak Bumi as a food supplement to improve spermatogenesis disorders due to malnutrition in malnourished rats. The research method used analytical observational method by observing the histological preparations of the testes of malnourished rats. Observations using a binocular microscope were analyzed with Optilab Camera and Image Raster software. There are 5 groups of experimental: M: malnutrition without intervention; P1: malnutrition + standard feed + PB 7.5 mg/kgBW; P2: malnutrition + standard feed + PB 15 mg/kgBW; P3: malnutrition + standard feed + PB 22.5 mg/kgBW; P4: malnutrition + standard feed + PB 30 mg/kgBW; Research parameters were number of seminiferous tubules, primary spermatocytes and Leydig cells. Data analysis used the Anova One way test with a confidence level of 95%. The results showed number of seminiferous tubules, primary spermatocytes and Leydig cells in the M group was less than the P1-P4 group. This suggests malnutrition causes disruption of spermatogenesis. Conclusion the administration of Pasak Bumi extract at various doses increased spermatogenesis in malnourished.

Keywords

Pasak Bumi; malnutrition; seminiferous tubules; primary spermatocytes; Leydig cell

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References

Depkes RI. Data and Information on Indonesia's Health Profile 2017; Depkes; 2018.

Muzi-Filho H, Bezerra CG, Souza AM, Boldrini LC, Takiya CM, Oliveira FL, et al. Undernutrition affects cell survival, oxidative stress, Ca2+ handling and signaling pathways in vas deferens, crippling reproductive capacity. PLoS One. 2013; 8(7): e69682.

Herbert DC. Growth patterns and hormonal profile of male rats with protein-calorie malnutrition. Anat Rec. 1980; 197(3): 339–54.

Carvalho M, Mateus L, Afonso F, Van Harten S, Cardoso LA, Redmer DA, et al. Testicular angiogenic activity in response to food restriction in rabbits. Reproduction 2016; 20-5.

Russell, LD, Ettlin RA, SinhaHikim, AP; Clegg ED. Histological and histopathological evaluation of the testis. Clearwater. Cache River Press; 1990.

Rosida L. Pengaruh Pemberian Ekstrak akar Pasak Bumi (Eurycoma longifolia Jack.) Per Oral Terhadap Jumlah Sel Spermatogenik, Sel Sertroli dan Sel Leydig pada Mencit (mus musculus) Jantan Strain [penelitian eksperimental Laboratoris Laporan Penelitian]. Swiss: GDLHUB Pasca Sarjana Universitas Airlangga. Surabaya; 2013.

Hestianah EP. Gambaran Spermatogenesis Tubulus semineferus Mencit (Mus Muculus) Jantan Strain Swiss Setelah Pemberian Ekstrak Metanol Akar Pasak Bumi (Eurycoma longifolia Jack.) Per Oral Penelitian eksperimental laboratoris [laporan penelitian]. Surabaya: JIPTUNAIR Fakultas Veterinarr UNAIR; 2004.

Maria N. Pengaruh Akar Pasak Bumi (Eurycoma longifolia Jack) terhadap Spermatogenesis dan Kualitas Spermatozoa Mencit [skripsi]. Yogyakarta: Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Gadjah Mada Yogyakarta; 2003.

Bazer FW, Wu G, Spencer TE, Johnson GA, Burghardt RC, and Bayless K. Novel pathways for implantation and establishment and maintenance of pregnancy in mammals. Mol Human Reprod. 2010; 16: 135-52.

Wallace JM, Luther JS, Milne JS, Aitken RP, Redmer DA Rey- nolds LP, and Hay WW. Nutritional modulation of adolescent pregnancy outcome. A review. Placenta 2006; 27: 61-68.

Burdge GC and Lillycrop KA. Nutrition, epigenetics, and developmental plasticity: Implications for understanding human disease. Annu Rev Nutr. 2010; 30: 315–39.

Wu G. Amino acids: Metabolism, functions, and nutrition. Amino Acids. 2009; 37: 1–17.

Omar A.H. Ahmed-Fari, Maha Nasr, Rania F. Ahmed and Rofanda M. Bakeer. Beneficial effects of curcumin nanoemulsion on spermatogenesis and reproductive performance in male rats under protein deficient diet model: enhancement of sperm motility, conservancy of testicular tissue integrity, cell energy and seminal plasma amino acids content. Journal of Biomedical Science. 2017; 24:66 DOI 10.1186/s12929-017-0373-5.

Aitken J, Fisher H. Reactive oxygen species generation and human spermatozoa: The balance of benefit and risk. Bioessays 1994; 16: 259–67. [CrossRef].

Rae MT, Kyle CE, Miller DW, Hammond AJ, Brooks AN, Rhind SM: The Effects Of Undernutrition, In Utero, on Reproductive Function In Adult Male And Female Sheep. Anim Reprod Sci. 2002, 72: 63-71.

Agarwal A, Rana, M Qiu, E. AlBunni, H Bui AD, Henkel R. Role of oxidative stress, infection and inflammation in male infertility. Andrologia. 2018; 50: e13126. [CrossRef].

Aitken RJ, Clarkson JS. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J. Reprod. Fertil. 1987; 81: 459–69.

Smits R, Mackenzie-Proctor, R.; Fleischer, K.; Showell, M.G. Antioxidants in fertility: Impact on male and female reproductive outcomes. Fertil. Steril. 2010; 110: 578–80.

WG Goreja and A. Tongkat, Tree That Cures a Hundred Diseases. New York: Amazing Herbs Press; 2004.

Bhat, A. A. Karim. Tongkat Ali (Eurycoma longifolia Jack): a review on its ethnobotany and pharmacological importance. Fitoterapia. 81.

Farouk AE, Benafri A. Antibacterial activity of Eurycoma longifolia Jack. A Malaysian medicinal plant. Saudi Med J. 2007; 28: 1422-4.

Kuo PC, Damu AG, Lee KH, Wu TS. Cytotoxic and antimalarial constituents from the roots of Eurycoma longifolia. Bioorg Med Chem. 2004; 12: 537-44.

Ang HH, Lee KL, Kiyoshi M. Sexual arousal in sexually sluggish old male rats after oral administration of Eurycoma longifolia Jack. J Basic Clin Physiol Pharmacol. 2004; 15: 303-9.

Chan KL, Choo CY, Abdullah NR, Ismail Z. Antiplasmodial studies of Eurycoma longifolia Jack using the lactate dehydrogenase assay of Plasmodium falciparum. J Ethnopharmacol. 2004; 92: 223-7.

Sholikhah EN, Wijayanti MA, Nurani LH, Mustofa. Stage specificity of pasak bumi root (Eurycoma longifolia Jack) isolate on Plasmodium falciparum cycles. Med J Malaysia. 2008; 63 Suppl A:98-9.

DOI: http://dx.doi.org/10.20527/jbk.v7i1.10526