.png)
ANALISIS SITOGENIK MIKRONUKLEUS MUKOSA BUKAL PADA PEROKOK AKTIF DAN PASIF
Abstract
ABSTRACT
Background: Smoking is well known as a habit that negatively impact both smokers and non-smokers but inhaling cigarette smoke or passive smokers. Nicotin compounds of cigarette and cigarette smoke are one of genotoxic compounds that will be nitrosated and form nitrosamines compound that may cause the damage of DNA. DNA damage which is caused by genotoxic compound will be manifested as micronucleus, a second nucleus smaller than the original nucleus. Purpose: This study aims to identify whether the number of micronucleus in active smokers was higher than passive smokers. Method: This study was observational research with cross-sectional approach with primary data, which was oral buccal mucosa swab of active and passive smokers. The total sample is 30 for each group. Results: The results of this study showed average 1,61. Mann Whitney± 1,81 and 14,47±number of active and passive smokers’ buccal micronucleus were 19,60 test results showed there was significant difference of number micronucleus between active and passive smokers (p=0,000).
Conclusion: Based on this study it can be concluded the number of buccal micronucleus on active smokers was higher than passive smokers
ABSTRAK
Latar belakang: Merokok merupakan salah satu kebiasaan yang dapat memberikan dampak negatif baik bagi penghisapnya maupun orang yang tidak merokok, tetapi menghirup asap rokok, atau biasa dikenal dengan perokok pasif. Kandungan nikotin pada rokok dan asap rokok merupakan salah satu senyawa genotoksik yang akan mengalami nitrosasi menjadi senyawa nitrosamin yang dapat menyebabkan kerusakan DNA. Kerusakan DNA akibat paparan genotoksik dapat bermanifestasi sebagai mikronukleus, yaitu inti sel kedua yang berukuran
lebih kecil dari inti sel sejati. Tujuan: Penelitian ini bertujuan untuk mengetahui apakah rata-rata jumlah mikronukleus pada perokok aktif lebih tinggi dibanding perokok pasif. Metode: Penelitian ini merupakan penelitian observasional dengan pendekatan cross-sectional yang menggunakan data primer yaitu preparat swab mukosa bukal dari kelompok perokok aktif dan pasif. Jumlah sampel masing-masing kelompok adalah 30 orang. Hasil: Hasil penelitian ini menunjukkan rata-rata jumlah mikronukleus mukosa bukal pada perokok aktif 1,61. Uji statistik Mann Whitney menunjukkan perbedaan yang± 1,81 dan 14,47 ±dan pasif adalah 19,60 signifikan antara perokok aktif dan pasif (p=0,000) .
Kesimpulan: Berdasarkan penelitian yang dilakukan dapat diambil kesimpulan bahwa rata-rata jumlah mikronukleus mukosa bukal pada perokok aktif lebih tinggi dibanding perokok pasif
Full Text:
PDFReferences
Bettendorfa O, Piffko J, Ba‗nkfalvi A. Prognostic and predictive factors in oral squamous cell cancer: important tools for planning individual therapy?. Oral Oncology 2004;40:110–119
Gandini S, Botteri E, Iodice S et al. Tobacco smoking and cancer: a meta-analysis. Int J Cancer 2008;122(1):155-164
Maroochio LS, Joelma L, Felipe FS, Lusiana C, dan Suzana OM. Oral squamous cell carcinoma: an analysis of 1,564 cases showing advances in early detection. J Oral Sci 2010;52(2):267-273
Warnakulasuria S, Thomas D, Michael MB, Elias CP, Phillip MP, Clemens W et al . Oral health risk of tobacco use and effect of cessation. International Dental Journal 2010;60:7-30
Lyon F. Tobacco smoke and involuntary smoking. IARC Monographs on the evaluation of carcinogenic risks to humans 2004(83)
Bartsch H, Rojas M, Nair U, Alexandrov K. Genetic cancer susceptibility and DNA adducts: studies in smokers, tobacco chewers, and coke oven workers. Cancer Detect Prev 1999;23(6):445-453
Palaskar and Jindal C. Evaluation of micronuclei using papanicolaou and may grunwald giemsa stain in individuals with different tobacco habits – a comparative study. J Clin Diag Res 2010;4:3607-3613
Kahsyap, Bina, Padala SR. micronucleus assay of exfoliated oral buccal cells: means to assess the nucleaur abnormalities in different disease. J Cancer Res Ther 2012;8(2):184-191
Keohavong P, Stephen GG. Molecular toxicology protocols. New Jersey: Humana Press Inc., 2005, p.69
Tolbert PE, C.M. Shy, J.W. Allen. Micronuclei and other nuclear anomalies in buccal smears: methods development. Mutat Res 1992;69-77.
Casartelli G, Bonatti S, De Ferrari M et al. Micronucleus frequencies in exfoliated buccal cells in normal mucosa, precancerous lesions and squamous cell carcinoma. Anal Quant Cytol Histol 2000;22:486-92
Naderi NJ, Sareh F, Samaneh S. Micronucleus assay of buccal mucosa cells in smokers with the history of smoking less and more than 10 years. Indian J Pathol Microbiol 2012;55:433- 438
Swarjana IK. Metodologi penelitian kesehatan: tuntunan praktis pembuatan proposal penelitian. Yogyakarta : ANDI, 2012, hal. 102
Gay LR, PL Diehl. Research Methods for Business and Management. Singapore : Prentice Hall, 1996
Batos-Aires D, Alvaro A, Maria LP, Daniel PM, Alexandra T. Preliminary study of micronuclei levels in oral exfoliated cells from patients with periodontitis. J Dent Sci 2013;8:200-204
Holland N, Claudia B, Micheline K-V, Bonassi S, Zeiger E, Knasmueller S et al. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the humn project perspective on current status and knowledge gaps. Mutat Res 2008:659;93-108
Jois HS, Alka DL dan Mohan K. Micronucleus as Potential Biomarker of Oral Carcinogenesis. IJDA 2010;2(2):197-202
Lodovici M dan Elisabetta B. Biomarkers of induced active and passive smoking damage. Int. J. Environ. Res. Public Health 2009;6:874-888
Pradeep MR, Yadavalli Guruprasad, Maji Jose, Kartikay Saxena, Deepa K, Vishnudas Prabhu. Comparative Study of Genotoxicity in Different Tobacco Related Habits using Micronucleus Assay in Exfoliated Buccal Epithelial Cells. J CDR 2014:8(5): ZC21- ZC24
Peterson LA. Formation, repair, and genotoxic properties of bulky DNA adducts formed from tobacco-spesific nitrosamines review article. J Nucleic Acid 2010:1-11
DeMarini, D. M. Genotoxicity of tobacco smoke and tobacco smoke condensate: a review. Mutation Research 2004;567:447-474
Goodsell DS. The molecular perspective: nicotine and nitrosamines. The Oncologist 2004;9:353-354
International Agency for Reseach on Cancer Monographs. Some tobacco-specific Nnitrosamines. Exposure Data, volumes 89
Kamath VV, Praveen A, Krishnanand S. Micronuclei as prognostic indicators in oral cytological smears: A comparison between smokers and non-smokers. J Clin Cancer Invest 2014;3(1):49-54
Batos-Aires D, Alvaro A, Maria LP, Daniel PM, Alexandra T. Preliminary study of micronuclei levels in oral exfoliated cells from patients with periodontitis. J Dent Sci 2013;8:200-204
N Sivasankari P, Sohinder K, Reddy KS, Vivekanandam S, Ramachandra RK. Micronucleus index: an early diagnosis in oral carcinoma. J Anant Soc India 2009;57(1):8-13.
DOI: http://dx.doi.org/10.20527/dentino.v1i1.410
DOI (PDF): http://dx.doi.org/10.20527/dentino.v1i1.410.g335
Article Metrics
Abstract view : 2212 timesPDF - 10385 times
Refbacks
- There are currently no refbacks.
Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia
E-mail. [email protected]
Website. fkg.ulm.ac.id
This work is licensed under a Creative Commons Attribution 4.0 International License.