.png)
ANTIBACTERIAL EFFECT OF MAULI BANANA STEM EXTRACT, BASIL LEAF EXTRACT, AND THEIR COMBINATION ON Staphylococcus aureus
Abstract
ABSTRACT
Introduction: Mauli banana stem and basil leaf extracts have an antibacterial effect on Staphylococcus aureus because they contain flavonoid, saponin, alkaloid, tannin, and eugenol. 1% povidone iodine is commonly used as an antiseptic and gargle for recurrent aphthous stomatitis caused by Staphylococcus aureus. Prolonged use of 1% povidone iodine is notable to possess adverse effects. Objective: To prove the antibacterial effect of mauli banana stem extract with 25% concentration, basil leaf extract with 12.5% concentration, and mauli banana stem and basil leaf combination extract with 25%, 50%, and 75% concentration on Staphylococcus aureus and compared to 1% povidone iodine. Methods: This study was applying the true experimental design with post-test only and control group design which consisted of 6 treatments. Group I was given with Mauli banana stem extract with 25% concentration, group II with basil leaf extract with 12.5% concentration, group III, IV, and IV with mauli banana stem and basil leaf combination extract with 25%, 50%, and 70% concentration, respectively. Meanwhile, group VI was given with 1% povidone iodine as a positive control. Each treatment had 4 times repetition. Antibacterial effectivity was assessed from the inhibition zone measurement of Staphylococcus aureus growth using the diffusion method. Results: This study revealed that the mean inhibition zone formed in group I was 6-6.2 mm, group II was 6-6.2 mm, group III was 6-6.9 mm, group IV was 7.6-8 mm, group V was 8-8.5 mm, and group VI was 10.6-11.8 mm. Conclusion: The antibacterial efficacy of mauli banana stem extract at 25% concentration, basil leaf extract at 12.5% concentration, mauli banana and basil leaf combination extract at 25%, 50%, and 75% concentration on Staphylococcus aureus has been proven but not equal to 1% povidone iodine’s efficacy.
Keywords: Basil leaf extract, Diffusion method, Inhibition zone, Mauli banana stem extract, Staphylococcus aureus,.
Full Text:
PDFReferences
Thantawi A, Khairiati, Nova MM, Marlisa S, Bakar A. Stomatitis Apthosa Rekuren (SAR) Minor Multiple Pre Menstruasi. Odonto Dental Journal.2014 Desember: 1(2): 57-62.
Yuditha S. The Management of Recurrent Aphtous Stomatitis with Gastrointestinal Disease Associated with Helicobacter pylori. Jurnal Ilmiah dan Teknologi Kedokteran Gigi. 2017; 13(1): 8-11.
Sulistiani A, Hernawati S, Mashartini A. Prevalensi dan Distribusi Penderita Stomatitis Aphtosa Rekuren (SAR) di Klinik Penyakit Mulut RSGM FKG Universitas Jember Tahun 2014. e-Jurnal Pustaka Kesehatan. 2017 Januari: 5(1): 169-176.
Loon YK, Satari MH, Dewi W. Antibacterial Effect of Pineapple (Ananas comosus) extract towards Staphylococcus aureus. Padjajaran Journal of Dentistry. 2018: 30(1): 1-5.
Gnanamani A, Setyaseela MP, Hariharan P. In Vitro Profilling of Antimethicillin Resistant Staphylococcus aureus Activity Against Selected Type and Clinical Strains. Applied Microbiology. 2016 Maret; 62(3):.283-289.
Widyaningrum DRW, Putri DKT, Taufiqurrahman I. Antibacterial Activities of Chitosan In Haruan Fish Scales (Channa striata) To The Growth of Staphylococcus aureus. DENTINO. 2019 September; 4(2): 162-167.
Sinaredi BR, Pradopo S, Wibowo TB. Daya Antibakteri Obat Kumur Chlorhexidine, Povidone iodine, Fluoride Suplementasi Zinc terhadap Streptococcus mutans dan Porphyromonas gingivalis. Dental Journal. 2014 Desember; 47(4): 211-214.
Kanagalingam J. Practical Use of Povidone iodine Antiseptic in The Maintenance of Oral Health and in The Prevention and Treatment of Common Oropharyngeal Infections. International Journal of Clinical Practice. 2015 Agustus; 69(11): 1247-1255.
Rifdayani N, Budiarti LY, Carabelly AN. Perbandingan Efek Bakterisidal Ekstrak Mengkudu (Morinda citrifolia Liin) 100% dan Povidone Iodine 1% terhadap Streptococcus mutans In Vitro. Dentino Jurnal. 2014 Maret; 11(1): 1-6.
Ani N, Rohyani IS, Ustadz M. Pengetahuan Masyarakat tentang Jenis Tumbuhan Obat di Kawasan Taman Wisata Alam Madapangga Sumbawa. Jurnal Pijar MIPA. 2018 September; 13(2): 160-166.
Apriasari ML. Aktivitas Antifungi Ekstrak Etanol dan Metanol Batang Pisang Mauli 100%. Stomatognatic J.K.G Unej. 2015: 16-29.
Angelina M, Turnip M, Khotimah S. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum sanctum L.) terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus. Protobiont Journal. 2015; 4(1): 184-189.
Noor MA, Apriasari ML. Efektivitas Antibakteri Ekstrak Metanol Batang Pisang Mauli (Musa acuminata) dan Povidone iodine 10% terhadap Streptococcus mutans. Jurnal PDGI. 2014; 63(3): 78-83.
Larasati DA. Efek Potensial Daun Kemangi (Ocimum basilicum L) sebagai Pemanfaatan Handsanitizer. Majority Jurnal. 2016 Desember; 5(5): 124-128.
Puspitasari D, Apriasari ML. Effect of 25%, 37,5%, 50% Mauli Banana (Musa acuminata) Stem Extract as Mouthwash on Color Stability of Bulk Fill Resin Composite. Dentino Jurnal. 2018 September; 3(2): 168-173.
Gupta BM, Gupta R, Agarwal A, Goel S. Ocimum sanctum (medical plant) research: a scientometric assessment of global publications output during 2008-17. International Journal of Information Dissemination and Technology. 2018; 8 (2): 68.
Ornay AKD, Prehananto H, Dewi ASS. Growth Inhibition of Candida albicans And Power Kill Candida albicans Extract Basil Leave. Jurnal Wiyata. 2017; 4(1): 78-83.
Apriasari ML, Fadhilah A, Carabelly AN. Aktivitas Antibakteri Ekstrak Metanol Batang Pisang Mauli (Musa sp) terhadap Streptococcus mutans. Dentofasial Jurnal. 2013 Oktober; 12(3): 148-15.
Apriasari ML, Iskandar, dan Suhartono E.
Bioactive compounds and antioxidant activity
of methanol extract mauli bananas (Musa sp)
stem. International Journal of Bioscience, Biochemistry and Bioinformatics 2014; 4(2): 14. 110-115.
Safirah R, Widodo N, Budiyanto MAK. Uji Efektivitas Insektisida Nabati Buah Crescentia cujete dan Bunga Syzygium aromaticum terhadap Mortalitas Spodoptera litura secara In Vitro sebagai Sumber Belajar Biologi. Jurnal Pendidikan Biologi Indonesia. 2016; 2(3): 265-276.
Machado JA, Rebelo MA, Favaro LIL, Vila MMDC, Gerenutti M. In vitro evaluation of the antimicrobial potential association of Schinus Terebinthifolius Raddi and Syzygium aromaticum L. IOSR Journal of Pharmacy. 2012; 2(3): 442.
Harmely F, Deviarny C, Yenni WS. Formulasi dan Evaluasi Sediaan Edible Film dari Ekstrak Daun Kemangi (Ocimum americanum L) sebagai Penyegar Mulut. Jurnal Sains & Farmasi. 2014; 1(1): 38-47.
Kindangen OC, Yamlean PVY, Wewengkang DS. Formulasi Gel Antijerawat Ekstrak Etanol Daun Kemangi (Ocimum basilicum L) dan Uji Aktivitasnya terhadap Bakteri Staphylococcus aureus secara In Vitro. Pharmacon Jurnal. 2018 Agustus; 7(3): 283-293.
Aspriyanto D, Nirwana I, Budi HS. FGF-2 Expression and The Amount of Fibroblast in The Incised Wound of Rattus novergicus Induced with Mauli Banana (Musa acuminata) Stem Extract. Dental Journal. 2017 September; 50(3): 121-126.
El-baky RMA, Hashem ZS. Eugenol and Linalool: comparison of their antibacterial and antifungal activity. 2016: 10 (44): 32.
Hamad A, Jumitera S, Puspawiningtyas E, Hartanti D. Aktivitas Antibakteri Infusa Kemangi (Ocimum basicilum L) pada Tahu dan Daging Ayam Segar. Jurnal Inovasi Teknik Kimia. 2017;2(1):8-1.
Sukandar EY, Garman AN, Khairina C. Uji Aktivitas Antimikroba Kombinasi Ekstrak Perikarp Manggis (Garcinia mangostana L.) dan Kelopak Bunga Rosella (Hibiscus sabdariffa L.) terhadap Bakteri Penginfeksi Kulit. Acta Pharmaceutica Indonesia. 2014; 39(3): 57-62.
Othman L, Sleiman A, Abdel-Massih RM. Antimicrobial Activity of Polyphenols and Alkaloids in Middle Eastern Plants. Frontiers in Microbiology. 2019 Mei; 10: 1-28.
Mawan AR, Indriwati SE, Suhadi. Aktivitas Antibakteri Ekstrak Metanol Buah Syzygium polyanthum terhadap Pertumbuhan Bakteri Eschercia coli. Jurnal Bioeksperimen. 2018 Maret; 4(1): 64-68.
DOI: http://dx.doi.org/10.20527/dentino.v5i2.8953
DOI (PDF): http://dx.doi.org/10.20527/dentino.v5i2.8953.g6384
Article Metrics
Abstract view : 883 timesPDF - 862 times
Refbacks
- There are currently no refbacks.
Contact Us:
Faculty of Dentistry
Lambung Mangkurat University
Jalan Veteran No. 128 B Banjarmasin, Indonesia
E-mail. [email protected]
Website. fkg.ulm.ac.id
This work is licensed under a Creative Commons Attribution 4.0 International License.