Development of Molecularly Imprinted Polymer (MIP) Modified Electrode for The Analysis of Fructose Content in Honey by Cyclic Voltammetry

Salma Hananda Putri, Pirim Setiarso

Abstract


This study aims to develop a Molecularly Imprinted Polymer (MIP)-modified electrode using cyclic voltammetry for the analysis of fructose in honey. The synthesis of the Non-Imprinted Polymer (NIP) was carried out via polymerization using methyl methacrylate (MMA) and ethylene glycol dimethacrylate (EGDMA) in the presence of a fructose template. The template extraction step from the NIP yielded a MIP with specific cavities selective for fructose. Characterization using Fourier Transform Infrared (FTIR) spectroscopy revealed an -OH group absorption peak at 3409.07 cm⁻¹ in the NIP, which was not observed in the MIP or the Blank Polymer (PB). Characterization by Scanning Electron Microscopy (SEM) revealed the morphology of the MIP with heterogeneous particles and sharp pores, in contrast to the PB, which is non-porous, and the NIP, which has homogeneous particles. The optimal electrode was prepared with a composition of agar; MIP; KCl in a ratio of 1:1:2 (w/w). Voltammetric analysis at pH 5 provided the best operating conditions with a deposition time of 5 seconds and a scan rate of 0.5 V/s. This electrode yielded a Limit of Detection (LOD) of 2.642 ppm, a Limit of Quantitation (LOQ) of 8.808 ppm, and a % recovery of 101.112%. A comparison of fructose analysis results in honey using cyclic voltammetry and UV-Vis revealed no statistically significant differences, (p-value (2-tailed) = 0.694 > 0.05). These results indicate that the MIP-modified electrode has good accuracy and can be used as an alternative method for fructose analysis in honey.

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DOI: http://dx.doi.org/10.20527/jstk.v20i2.26347

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Department of Chemistry
Faculty of Mathematics and Natural Sciences
Universitas Lambung Mangkurat
Jl. A. Yani KM. 36 Banjarbaru 70714, Indonesia
Email: [email protected]
ISSN: 1411-1616 E-ISSN: 2549-8215

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