Analysis of Purity and Concentration of DNA Isolated  in Dragonfly (Onychogomphus forcipatus)

Alfi Sophian; La Ode Nasir

doi:10.20527/wb.v14i2.13209

Analysis of Purity and Concentration of DNA Isolated in Dragonfly (Onychogomphus forcipatus)

Alfi Sophian, La Ode Nasir

Abstract

Analysis of the purity and concentration of DNA isolation results was carried out using samples of dragonflies (Onychogomphus forcipatus) species of insects. This research is important because there are many kinds of research in the molecular field that require good DNA isolation techniques to support the success of the research. The purpose of this paper is to provide information about the quality of the isolated DNA seen using the purity and concentration parameters. The extraction method uses the centrifuge column method. The isolated data were analyzed statistically using the average test. The results of DNA isolation showed that the average concentration value was in the range of 60.10 – 69.95. The analysis of the purity of the isolated DNA read at the A260/A280 ratio was in the range of 1.817 – 1.929, while the purity analysis at the A260/A230 ratio was in the range of 0.760 – 0.822. Based on the results of the study, it can be concluded that the analysis of the purity and concentration of DNA from the isolation of dragonfly samples was in a good category at the A260/A280 ratio, while the purity read at the A260/A230 ratio was below the value categorized as good.

Keywords

Analysis; DNA; Purity; Concentration; Ratio

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References

Andreas, E. Sumantri, C. Nuraini, H. Farajallah, A. Anggraeni, A. (2010). Identification of GH|AluI and GHR|AluI genes polymorphisms in Indonesian buffalo. Journal of Indonesian Tropical Animal Agriculture. 35: 215-221.

Corkill, G. Rapley, R. (2008). The Manipulation of Nucleic Acid: Basic Tools & Techniques in Molecular Biomethods Handbook. Ed ke-2. New York (US): Humana Press.

Eppendorf. (2016). Nucleic Acid Photometry. Check of critical parameters. Eppendorf AG• 22331 Hamburg• Germany• eppendorf@eppendorf.com• www.eppendorf.com

Karthika, P., Vadivalagan, C. Chinnapan, G. & Shanmugam, A. (2012). DNA Barcoding of selected dragonfly species (Libellulidae and Aeshnidae) for species authentication with phylogenetic assessment. European Journal of Experimental Biology, 2012, 2 (6):2158-2165.

Matlock, B. (2015). Assessment of Nucleic Acid Purity. Technical Note 52646. Thermo Fisher Scientific, Wilmington, MA, USA.

Phillips, P. & Swanson, B.J. (2018). A genetic analysis of dragonfly population structure. Ecology and Evolution, 8(14), 7206–7215. doi:10.1002/ece3.425.

Qiagen. (2014). For Extraction of Total Nucleic Acid From a Range of Food Sampel Types. In Dneasy Mericon Food Hand Book. Sample & Technology Assay. Www.qiagen.com.

Sambrook, J. Fritsch, F. Miniatis, T. (1989). Molecular Cloning Laboratory Manual. 3rd edition. New York (US): Cold Spring Harbor Laboratory Pr.

Sophian, A. (2021a). Short Communication: Analysis of purity and concentration of extracted DNA on salted fish processed food products. Asian Journal of Natural Product Biochemistry, 19(1). https://doi:10.13057/biofar/f190104

Sophian, A. (2021b). Detection of Species DNA in Chicken Meatball Products Using NGF Genes as Molecular Markers. BiosciED: Journal of Biological Science and Education, 2(2), 47–51. doi:10.37304/bed.v2i2.3422.

Sophian, A. (2021c). Species DNA Detection Using PGR Gene Genetic Markers in Chicken Nuggets. Indonesian Food Science & Technology Journal, 5(1), 17-20. Retrieved from https://online-journal.unja.ac.id/ifstj/article/view/14618.

Sophian, A. (2021d). DNA Isolation of Chicken Feathers from the Base of the Young Feathers, the Base of the Old Feathers, and the Tip of the Feathers. BIOEDUSCIENCE, 5(2), 104-108. https://doi.org/10.22236/j.bes/526211 .

Sophian, A., Purwaningsih, R., Muindar, Igirisa, E.P.J., & Amirullah, M.L. (2021). Short Communication: Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on crab processed food product samples. Asian Journal of Tropical Biotechnology, 18(1), 13–27. https://doi.org/10.13057/biotek/c180103.

Sophian, A. & Syukur, A. (2021). Analysis of Purity and Concentration of Isolated DNA in Making Raw DNA of Rat Species. Eruditio : Indonesia Journal of Food and Drug Safety, 1(2), 1–5. https://doi:10.54384/eruditio.v1i2.75.

Sutanta, M., Wulan, D. T., Nabila, Y., & Sophian, A. (2022). Application of Double Wash Technique for Species DNA Isolation in Soft Capsule Shell Samples. Indonesia Journal of Food and Drug Safety, 2(1), 14–19. Retrieved from https://eruditio.pom.go.id/index.php/home/article/view/78.

Wulan, D.T, Sutanta, M, Sophian, A. (2021). Short Communication: Comparison of two commercial DNA extraction kit to obtain high quality porcine DNA. Asian J Trop Biotechnol 18: 69-72. https://doi:10.13057/biotek/c180203

DOI: http://dx.doi.org/10.20527/wb.v14i2.13209