THE EFFECT OF RAMANIA LEAF (Bouea macrophylla Griff) EXTRACT GEL ON COLLAGEN FIBERS DENSITY IN INCISIONAL WOUND OF MALE WISTAR RATS

Dinda Rizkia, Irham Taufiqurrahman, Renie Kumala Dewi

Abstract


ABSTRACT

Background: Ramania leaf (Bouea macrophylla Griff) extract gel has secondary metabolites in the form of flavonoids, steroids, phenols and terpenoids which have a role as antioxidant. They will protect the body from excessive production of reactive oxygen species (ROS) by increasing endogenous antioxidants SOD, CAT and GPX, so that wound healing will not be inhibited and the process of collagen synthesis can run smoothly. Objective: To analyze the effect of ramania leaf extract gel that was applied topically with 5%, 10% and 15% concentration on collagen fibers density in incisional wound of male Wistar rats (Rattus norvegicus) on the 7th and the 14th day. Method: This research is a pure experimental study with a posttest-only control group design, using 24 rats which were divided into 4 groups: the treatment groups given ramania leaf extract gel of 5%, 10%, 15%, and the control group given placebo gel. The application of the extract gel was done once within 24 hours. The collagen level was measured with a spectrophotometer on the 7th and the 14th day. Results: Two-Way ANOVA test results on the 7th and the 14th day of each group showed a significant difference with p=0.000 (p<0.05). The Bonferroni Post-hoc Test showed a significant difference with p<0.05 between the placebo gel group and the groups of ramania leaf extract gel of 5%, 10%, 15% on the 7th and the 14th day. Conclusion: There is an effect of ramania leaf extract gel on collagen fibers density with the most effective concentration of 15%.


Keywords: Antioxidant, , Collagen, Ramania Leaf Extract Gel

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References


REFERENCES

Huda N, Febriyanti E, Laura DD. Edukasi Berbasis Nutrisi dan Budaya Pada Penderita Luka Kronis. Jurnal Pendidikan Perawatan Indonesia. 2018; 4(1): 1-12.

Febrianti N, Tahir T, Yusuf S. Study Literature Peran Epidermal Growth Factor dalam Proses Penyembuhan Luka. Jurnal Keperawatan Muhammadiyah. 2019; 4 (1): 8.

Sabirin IPR, Maskoen AM, Hernowo BS. Peran Ekstrak Etanol Topikal Daun Mengkudu (Morinda citrifolia L.) pada Penyembuhan Luka Ditinjau dari Imunoekspresi CD34 dan Kolagen pada Tikus Galur Wistar. MKB. 2013; 45 (4): 229.

Pramono WB, Leksana E, Satoto HH. Pengaruh Pemberian Ropivakain Infiltrasi Terhadap Tampilan Kolagen Di Sekitar Luka Insisi Pada Tikus Wistar. Jurnal Anestesiologi Indonesia. 2016; 8 (1): 3

Putri RR, Hakim RF, Rezeki S. Pengaruh Ekstrak Daun Tapak Dara (Catharanthus roseus) terhadap Jumlah Fibroblas pada Proses Penyembuhan Luka di Mukosa Oral. Journal Caninus Dentistry. 2017; 2 (1): 20-30.

Fitri L, Taufiqurrahman I, Irnamanda DH. Phytochemical And Cytotoxicity Testing Of Ramania Leaves (Bouea Macrophylla Griffith) Ethanol Extract Toward Vero Cells Using Mtt Assay Method. Dentino Jurnal Kedokteran Gigi. 2018; 3 (1): 52-54.

Huldani, Kaidah, S., Adiputro, D.L., Harun Achmad, Sukmana, B.I., Deby, D.K.T.P, Wasiaturrahmah, Y, Renie Kumala Dewi, Aspriyanto, D., Isnur Hatta, Winias, S., Pratiwi, A.R., Ernita sari1, Putra. A.P., Anastasya Deborah M. C Manik, Ketrin Zailin, Wardani, I.K. Effect of Total Cholesterol Levels and Triglycerides on Blood Pressure Hypertension Patients Overview against Puskesmas Banjar Ethnic Group in Cempaka Banjarmasin. Sys Rev Pharm 2020; 11(4): 384 389 A multifaceted review journal in the field of pharmacy.

Napanggala A, Susianti, Apriliana E. Pengaruh Pemberian Getah Tanaman Jarak Pagar (Jatropha curcas L.) Secara Topikal Terhadap Tingkat Kesembuhan Luka Iris Pada Tikus Putih Jantan Galur Sprangue dawley. Publikasi FK Lampung. Lampung. 2012; 3(5): 31-34.

Noora WF, Apriantia N, Saputra SR, Afifah B, Apriasari ML, Suhartono E. Oxidative Stress on Buccal Mucosa Wound in Rats and Rule of Topical Application of Ethanolic Extracts of Mauli Banana (Musa Acuminata) Stem. The Journal of Tropical Life Science. 2015; 5(2): 84-87.

Rahman A, Taufiqurrahman I, Edyson. Perbedaan Total Flavonoid Antara Metode Maserasi Dengan Sokletasi Pada Ekstrak Daun Ramania (Bouea macrophylla Griff). Dentino. 2017; 1 (1): 22-7.

Hasanah U, Yusriadi, Khumaidi A. Formulasi Gel Ekstrak Etanol Daun Kelor (Moringa oleifera Lam) sebagai Antioksidan. Online Journal of Natural Science. 2017; 6 (1): 46-57.

Arief H, Widodo MA. Peranan Stres Oksidatif pada Proses Penyembuhan Luka. Jurnal Ilmiah Kedokteran Wijaya Kusuma. 2018; 5(2): 22-29.

Kurnia PA, Ardhiyanto HB, Suhartini. Potensi Ekstrak Teh Hijau (Camellia sinensis) Terhadap Peningkatan Jumlah Sel Fibroblas Soket Pasca Pencabutan Gigi pada Tikus Wistar. e-Jurnal Pustaka Kesehatan. 2015. 3 (1): 122-127.

Fuadi MI, Elfiah U, Misnawi. Jumlah Fibroblas pada Luka Bakar Derajat II pada Tikus dengan Pemberian Gel Ekstrak Etanol Biji Kakao dan Silver Sulfadiazine. e-Jurnal Pustaka Kesehatan. 2015; 3 (2): 244-248.

Sucita RE, Hamid IS, Fikri F, Elziyad MT, Purnama. Ekstrak Etanol Kayu Secang (Caesalpinia Sappan L.) Secara Topikal Efektif Pada Kepadatan Kolagen Masa Penyembuhan Luka Insisi Tikus Putih. Jurnal Medik Veteriner. 2019; 2(2): 119-126.

Gonzalez ACO, Costa TF, Andrade ZA, Medrado ARAP. Wound Healing – A Literature Review. An Bras Dermatol. 2016; 91 (5): 614-20.

Primadina N, Basori A, Perdanakusuma DS. Proses Penyembuhan Luka Ditinjau Dari Aspek Mekanisme Seluler Dan Molekuler. Qanun Medika. 2019; 3 (1): 31-42.

Etika AN, Nurrahayu KI, Suhato IPS. Pengaruh Ekstrak Jahe (Zingiber Officinale Roscoe) Terhadap Jumlah Sel Fibroblas pada Tikus (Rattus norvegicus). Journal of Nursing Care & Biomolecular. 2017. 2(1): 10-14.

Yuza F, Wahyudi IA, Larnani S. Efek Pemberian Ekstrak Lidah Buaya (Aloe Barbadensis Miller) pada Soket Gigi terhadap Kepadatan Serabut Kolagen Pasca Ekstraksi Gigi Marmut (Cavia Porcellus). Majalah Kedokteran Gigi. 2014; 21(2): 127-135.

Purnama H, Sriwidodo, Ratnawulan S. Review Sistematik: Proses Penyembuhan Dan Perawatan Luka. Farmaka. 2017; 15 (2): 251-256.

Ismardianita E, Widyawati, Ellanora D, Rosalina W, Nofrike L, Khairani VY. The Effectiveness Methanol Extract Clausena excavate on Number of Fibroblast and Density of Collagen Fibers After Tooth Extraction. Journal of Dentomaxillofacial Science. 2019. 4 (3): 170-175.

Apriasari ML, Dachlan YP, Ernawati DS. Potensi Bahan Alam terhadap Penyembuhan Ulser Mukosa Mulut. Jakarta: Salemba Medika; 2017. p.28-31.

Dewantari DR dan Sugihartini N. Formulasi dan Uji Aktivitas Gel Ekstrak Daun Petai Cina (Leucaena glauca, Benth) sebagai Sediaan Obat Luka Bakar. Farmasains. 2015; 2 (5): 217-222.

Wahdaningsih S, Setyowati E.P, Wahyuoni S. Aktivitas Penangkap Radikal Bebas Dari Batang Pakis (Alsophila Glauca J. Sm) Free Radical Scavenging Activity Of (Alsophila Glauca J. Sm). Majalah Obat Tradisional. 2011; 16(3): 156 – 160.

Prasetya RC. Ekspresi dan Peran Siklooksigenase-2 dalam Berbagai Penyakit di Rongga Mulut. Stomatognatic (J. K. G. Unej). 2015; 12(1) : 16-19.




DOI: http://dx.doi.org/10.20527/dentino.v6i1.10648

DOI (PDF): http://dx.doi.org/10.20527/dentino.v6i1.10648.g7061

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