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TOXICITY TESTING OF CHITOSAN OF PAPUYU FISH SCALE (Anabas testudineus) TOWARD BHK-21 FIBROBLAST CELLS
Abstract
Background: Pulp capping is a treatment that aims to maintain the viability of the pulp tissue. Calcium hydroxide (Ca(OH)2 is a material that is often used for pulp capping procedures. However, this material does not provide a good adaptation to dentin. Therefore, an alternative material that is more biocompatible is needed, one of which is by using papuyu fish scale chitosan. papuyu fish as an alternative material must be proven to have no toxic effects. Purpose: to analyze whether the chitosan of papuyu fish scales (Anabas testudineus) is toxic to BHK-21 fibroblast cells using the MTT assay method. Methods: This study was a pure experimental study with a post-test-only design with a control group design consisting of 12 groups. The group given the chitosan treatment of papuyu fish scales consisted of 10 groups with concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% with 2 control groups namely control cells. and media control. Results: The results showed that the chitosan of papuyu fish scales did not have a toxic effect on BHK-21 fibroblast cells because the percentage value of cell viability of the entire treatment group was > 60% and the IC50 value was > 0.1%, which was 5.405 %. Conclusion: There was no toxic effect after administration of papuyu fish scales chitosan (Anabas testudineus) with concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100% on cells BHK-21 fibroblasts.
Keywords: BHK-21 fibroblast cells, Papuyu fish scale chitosan, Toxicity test
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Torneck CD, Torabinejad M. Biologi Jaringan Pulpa dan Jaringan Sekitar Akar. Diperoleh dari : Walton RE, Torabinejad M (editor). Prinsip dan Praktik Ilmu Endodonsi. Ed2.
Dania AJR, Putri DKT, Taufiqurrahman I. Antibacterial Activity of Chitosan from Haruan (Channa Striata) Fish Scales Againts the Growth of Porphyromonas gingivalis. Dentino Jur. Ked. Gigi. 2020; 5(1): 53-57.
Fanshuri MI, Putri DKT. 2021. Uji Toksisitas Kitosan Sisik Ikan Haruan (Channa striata) Terhadap Sel Fibroblas BHK-21 Secara In Vitro. Dentin Jur. Ked. Gigi. 2021; 1(1);1-5.
Mardja TE, Rahmi F, Rusmawati E, Adriany R, Murtiningsih, Herlina, Setijantim, Usia T. Riset Sitotoksik Campuran Ekstrak Daun Sirsak (Annona muricata L) dan Kulit Buah Manggis (Garcinia mangostana L) pada Sel Vero dan Amil12. J. Trop. Pharm. Chem. 2016; 3(4): 285.
Adiana I, Syafiar L. Penggunaan Kitosan Sebagai Biomaterial di Kedokteran Gigi. Dentika Dental Jurnal. 2014; 18(2): 190-191.
Izzati HN, Humaira N, Ni’mah L. Identifikasi Awal Pengaruh Konsentrasi NaOH Pada Pembuatan Kitosan dari Limbah Sisik Ikan Papuyu. Seminar Nasional Industri Kimia dan Sumber Daya Alam. 2018; 1(1); 32.
Sari DP, Nahzi MYI, Budiarti LY. Efektivitas Daya Hambat Ektrak Umbi Bawang Dayak Terstandarisasi Fenol Terhadap Pertumbuhan Enterococcus Faecalis. Dentino Jurnal Kedokteran Gigi. 2017; 1(1); 57.
Suherman B, Latif M, Dewi STR. Potensi Kitosan Kulit Udang Vannemei (Litopenaeus vannamei) Sebagai Antibakteri Terhadap Staphylococccus epidermidis, Pseudomonas aeruginosa, Propionibacterium agnes, Dan Escherichia coli Dengan Metode Difusi Cakram Kertas. Media Farmasi. 2018; XIV(1).
Sularsih. Pengaruh viskositas kitosan gel terhadap penggunaannya di proses penyembuhan luka. JMKG. 2013;2(1): 64-65.
Maulidah, Hasbullah ID, Panjaitan FUA. Biocompability Test Of Haruan Fish (Channa Striata) Bonehydroxyapatite To Fibroblast Cell As Periodontal Pocket Therapy. Jurnal Dentino. 2018; 3(2): 150.
Saputri MV, Carabelly AN, Firdaus, IWAK. Toxicity Test of The Mixed Mouthwash of Mauli Banana Stem and Basil Leaf Against Fibroblast Cell Study in Vitro. Dentino Jurnal Kedokteran Gigi. 2019; 4(2): 151.
Ningrum CDS, Saputera D, Arifin R. TOXICITY TEST OF BAY LEAF EXTRACT ON BHK-21 FIBROBLAST CELLS IN VITRO. Dentino. 2019; 4(2): 178-182.
Nurul LH, Widyarini S, Mursyidi A. Uji Sitotoksik dam Uji Kombinasi Fraksi Etil Asetat Ekstrak Etanol Akar Pasak Bumi (EURYCOMA LONGIFOLIA JACK.,) dan Doksorubisin pada sel Limfosit. J. Trop. Pharm. Chem. 2015; 3(2): 139-140.
Fikroh RA. Sintesis dan Uji Aktivitas Senyawa 2-Bromo-4 , 5-Dimetoksikalkon Terhadap Sel Kanker T47d Secara In Vitro. Journal Of Pharmaceutical Science And Medical Research. 2018; 1(1): 37–9.
Hartomo BT, Firdaus FG. Pemanfaatan Biomaterial Kitosan Dalam Bidang Bedah Mulut. B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahman. 2019; 6(1): 63-70
Inneke F, Irawan, Reni. 2018. Nanokitosan dari Sisik Ikan: Apikasinya Sebagai Pengemas Produk Perikanan. Sulawesi Utara. Lembaga Penelitiaan dan Pengabdian Kepada Masyarakat Universitas Sam Ratulangi.
Saputra B, Kresnoadi U. Toxicity test of shrimp shell (Litopenaeus Vannamei) chitosan as bone graft scaffold on BHK-21 fibroblas cell cultures. EurAsian Journal of BioSciences. 2020; 14(2): 3747-3751.
Ningrum CDS, Saputera D, Arifin R. TOXICITY TEST OF BAY LEAF EXTRACT ON BHK-21 FIBROBLAST CELLS IN VITRO. Dentino. 2019; 4(2): 178-182.
Putri DKT, Diah W, Oktiani BW, Sukmana PR. Synthesis and Characteristics of Chitosan from Haruan (Channa Striata) Fish Scales. Sys Rev PHarm 2020; 11(4): 15 20.
Senthilraja P dan Kathiresan K. In Vitro Cytotoxicity MTT Assay In Vero, Hepg2 And Mcf -7 Cell Lines Study Of Marine Yeast. Journal Of Applied PHarmaceutical Science. Maret 2015; 5(3): 80–4.
Duhan KB, Putri DKT, Beta W. Pengaruh Perendaman Kitosan Sisik Ikan Haruan Terhadap Struktur Email Gigi. Dentin: Jurnal Kedokteran Gigi. 2021; 5(2): 105-106.
DOI: http://dx.doi.org/10.20527/dentino.v9i1.18857
DOI (PDF): http://dx.doi.org/10.20527/dentino.v9i1.18857.g9871
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