THE EFFECT OF GIANT SNAKEHEAD(Channa micropeltes)EXTRACT ON THE NUMBER OF MACROPHAGE IN DIABETES MELITUS WOUND HEALING (Research report)

Khairunnida Khairunnida, Amy Nindia Carabelly, Maharani Lailyza Apriasari

Abstract


Background:Giant snakehead contains albumin and omega-6 fatty acids which play an important role in diabetes mellitus wound healing. Wounds in people with diabetes mellitus have low level of albumin, thus inhibiting the macrophage function. Objective: To prove the effect of giant snakehead extract of 16mL/Kg dose of rats’ weight toward the number of macrophage on wistar rats’ back wounds with diabetes mellitus in day 4, 8 and 14. Method and Materials: This study applied pure experimental method with posttest-only control design. This study 36 wistar rats as sample which divided into 9 groups; group of giant snakehead extract with 16mL/Kg dose of rats’ weight, positive control group using haruan fish extract with 13.54mL/Kg dose of rats’ weight, and negative control group using BR2 feed. Results: The test result of One-way ANOVAon day 4 (p=0.022), day 8 (p=0.251) and day 14 (0.028). The test result of Post-hoc LSD showedthat there wereno significant differences on day 4 and 14 (p>00.5) between the groups of the giant snakehead extract and haruan fish extract. There wassignificant difference (p<00.5) between groups of the giant snakehead extract and BR2 feed. Conclusion: It can be concluded that giant snakehead extract at 16 mL / kg dose administered orally may increase the number of macrophage in wistar rat (Rattusnovergicus) induced diabetes mellitus back woundon day 4 and decreased macrophage cell count onday 8 and day 14.

Keywords


Diabetes mellitus, giant snakehead extract, macrophage, wound healing

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References


Greenberg M.S, Glick M dan Ship J.A. Burkert’s Oral Medicine. Ed.11st. Bc Decker Inc. Hamilton. 2008; Hlm. 509

Kementerian Kesehatan. Riset Kesehatan Dasar. Kementerian Kesehatan RI. Jakarta. 2013; hlm. 64

Sari Y, Eman S, Hartono. Pengaruh Frekuensi Vibrasi terhadap Penyembuhan Luka Diabetes. Universitas Jenderal Soedirman Purwokerto.2016; Hlm. 2.

Sariningsih E. Gigi BusukdanPoket Periodontal SebagaiFokusInfeksi. Gramedia, Jakarta. 2014; Hlm. 147.

Mansur.MajalahKesehatan Muslim ‘Diabetes Mellitus’. Pustaka Muslim. DI. Yogayakarta. 2013; Hlm. 31.

Irwanda W.F, Andrie M danLuliana S. Uji Efek Penyembuhan Luka Fase Air Ekstrak Ikan Toman (Channamicropeltes) Pada Tikus Putih Jantan Wistar yang Diberi Luka Sayat. Jurnal Mahasiswa Farmasi UNTAN. 2015; 1 (1) : 1-15.

Sura G.M, Carabelly A.N, Apriasari M.L. Aplikasi Ekstrak Haruan (Channastriata) 100% pada Luka PunggungMencit (Musmusculus) Terhadap Jumlah Neutrofil dan Makrofag. Jurnal PDGI. 2013; 62 (2): 41-44.

Nicodemus, Andrie M dan Lulisana S. Uji Efek Penyembuhan Luka Sayat Ekstrak Ikan Toman (ChannaMicropeltes) Secara Oral Pada Tikus Putih Jantan Wistar. Jurnal Mahasiswa Farmasi Untan. 2014; 1. (1): 1-14.

Firlianty, Eddy S, Happy N, Hardoko dan Annasari M.Chemical Composition and Amino Acid Profile of Channidae Collected From Central Kalimantan, Indonesia. IEESE International Journal of Science and Technology (IJSTE). 2013; 2 (4) : 25-29.

Suhartono E dan Djati M.S. Radikal Bebas dan Intoksikasi diabetes melitusium. Pustaka Banua. Banjarmasin. 2014; Hlm. 21-23.

Tjay T.H dan Rhardja K. Obat-Obat Penting Khasiat Penggunaan dan Efek Samping. PT Alex Media Komputindi Gramedia. Jakarta. 2007; Hlm. 31

B.Halliwell dan J. Gutteridge. Free Radicals in Biology andiabetes melitusedicine, Ed 4th. Oxford University Press. New York. USA.2007; p. 138-143.

Serhan C.N. Resolution Phase of Inflammation: Novel Endogenous Anti-Inflammatory and Proresolving Lipid Mediators and Pathways. Annu. Rev. Immunol. 2007; 25 (1): 101–137.

SoepribadiIstiati. Regenerasi dan Penyembuhan Untuk Kedokteran Gigi. Sagung Seto. Jakarta. 2013; Hlm. 11-15

Winarsi H. Antioksidan Alamidan Radikal Bebas. Kanisius. Yogyakarta. 2007; Hlm. 55-56

Mutiara G, Nurdiana, Yulian W.U. EfektifitasHidrogelBinahong (Anrederacordifolia (Ten.) Steenis) terhadapPenurunanJumlahMakrofagpadaPenyembuhan Luka FaseProliferasiTikusPutih (Rattusnorvegicus) GalurWistarKondisiHiperglikemia. MajalahKesehatan FKUB.2015; 1(1) : 29-39.

Firliyanti.Vacuum Drying Albumin Powder of Snakehead (Channamicropeltes) Potential For Wound Healing from Central Kalimantan, Indonesia. International Journal of ChemTech Research. 2016; 9 (6): 283-289.

Prekattil S.J, Agarwal A. Male Infertility. Springer. New York. 2012; p. 229

Naccache P.H dan Lefebvre J.S. A straight neutrophil path to healthy aging?.Blood. 2014; 123 (2): 154–156.

Susanti R,Yuniastuti A, Iswari RS. Aktivitas Reactive Oxygen Species Makrofag Akibat Stimulasi Gel Lidah Buaya Pada Infeksi Salmonella typhimurium. Jurnal MIPA. 2012; 35 (1) : 2.

Omar N.M, Nur-shahidatul A.M.D.Y, Nur’aziyah Z, Ahmad M.Z. Bioconversion of ω-Fatty Acid from Giant Snakehead (Channamicropeltes) Fish Oil.Orient. J. Chem. 2014; 30 (3): 1133.

Suhartono E. Toksisitas Oksigen Reaktifdan Antioksidan di Bidang Kedokteran dan Kesehatan. Goyen Publishing, Yogyakarta. 2016; Hlm.13-82.

Kumar V, Cotran R.S dan Robbins S.L. 2007. Buku ajar patologi. Edisi 7. Vol1 . EGC. Jakarta. 2007; Hlm. 45-70.

Rahmawati G, Rachmawati F.N danWinarsih H. Aktivitas Superokisda Dismutase Tikus Diabetes yang Diberi Ekstrak Batang Kapulaga dan Glibenklamid. Jurnal Scripta Biologica.2014; 1 (3): 197-201.

Diana J dan Agnès L.Research Articel: Macrophages and b-cells are responsible for CXCR2-mediated neutrophil infiltration of the pancreas during autoimmune diabetes. EMBO Mol Med. 6 (2014): 1090-1104.

Landen N.X, Dongqing L, Mona S. Transition from inflammation to proliferation: a critical step during wound healing. Cellular and Molecular Life Sciences.2016; 73:3861–3885.

Hidayat F.K, Ulfa dan Kristianingrum D.S. Perbandingan Jumlah Makrofag pada Luka Insisi Full Thickness antara Pemberian Ekstrak Umbi BidaraUpas dengan NaCl pada Tikus Wistar Jantan. Journal of Agromedicine and medical sciences.2015; 1 (1): 9-12

Gurtner G.C danThorme C.H. Wound healing: Normal and abnormal. Ed. 6th. Grabb and Smith’s plastic surgery. 2007; p. 77-98

Ningrum D.I.L dan Abdulgani N. Pengaruh Pemberian Ekstrak Ikan Gabus (Channastriata) Pada Struktur Histologi Hati Mencit (Musmusculus) Hiperglikemik. 2014; 2 (1): 2337-3520.

Sayuti K danYenrina R. Antioksidan, Alami dan Sintetik.Andalas University Press. Padang. 2015; Hlm. 7-38.

Narwadiya S.C, Dhumne U.L, Sahare K.N, Tuman P.M, Meshram V.G dan Singh V.Serum Protein Level Changes in Dots A diabetes melitus in istered Patients of Nagpur District: A Case Study. India Asian J ExpBiolScienst. 2012; 3 (1): 251-254.

Akbar J.Potensi Dan Tantangan Budidaya Ikan Rawa (Ikan Hitaman Dan Ikan Putihan) Di Kalimantan Selatan. Unlam Press. Banjarmasin. 2014; Hlm. 92-93.

Murdani O.J, Mohhamd A danWintari T. UjiEfekPenyembuhan Luka Sayat Ekstrak IkanToman (Channa micropeltes) Secara Oral Pada Tikus Jantan Galur Wistar Yang Diinduksi Streptozotocin. Jurnal Mahasiswa Farmasi UNTAN. 2017; 1 (1) : 1-9.

Perry J.S, Lio-Chan W.J, Andrew L.K, Katherine N, Zhuo Y, Chyi S.H. District Contribution of Aire and Antigen-Presenting-Cell Subsets to the generation of self-Toerance in the Thymus. Elsevier Inc. 2014; 414-426.

Dwijayanti D.R, Djati M.S, Ibrahim M, Muhaimin R. The Potential of VipAlbumin to Chronic Inflammation in Type 2 Diabetes Mellitus Balb/C Rat Model. American Journal of Immunology. 2015; 11(2): 56-67.

Mustafa A, Sajuti H, Permatasari N danWidodo M.A. The Effect of Channa striataExtract on Total Amino Acid, Arginine, and LeucineConsentrationin Serum of Streptozotocin Induced Diabetic Rat. International Journal of Science and Technology (IJSTE). 2014; 3 (4): 22-27.




DOI: http://dx.doi.org/10.20527/dentino.v4i1.6168

DOI (PDF): http://dx.doi.org/10.20527/dentino.v4i1.6168.g5041

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