THE EFFECT OF ACIDITY LEVEL AND SUBMERSION DURATION OF TEETH IN PEATLANDS TO DETERMINE BLOOD-GROUP ACCURACY THROUGH DENTAL PULP Study of postmortem blood-group identification technique through dental pulp

Irnamanda DH, Iwan Aflanie, Meka Aulia Raban, Ainul Habibah

Abstract


Background: Indonesia has peatlands which spread all over the state and one of them is in Borneo
(Kalimantan). More than three million hectares of peatland spreads in South Borneo. Peatlands have relatively
high acidity level with pH range of 3-5. The peatlands in South Borneo is generally used for farming or public
cemetery. In certain situation, peatlands is often used as a dumping ground for criminal victims. Sometimes, the
authority finds it hard to identify the victim because the body is already decomposed. To identify the victim and
to analyse the cause of death, identification process is necessary. Teeth can be used to help the identification
process. Biological elements from the teeth namely dental pulp contains antigens that were useful to bloodgroups
determination by absorption elution method. Purpose: The objective of this research is to discover the
effect of peatlands acidity level and teeth submersions durations in determining blood-group accuracy from
dental pulp. Method: The method of this research used a quasi-experimental method to discover the effect of
peatlands acidity and pre-experimental method to discover the effect of teeth submersions duration. This
research used 48 pieces premolar teeth that were divided into 8 groups, control group, group submerged on
peatlands with pH 3,0-3,9, pH 4,0-4,9 and pH 5,0-5,9 to discover the effect of peatlands acidity and 1-day, 3-
day, 5-day and 7-day groups to discover the effect of teeth submersions time. Result: Fisher's Exact test results
showed p value 0,314 (p > 0,05) for the effect of peatlands acidity and p value 0,410 (p > 0,05) for the effect of
teeth submersions duration. Conclusion: It could be concluded that there are no effect of the peatlands acidity
and teeth submersions time to determine blood-group accuracy from dental pulp.


Full Text:

PDF

References


Agus F, Subiksa IGM. Lahan gambut: potensi

untuk pertanian dan aspek lingkungan. Balai

penelitian tanah (badan penelitian dan

pengembangan pertanian). 2008: h. 34-38.

Noor YR, Heyde J. Pengelolaan lahan gambut

berbasis masyarakat di indonesia. 2007: h. 12-

Noorginayuwati. Kearifan budaya lokal dalam

pemanfaatan lahan gambut untuk pertanian di

kalimantan. Balai Penelitian Pertanian lahan;

(online),(http://balittra.litbang.pertanian.go.id/l

Dentino (Jur. Ked. Gigi), Vol II. No 2. September 2017 : 120 - 126

okal/Kearipan-2%20gina.pdf), di akses 13

Februari 2016.

Laporan Pelaksanaan Kegiatan Otopsi

Mayat/Kerangka. Kepolisian Negara Republik

Indonesia Daerah Kalimantan Selatan. Bid.

Kedokteran dan Kesehatan. 2013-2016.

Prawestiningtyas E, Algozi AM. Forensic

identification based on both primary and

secondary examination priority in victim

identifiers on two different mass disaster cases.

Jurnal Kedokteran Brawijaya. 2009; 22(2): 87-

Syafitri K, Auerkari E, Suhartono W. Metode

pemeriksaan jenis kelamin melalui analisis

histologis dan DNA dalam identifikasi

odontologi forensik. Jurnal PDGI. 2013; 62(1):

-16.

Pretty IA, Sweet D. A look at forensic

dentistry—part 1: the role of the teeth in the

determination of human identity. British dental

Journal. 2001; 190(7): 359-366.

Shetty M, Premalatha K. ABO blood grouping

from tooth materials. J Indian Acad Forensic

Med. 2010; 32(4): 336-338.

Ramnarayan BK, Maniunath M, Joshi AA.

ABO blood grouping from hard and soft tissues

of teeth by modified absorption-elution

technique. J Forensic Dent Sci. 2013; 5(1): 28-

Sasmita IS, Oscandar F, Effendi NH. Blood

type determination from extracted deciduous

teeth. Journal of Dentistry and Oral Hygiene.

; 6(4): 39-45.

Kansas City University of Medicine and

Biosciences. Department of Physiology. The

ABO system of typing blood. (online),

(http://courses.kcumb.edu/physio/btypes/abo_s

ystem.htm), diakses 26 Maret 2016.

Adhani, R., Sukmana, B. I., Suhartono, E.

Effect pH on demineralization dental

erosion. International Journal of Chemical

Engineering and Applications. 2015; 6(2): 138.

Walton ER, Torabinejad M. Prinsip dan praktik

ilmu endodonsia edisi 3. Alih bahasa; Narlan

Sumawinata. Jakarta: EGC, 2008. p. 23.

Vavpotič M., Turk T., Martinčič D. Š., Balažic,

J. Characteristics of the number of odontoblasts

in human dental pulp post-mortem. Forensic

science international. 2009; 193(1): 122-126.

Mehendiratta M., Jain K., Boaz K., Bansal M.,

Manaktala, N. Estimation of time elapsed since

the death from identification of morphological

and histological time-related changes in dental

pulp: An observational study from porcine

teeth. Journal of forensic dental sciences.

; 7(2): 95.

Srivastava SK, Daggolu PR, Burgess SC,

Minerick AR. Dielectrophoretic

characterization of erythrocytes: positive ABO

blood types. J Electrophoresis. 2008; 29: 5033-

Rijaldi F. Pengaruh suhu terhadap akurasi

penentuan golongan darah ABO dari pulpa

gigi. [Skripsi]. FKG Universitas Lambung

Mangkurat. 2016.

Fauziah S. Pengaruh lama perendaman gigi

dalam air laut terhadap akurasi penentuan

golongan darah abo melalui pulpa gigi

[Skripsi]. FKG Universitas Lambung

Mangkurat. 2016.

Hatakeyama T, Nagatomo H, Nobuyuki Y.

Interaction of the hemolitic lectin CEL-III from

the marine invertebrate (Cucumaria echinata)

with the erythrocyte membrane. The Journal of

Biological Chemistry. 1995; 270(8): 3560-64.

Mondal AG, Islam MA, Rahman MM, Begum

D, Sultana, MT. Role of blood group serology

in the detection, identification and

investigation for criminality in bangladesh.

Dinajpur Med Col J. 2011; 4(2): 83-88.

Ivanov IT. Low pH-induced hemolysis of

erythrocytes is related to the entry of the acid

into cytosole and oxidative stress on cellular

membranes. Biochimica et Biophysica Acta.

: 349-360.

Taylor AC. Responses of cells to pH changes

in the medium. The Journal of cell biology.

; 15(2): 201-209.

Şentürk, Ü. K., Gündüz, F., Kuru, O., Koçer,

G., Özkaya, Y. G., Yeşilkaya, A., Başkurt, O.

K. Exercise-induced oxidative stress leads

hemolysis in sedentary but not trained

humans. Journal of Applied Physiology. 2005;

(4): 1434-1441.

Berkovitz BKB, Moxham BJ, Linden RWA,

Sloan AJ. Oral biology. United Kingdom:

Elsevier, 2013. 142-194.

Y.F. Ren, Dental erosion: etiology, diagnosis

and prevention. (online), (www.rdhmag.com),

diakses 02 Januari 2017.

R. Lagocka, J.S. Bochinska, I. Nocen, K.

Jakubowska, M. Gora, J.B. Radlinska.

Influence of the mineral compotition of

drinking water taken from surface water

intake in enhancing regeneration processes in

Irnamanda: The Effect Of Acidity Level And Submersion Duration 125

mineralized human teeth tissue. Polish J of

Environ. Stud. 2011; 20(2): 411-416.

RP Parron, RP Carmichael, MA Marcon,

GKB. Sandor. Dental erosion in

gastroesophageal reflux disease. Journal of the

Canadian Dental Association. 2003; 69(2).

Aswath N, Selvamuthukumar S. C., Karthika

B. Role of dental pulp in identification of the

deceased individual by establishing ABO blood

grouping and Rhesus factor. Indian Journal of

Dental Research. 2012; 23(6): 811-813.




DOI: http://dx.doi.org/10.20527/dentino.v2i2.3949

DOI (PDF): http://dx.doi.org/10.20527/dentino.v2i2.3949.g3548

Article Metrics

Abstract view : 256 times
PDF - 292 times

Refbacks

  • There are currently no refbacks.


Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia

E-mail. [email protected]
Website. fkg.ulm.ac.id

 


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.