Identifikasi Kandungan Senyawa Antioksidan Sebagai Agen Anti-Aging Pada Minyak Atsiri Cascara Kopi Robusta (Coffea canephora)

Naomi Aprisa, Diky Setya Diningrat

Abstract


Coffee cascara, often considered waste, contains bioactive compounds with antioxidant potential. This study aimed to identify antioxidant compounds in essential oil from Robusta coffee cascara green bean and evaluate their activity. Extraction was performed using the Soxhlet method with 96% ethanol, followed by GC-MS analysis, PubChem and PASS Online evaluation, and antioxidant testing using the DPPH method. A total of 20 compounds were identified, mainly volatile phenolics, alkaloids, and heterocyclics. Quinic acid showed the highest antioxidant potential, while the IC50 value of 15.78 mg/L indicated very strong antioxidant activity. These results suggest that cascara essential oil from Robusta coffee green bean is a promising anti-aging agent.

Keywords


Coffea cascara, Essential oil, Antioxidant, Anti-aging, DPPH

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References


Ahmad, Z., & Damayanti. (2018). Penuaan kulit : patofisiologi dan manifestasi klinis (skin aging : pathophysiology and clinical manifestation). Berkala Ilmu Kesehatan Kulit Dan Kelamin, 30(3). https://doi.org/https://doi.org/10.20473/bikk.V30.3.2018.208-215

BRMP: Badan Perakitan dan Modernisasi pertanian, Kementerian Pertanian. (2025). Tren 2025: peluang dan daya saing kopi. https://perkebunan.brmp.pertanian.go.id/berita/tren-2025-peluang-dan-daya-saing-kopi

Bare, Y., Kuki, A. D., Rophi, A. H., Krisnamurti, G. C., Lorenza, M. R. W. G., & Sari, D. R. T. (2019). Prediksi asam kuinat sebagai anti-inflamasi terhadap COX-2 secara virtual. Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 4(3), 124-129. https://doi.org/10.24002/biota.v4i3.2516

Esquivel, P., & Jiménez, V. M. (2012). Functional properties of coffee and coffee by-products. Food Research International, 46(2), 488–495. https://doi.org/10.1016/j.foodres.2011.05.028

de Sousa, D. P., Damasceno, R. O. S., Amorati, R., Elshabrawy, H. A., de Castro, R. D., Bezerra, D. P., & Lima, T. C. (2024). Essential oils: chemistry and pharmacological activities—part II. Biomedicines, 13(7). https://doi.org/10.3390/biomedicines12061185

Diniyah, N., & Lee, S.-H. (2020). Komposisi senyawa fenol dan potensi antioksidan dari kacang-kacangan: review. Jurnal Agroteknologi, 14(01), 91. https://doi.org/10.19184/j-agt.v14i01.17965

Hung, T. M., Na, M., Thuong, P. T., Su, N. D., Sok, D., Song, K. S., ... & Bae, K. (2006). Antioxidant activity of caffeoyl quinic acid derivatives from the roots of Dipsacus asper Wall. Journal of ethnopharmacology, 108(2), 188-192. https://doi.org/10.1016/j.jep.2006.04.029

Hussen, N., Abdulla, S., Ali, N., Ahmed, V., Hasan, A., & Qadir, E. (2025). Role of antioxidants in skin aging and the molecular mechanism of ROS: a comprehensive review. Aspects of Molecular Medicine, 1–12. https://doi.org/https://doi.org/10.1016/j.amolm.2025.100063

Idris, M., & Kasumawati, F. (2024). Validation of vitamin c analysis method and evaluation of antioxidant activity of various plant extracts from South Kalimantan using uv-vis spectrophotometer. Jurnal Ilmiah Berkala: Sains dan Terapan Kimia, 18(2). http://dx.doi.org/10.20527/jstk.v18i2.17595

Padidi, N., Wisdawati, E., & Baba, B. (2024). Formulasi pupuk organik limbah kulit kopi dengan penambahan tanaman penghasil nitrogen terhadap pertumbuhan bibit kopi robusta (Coffea canephora L.). Agroplantae, 13(1), 82–91. https://doi.org/https://doi.org/10.51978/agro.v13i1.811

Papaccio, F., Caputo, S., & Bellei, B. (2022). Focus on the contribution of oxidative stress skin aging antioxidants. Antioxidants, https://doi.org/https://doi.org/10.3390/antiox11061121

Pebrianti, D., Kristiawan, M. A., Qoiriyah, R. H., Kholisah, S. N., Fakhira, A. H., Azizah, L. N., Rakhmawati, C. D., Salim, M. H., Solikhah, A., Pramesti, R. A., Septiawati, R. P., Sulistyowati, M. I., & Rijal, M. A. S. (2023). Potential of collagen as an active ingredient in cosmetics. Berkala Ilmiah Kimia Farmasi, 10(2), 48–54. https://doi.org/10.20473/bikfar.v10i2.46518

Poláková, K., Bobková, A., Demianová, A., Bobko, M., Mesárošová, A., Jurčaga, L., Švecová, T., Lidiková, J., & Belej, Ľ. (2025). Bioactive compounds and heavy metals content in Cascara Coffea Arabica. Journal of Microbiology, Biotechnology and Food Sciences, 14(6), 1–6. https://doi.org/10.55251/jmbfs.11759

Prakoeswa, F. R. S., & Sari, W. A. (2022). Penuaan kulit dan terapi yang aman bagi geriatri: artikel review skin. Jurnal Sains Dan Kesehatan, 4(5), 557–568. https://doi.org/https://doi.org/10.25026/jsk.v4i5.1294 1

Rahmadini, F., Julianti, E., & Lubis, Z. (2020). Warna kulit dan komposisi kimia buah asam gelugur (Garcinia atroviridis Griffith et Anders.) pada tingkat kematangan yang berbeda. Agrointek, https://doi.org/10.21107/agrointek.v14i2.6159

Raihan, A., Illahi, A. K., Rokhimah, S., Elisa, T. P. P., & Maliza, R. (2023). Identification of bioactive solutions of corn silk (Zea mays L.) extract and biological activity test by bioinformatics. Jurnal Biologi Tropis, 23(1), 245–250. https://doi.org/10.29303/jbt.v23i1.5846

Revol-Cavalier, J., Quaranta, A., Newman, J. W., Brash, A. R., Hamberg, M., & Wheelock, C. E. (2024). The Octadecanoids: synthesis and bioactivity of 18-carbon oxygenated fatty acids in mammals, bacteria, and fungi. Chemical Reviews, 125(1), 1-90. https://doi.org/10.1021/acs.chemrev.3c00520

Rustiah, W., & Umriani, N. (2018). Antioxidant activity test from fruit extract kawista (Limonia acidissima) using uv-vis spectrofotometer. Indo.J.Chem.Res. 6(1), 534–537. https://doi.org/10.30598/ijcr.2018.6-wao

Romauli, N. D. M., Siahaan, F. T. K., Sagala, M., Sihombing, H. V., Ambarita, H., & Manurung, H. (2023). Comparative investigation on the nutritional value of fresh coffee pulp, cascara powder, and cascara sap from arabica, robusta, and liberica coffee. IOP Conference Series: Earth and Environmental Science, 1230(1), 1–12. https://doi.org/10.1088/1755-1315/1230/1/012153

Sholikhati, A., Sukoharjanti, B. T., & Rusidah, Y. (2023). Potensi ekstrak kopi (Coffea Sp.) sebagai antioksidan: review. Jurnal Medika Indonesia, 4(2), 30–38. ttps://doi.org/10.26751/.v4i2.2049

Sofwan, R. (2013). Bugar selalu di tempat kerja. Jakarta: PT Bhuana Ilmu Populer.

Sundoro, A. K., Syukur, M., Elisa, N., & Advistasari, Y. D. (2024). Penentuan nilai ic 50 ekstrak daun jambu (Syzygium aqueum) dengan metode DPPH (1, 1-difenil-2-pikrilhidrazil). Jurnal Ilmiah Farmasi, 13(2), 176–180. Retrieved from https://repository.stifar.ac.id/Repository/article/view/674

Syabila, F. A., Supriyanti, F. M. T., & Zackiyah, Z. (2024). Herb-fortified arabica cascara infusion as a functional beverage. Jurnal Natural Scientiae, 4(2), 1 10. https://doi.org/https://doi.org/10.20527/jns.v4i2.13445

Winahyu, D. A., Marcellia, S., & Diatri, M. I. (2021). Uji aktivitas antioksidan ekstrak kulit buah kopi robusta (Coffea canephora Pierre ex A.Foehner) dalam sediaan krim. Jurnal Farmasi Malahayati, 4(1), 82–92. https://doi.org/10.33024/jfm.v4i1.4470

Yang, J., & Park, M.-J. (2025). Antioxidant effects of essential oils from the peels of citrus cultivars. Molecules, 30(4), 833. https://doi.org/https://doi.org/10.3390/molecules30040833

Yasin, R. M., Fahira, K. T., & Alpandari, H. (2023). Pemanfaatan kulit kopi menjadi teh cascara bagi pengusaha Kopi Robusta Gunung Muria. Prosiding Seminar Nasional Pengabdian Kepada Masyarakat, 4(1), 01–10. Retrieved from https://journal.unj.ac.id/unj/index.php/snppm/article/view/39278




DOI: http://dx.doi.org/10.20527/wb.v18i1.24135

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