THE EFFECT OF ACIDITY LEVEL AND SUBMERSION DURATION OF TEETH IN PEATLANDS TO DETERMINE BLOOD-GROUP ACCURACY THROUGH DENTAL PULP Study of postmortem blood-group identification technique through dental pulp
Abstract
Background: Indonesia has peatlands which spread all over the state and one of them is in Borneo
(Kalimantan). More than three million hectares of peatland spreads in South Borneo. Peatlands have relatively
high acidity level with pH range of 3-5. The peatlands in South Borneo is generally used for farming or public
cemetery. In certain situation, peatlands is often used as a dumping ground for criminal victims. Sometimes, the
authority finds it hard to identify the victim because the body is already decomposed. To identify the victim and
to analyse the cause of death, identification process is necessary. Teeth can be used to help the identification
process. Biological elements from the teeth namely dental pulp contains antigens that were useful to bloodgroups
determination by absorption elution method. Purpose: The objective of this research is to discover the
effect of peatlands acidity level and teeth submersions durations in determining blood-group accuracy from
dental pulp. Method: The method of this research used a quasi-experimental method to discover the effect of
peatlands acidity and pre-experimental method to discover the effect of teeth submersions duration. This
research used 48 pieces premolar teeth that were divided into 8 groups, control group, group submerged on
peatlands with pH 3,0-3,9, pH 4,0-4,9 and pH 5,0-5,9 to discover the effect of peatlands acidity and 1-day, 3-
day, 5-day and 7-day groups to discover the effect of teeth submersions time. Result: Fisher's Exact test results
showed p value 0,314 (p > 0,05) for the effect of peatlands acidity and p value 0,410 (p > 0,05) for the effect of
teeth submersions duration. Conclusion: It could be concluded that there are no effect of the peatlands acidity
and teeth submersions time to determine blood-group accuracy from dental pulp.
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Agus F, Subiksa IGM. Lahan gambut: potensi
untuk pertanian dan aspek lingkungan. Balai
penelitian tanah (badan penelitian dan
pengembangan pertanian). 2008: h. 34-38.
Noor YR, Heyde J. Pengelolaan lahan gambut
berbasis masyarakat di indonesia. 2007: h. 12-
Noorginayuwati. Kearifan budaya lokal dalam
pemanfaatan lahan gambut untuk pertanian di
kalimantan. Balai Penelitian Pertanian lahan;
(online),(http://balittra.litbang.pertanian.go.id/l
Dentino (Jur. Ked. Gigi), Vol II. No 2. September 2017 : 120 - 126
okal/Kearipan-2%20gina.pdf), di akses 13
Februari 2016.
Laporan Pelaksanaan Kegiatan Otopsi
Mayat/Kerangka. Kepolisian Negara Republik
Indonesia Daerah Kalimantan Selatan. Bid.
Kedokteran dan Kesehatan. 2013-2016.
Prawestiningtyas E, Algozi AM. Forensic
identification based on both primary and
secondary examination priority in victim
identifiers on two different mass disaster cases.
Jurnal Kedokteran Brawijaya. 2009; 22(2): 87-
Syafitri K, Auerkari E, Suhartono W. Metode
pemeriksaan jenis kelamin melalui analisis
histologis dan DNA dalam identifikasi
odontologi forensik. Jurnal PDGI. 2013; 62(1):
-16.
Pretty IA, Sweet D. A look at forensic
dentistry—part 1: the role of the teeth in the
determination of human identity. British dental
Journal. 2001; 190(7): 359-366.
Shetty M, Premalatha K. ABO blood grouping
from tooth materials. J Indian Acad Forensic
Med. 2010; 32(4): 336-338.
Ramnarayan BK, Maniunath M, Joshi AA.
ABO blood grouping from hard and soft tissues
of teeth by modified absorption-elution
technique. J Forensic Dent Sci. 2013; 5(1): 28-
Sasmita IS, Oscandar F, Effendi NH. Blood
type determination from extracted deciduous
teeth. Journal of Dentistry and Oral Hygiene.
; 6(4): 39-45.
Kansas City University of Medicine and
Biosciences. Department of Physiology. The
ABO system of typing blood. (online),
(http://courses.kcumb.edu/physio/btypes/abo_s
ystem.htm), diakses 26 Maret 2016.
Adhani, R., Sukmana, B. I., Suhartono, E.
Effect pH on demineralization dental
erosion. International Journal of Chemical
Engineering and Applications. 2015; 6(2): 138.
Walton ER, Torabinejad M. Prinsip dan praktik
ilmu endodonsia edisi 3. Alih bahasa; Narlan
Sumawinata. Jakarta: EGC, 2008. p. 23.
Vavpotič M., Turk T., Martinčič D. Š., Balažic,
J. Characteristics of the number of odontoblasts
in human dental pulp post-mortem. Forensic
science international. 2009; 193(1): 122-126.
Mehendiratta M., Jain K., Boaz K., Bansal M.,
Manaktala, N. Estimation of time elapsed since
the death from identification of morphological
and histological time-related changes in dental
pulp: An observational study from porcine
teeth. Journal of forensic dental sciences.
; 7(2): 95.
Srivastava SK, Daggolu PR, Burgess SC,
Minerick AR. Dielectrophoretic
characterization of erythrocytes: positive ABO
blood types. J Electrophoresis. 2008; 29: 5033-
Rijaldi F. Pengaruh suhu terhadap akurasi
penentuan golongan darah ABO dari pulpa
gigi. [Skripsi]. FKG Universitas Lambung
Mangkurat. 2016.
Fauziah S. Pengaruh lama perendaman gigi
dalam air laut terhadap akurasi penentuan
golongan darah abo melalui pulpa gigi
[Skripsi]. FKG Universitas Lambung
Mangkurat. 2016.
Hatakeyama T, Nagatomo H, Nobuyuki Y.
Interaction of the hemolitic lectin CEL-III from
the marine invertebrate (Cucumaria echinata)
with the erythrocyte membrane. The Journal of
Biological Chemistry. 1995; 270(8): 3560-64.
Mondal AG, Islam MA, Rahman MM, Begum
D, Sultana, MT. Role of blood group serology
in the detection, identification and
investigation for criminality in bangladesh.
Dinajpur Med Col J. 2011; 4(2): 83-88.
Ivanov IT. Low pH-induced hemolysis of
erythrocytes is related to the entry of the acid
into cytosole and oxidative stress on cellular
membranes. Biochimica et Biophysica Acta.
: 349-360.
Taylor AC. Responses of cells to pH changes
in the medium. The Journal of cell biology.
; 15(2): 201-209.
Şentürk, Ü. K., Gündüz, F., Kuru, O., Koçer,
G., Özkaya, Y. G., Yeşilkaya, A., Başkurt, O.
K. Exercise-induced oxidative stress leads
hemolysis in sedentary but not trained
humans. Journal of Applied Physiology. 2005;
(4): 1434-1441.
Berkovitz BKB, Moxham BJ, Linden RWA,
Sloan AJ. Oral biology. United Kingdom:
Elsevier, 2013. 142-194.
Y.F. Ren, Dental erosion: etiology, diagnosis
and prevention. (online), (www.rdhmag.com),
diakses 02 Januari 2017.
R. Lagocka, J.S. Bochinska, I. Nocen, K.
Jakubowska, M. Gora, J.B. Radlinska.
Influence of the mineral compotition of
drinking water taken from surface water
intake in enhancing regeneration processes in
Irnamanda: The Effect Of Acidity Level And Submersion Duration 125
mineralized human teeth tissue. Polish J of
Environ. Stud. 2011; 20(2): 411-416.
RP Parron, RP Carmichael, MA Marcon,
GKB. Sandor. Dental erosion in
gastroesophageal reflux disease. Journal of the
Canadian Dental Association. 2003; 69(2).
Aswath N, Selvamuthukumar S. C., Karthika
B. Role of dental pulp in identification of the
deceased individual by establishing ABO blood
grouping and Rhesus factor. Indian Journal of
Dental Research. 2012; 23(6): 811-813.
DOI: http://dx.doi.org/10.20527/dentino.v2i2.3949
DOI (PDF): http://dx.doi.org/10.20527/dentino.v2i2.3949.g3548
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