TOXICITY TEST OF Eusideroxylon zwageri BARK EXTRACT BASED ON LIVER HISTOPATHOLOGY HYDROPIC DEGENERATION AND NECROSIS

Dhiya Salma Azminida, I Wayan Arya Krishnawan Firdaus, Juliyatin Putri Utami, Beta Widya Oktiani, Erida Wydiamala

Abstract


Background: Ironwood bark extract contains antioxidant properties such as flavonoids, phenolics, and proanthocyanidins, which can stabilize ROS in the body and help accelerate wound healing. The liver plays a role in nutrient metabolism, detoxification, and ROS production. The lack of antioxidants to neutralize excess ROS makes the liver vulnerable to damage. Purpose: This study aimed to determine that there was no toxic effect of giving ironwood bark extract (Eusideroxylon zwageri) doses of 1.250 mg/kg, 2.750 mg/kg, and 4.750 mg/kg on the liver of Wistar rats based on histopathological features of hydropic degeneration and necrosis. Methods: Pure laboratory experimental quantitative (true experimental) with a post-test-only research design with control group design and One Way Anova statistical test. Treatment was given to 4 groups, namely group (K) was given aquadest control, group (T1) was given ironwood bark extract at a dose of 1.250 mg/kgBW, group (T2) was given ironwood bark extract at a dose of 2.750 mg/kgBW, and group (T3) given ironwood bark extract at a dose of 4.750 mg/kgBW. Results: The results showed no significant difference between the four treatment groups based on the average percentage of hydropic degeneration and necrosis. Conclusion: There was no toxic effect of giving ironwood bark extract doses of 1.250 mg/kgBW, 2.750 mg/kgBW, and 4.750 mg/kgBW on the liver of Wistar rats based on histopathological appearance of hydropic degeneration and necrosis for 14 days.

Keywords


Eusideroxlon zwageri; Hydropic degeneration; Liver; Necrosis; Toxicity

Full Text:

PDF

References


Kementerian Kesehatan RI. Laporan nasional riskesdas. Jakarta: Balitbangkes; 2018. 195–274.

Amelinda CM, Handayani ATW, Kiswaluyo. Profil kesehatan gigi dan mulut berdasarkan standar WHO pada masyarakat Kecamatan Kaliwates Kabupaten Jember. Stomatognatic: Jurnal Kedokteran Gigi Unej. 2022; 19 (9): 37–44.

Utami NK, Amperawati M. Sediaan nanopartikel kitosan ekstrak ikan gabus (Channa striata) dan uji aktivitas albumin terhadap penyembuhan luka pasca pencabutan gigi. Jurnal Skala Kesehatan. 2020; 11 (1): 12–20.

Arief H, Widodo MA. Peranan stres oksidatif pada proses penyembuhan luka. Jurnal Ilmiah Kedokteran Wijaya Kusuma. 2018; 5 (2): 22–9.

Noor DI, Firdaus IWAK, Oktiani BW. Antibacterial effectivity test of ulin bark extract (Eusideroxylon zwageri) on the growth of Porphyromonas gingivalis. Dentino Jurnal Kedokteran Gigi. 2021; 1 (1): 37–43.

Maharani R, Fernandes A, Laksmita AN, Salama DM. Status riset ulin (Eusideroxylon zwageri Teijsm. Binn) di Indonesia. 1st ed. Bogor: IPB Press; 2021. 2-31.

Kusuma IW, Rahmini, Ramadhan R, Rahmawati N, Suwasono RA, Sari NM. Phytochemicals and antidiabetic activity of Eusideroxylon zwageri stem bark collected from East Kalimantan, Indonesia. IOP Conference Series: Earth and Environmental Science. 2018; 144 (1): 1–7.

Anggraito YU, Susanti R, Iswari RS, Yuniastuti A, Lisdiana, Nugrahaningsih, Habibah NA, Bintari SH. Metabolit Sekunder Dari Tanaman Aplikasi dan Produksi. Semarang: FMIPA UNNES; 2018. 1–95.

Hermawan KK, Herbani M, Wahyuningsih D. Pengaruh Pemberian Ekstrak Air Kombinasi Daun Jati Belanda, Kemuning, Murbei, dan Rimpang Bangle Terhadap Kadar SOD dan MDA Hepar Tikus Dengan Diet Tinggi Lemak. Jurnal Bio Komplementer Medicine. 2019; 6 (3): 205–15.

Maulina M. Zat-zat yang Mempengaruhi Histopatologi Hepar. Al-Muqsith, editor. Lhokseumawe: Unimal Press; 2018. 1–88.

Fitmawati F, Titrawani T, Safitri W. Struktur Histologi Hati Tikus Putih (Rattus norvegicus Berkenhout 1769) dengan Pemberian Ramuan Tradisional Masyarakat Melayu Lingga, Kepulauan Riau. Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi. 2019; 3 (1): 11–9.

Mariam F, Firdaus IWAK, Panjaitan FUA. Uji Efektivitas Ekstrak Kulit Batang Pohon Kayu Ulin (Eusideroxylon zwageri) Terhadap Aggregatibacter Actinomycetemcomitans. Dentin Jurnal Kedokteran Gigi. 2020; 4 (2): 43–8.

Salwa IN, Firdaus IWAK, Azizah A. Uji toksisitas Ekstrak Kulit Batang Ulin (Eusideroxylon zwageri) Terhadap Sel Fibroblas BHK-21 secara in vitro. Dentin Jurnal Kedokteran Gigi. 2021; 5 (3): 148–53.

Sabry MM, Ramadan NM, Al Dreny BA, Rashed LA, Abo El Enein A. Protective Effect of Apelin Preconditioning in A Rat Model of Hepatic Ischemia Reperfusion Injury; Possible Interaction Between the Apelin/APJ system, Ang II/AT1R system and eNOS. United European Gastroenterology Journal. 2019; 7 (5) :689–98.

Almahdy, Azlin N, Arifin H, Rivai H. Propolis Hepatoprotector Effect on Liver Damage of White Mice Induced by Valproic Acid. Journal of Pharmaceutical Sciences and Midicine. 2018; 3 (8): 1–12.

Parapasan SA, Muhartono, Iqbal M, Sukohar A. Pengaruh Ekstrak Etanol Buah Belimbing Wuluh (Averrhoa bilimbi L.) Sebagai Antioksidan Terhadap Histopatologi Hepar Tikus Putih (Rattus novergicus) Galur Wistar Yang Diinduksi Minyak Jelantah. Majority. 2023; 11 (2): 96–104.

Bladé C, Aragonès G, Arola-Arnal A, Muguerza B, Bravo FI, Salvadó MJ, Arola L, Suárez M. Proanthocyanidins in health and disease. BioFactors. 2016; 42 (1): 5–12.

Amer MA, Othman AI, EL-Missiry MA, Farag AA, Amer ME. Proanthocyanidins Attenuated Liver Damage and Suppressed Fibrosis in CCl4-treated rats. Environmental Science and Pollution Research. 2022; 29 (60): 91127–38.

Muhartono, Oktarlina RZ, Purohita NS. Pengaruh Pemberian Minuman Ringan Berkarbonasi Terhadap Gambaran Histopatologi Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Sprague Dawley. Majority. 2019; 8 (1): 71–7.

Makiyah SNN, Tasminatun S, Arsito PN, Fauziah KN, Nugrahanti DR, Putriani A. Subchronic Toxicity of Piperine in Piper Nigrum on the Histology of The Kidney, Liver, and Lungs of Mice (Mus musculus L.). Bali Medical Journal. 2021; 10 (3): 1161–7.

Prasetyo YE, Merdana IM, Kardena IM, Sudira IW. Gambaran Histopatologi Hepar Mencit yang Diberikan Ekstrak Etanol Sarang Semut. Buletin Veteriner Udayana. 2019; 11 (1): 44–50.

Shi S, Verstegen MMA, Mezzanotte L, de Jonge J, Löwik CWGM, van der Laan LJW. Necroptotic cell Death in Liver Transplantation and Underlying Diseases: Mechanisms and Clinical Perspective. Liver Transplantation. 2019; 25: 1091–1104.

Budianto A, Irwandi I, Muslihin A, Astuti R. Uji Toksisitas Subkronik Kombinasi Ekstrak Etanol Kunyit dan Jahe Terhadap Fungsi Hepar Tikus Putih. Jurnal Etnofarmasi. 2022; 1 (1): 1–6.

Hemanth I, Rao S, Nadoor P, Narayanaswamy HD, Satyanarayana ML, Jamuna K V. Histopathological Studies on Imatinib Mesylate Induced Toxicity in Wistar Rats and its Amelioration with Grape Seed Proanthocyanidins. The Pharma Innovation Journal. 2019; 8 (11): 1–6.

Wibawa IDGAP, Sumadewi KT, Cahyawati PN. Simvastatin Memperbaiki Degenerasi Hidropis dan Nekrosis Sel Hepatosit Mencit Subtotal Nefrektomi. Jurnal Bedah Nasional. 2022; 6 (1): 22–9.




DOI: http://dx.doi.org/10.20527/dentino.v9i2.20400

DOI (PDF): http://dx.doi.org/10.20527/dentino.v9i2.20400.g10409

Article Metrics

Abstract view : 86 times
PDF - 44 times

Refbacks

  • There are currently no refbacks.


Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia

E-mail. [email protected]
Website. fkg.ulm.ac.id

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.