Guided inquiry improves undergraduate students psychomotor skills in gram staining practicum
Abstract
Gram staining of bacteria is an important practical material in Microbiology and Parasitology courses as part of Basic Nursing Science. This technique is widely used for the initial identification of pathogenic microorganisms. However, observations indicate that some students have not mastered the Gram staining technique optimally, leading to difficulties in interpreting microscopic findings. Until now, practical learning has been dominated by demonstration and one way instruction methods, so that the development of students' psychomotor skills has not been optimal. This study aims to evaluate the effectiveness of the guided inquiry method in improving the psychomotor skills of nursing students in Gram staining practical. The study used a quasi experimental design with a pretest–posttest control group design. The research subjects were students of the Nursing Study Program, semester II of the 2025/2026 Academic Year, consisting of two classes, namely the guided inquiry class (70 students) and the control class (72 students). Psychomotor skill data were analyzed based on the difference between posttest and pretest scores. The difference in psychomotor skill improvement between the two groups was analyzed using the Independent Samples t-test at a significance level of 0.05. The results showed a significant difference in the difference in psychomotor skill scores between the guided inquiry group and the control group, with the guided inquiry group having a higher average score. This indicates that guided inquiry learning is effective in improving students' psychomotor skills in Gram staining practicums.
Abstrak. Pewarnaan Gram pada bakteri merupakan materi praktikum penting dalam mata kuliah Mikrobiologi dan Parasitologi sebagai bagian dari Ilmu Keperawatan Dasar. This technique is widely used for the initial identification of pathogenic microorganisms, so mastering these psychomotor skills is essential for students to ensure the accuracy of clinical diagnoses. Namun, pengamatan menunjukkan bahwa sebagian mahasiswa belum menguasai teknik pewarnaan Gram secara optimal, sehingga menimbulkan kesulitan dalam menafsirkan temuan mikroskopis. Hingga saat ini, pembelajaran praktikum masih didominasi oleh metode demonstrasi dan pengajaran satu arah, sehingga pengembangan keterampilan psikomotorik mahasiswa belum optimal. Penelitian ini bertujuan untuk mengevaluasi efektivitas metode inquiry terarah dalam meningkatkan keterampilan psikomotor mahasiswa keperawatan pada praktikum pewarnaan Gram. Penelitian ini menggunakan desain kuasi-eksperimental dengan desain pretest–posttest kelompok kontrol. Subjek penelitian adalah mahasiswa Program Studi Keperawatan, semester II Tahun Akademik 2025/2026, yang terdiri dari dua kelas, yaitu kelas inquiry terarah (70 mahasiswa) dan kelas kontrol (72 mahasiswa). Data keterampilan psikomotor dianalisis berdasarkan selisih skor posttest dan pretest. Perbedaan peningkatan keterampilan psikomotor antara kedua kelompok dianalisis menggunakan Uji-t Sampel Independen pada tingkat signifikansi 0,05. Hasil menunjukkan adanya perbedaan yang signifikan pada selisih skor keterampilan psikomotor antara kelompok pembelajaran inquiry terarah dan kelompok kontrol, dengan kelompok pembelajaran berbasis penyelidikan terpandu memiliki skor rata-rata yang lebih tinggi. Hal ini menunjukkan bahwa pembelajaran berbasis inquiry terarah efektif dalam meningkatkan keterampilan psikomotorik siswa pada praktikum pewarnaan Gram.
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Adauyah, R., & Aznam, N. (2024). Guided inquiry learning model in chemistry education: A systematic review. Jurnal Penelitian Pendidikan IPA, 10(3), 77–87. DOI: https://doi.org/10.29303/jppipa.v10i3.6373
Algiani, S. R., Artayasa, I. P., Sukarso, A., & Ramdani, A. (2023). Application of guided inquiry model using self-regulated learning approach to improve student's creative disposition and creative thinking skill in biology subject. Jurnal Penelitian Pendidikan IPA, 9(1), 221–230. DOI: https://doi.org/10.29303/jppipa.v9i1.2836
Almira, A., Rachmawati, A., Jelita, I. N., & Nurlaili, Y. (2023). Evaluasi penerapan model pembelajaran inkuiri terbimbing dalam pembelajaran kimia: Suatu tinjauan sistematis literatur. arXiv e-prints, arXiv-2312.DOI: https://doi.org/10.48550/arXiv.2312.10090
Ami, M. S., Ma’rifah, H. L., & Sholihah, F. N. (2021). The effect of guided inquiry model through practicum learning on psychomotor ability of class XI MIA students in MAN 10 Jombang. Jurnal Biologi dan Pembelajarannya (JB&P), 8(1), 14–18. DOI: https://doi.org/10.29407/jbp.v8i1.15397
Anwar, Y. A. S. (2020). The multilevel inquiry approach to achieving meaningful learning in biochemistry course. Biochemistry and Molecular Biology Education, 48(1), 28–37. DOI: https://doi.org/10.1002/bmb.21309
Bachhawat, A. K., Pandit, S. B., Banerjee, I., Anand, S., Sarkar, R., Mrigwani, A., & Mishra, S. K. (2020). An inquiry-based approach in large undergraduate labs: Learning by doing it the “wrong” way. Biochemistry and Molecular Biology Education, 48(3), 227–235. DOI: https://doi.org/10.1002/bmb.21331
Chen, H., Loo, B. K. G., Tan, D. H. F., Chow, C. C.-T., & Lee, J. E. C. (2025). Design for learning through inquiry to enhance clinical reasoning among new nursing graduates at a tertiary referral centre in Singapore. Journal of Nursing Education and Practice, 15(7), 1–10. DOI: https://doi.org/10.63564/jnep.v15n7p1
Coico, R. (2006). Gram staining. Current Protocols in Microbiology, 1, A.3C.1–A.3C.2. DOI: https://doi.org/10.1002/9780471729259.mca03cs00
Demaria, M., Barry, A., & Murphy, K. (2019). Using inquiry-based learning to enhance immunology laboratory skills. Frontiers in Immunology, 10, Article 2510. DOI: https://doi.org/10.3389/fimmu.2019.02510
Durrant, R. J., Doig, A. K., Buxton, R. L., & Fenn, J. P. (2017). Microbiology education in nursing practice. Journal of Microbiology & Biology Education, 18(2), 10-1128. DOI: https://doi.org/10.1128/jmbe.v18i2.1224
Gilardi, S., & Lozza, E. (2009). Inquiry-based learning and undergraduates’ professional identity development: Assessment of a field research-based course. Innovative Higher Education, 34(4), 245–256. DOI: https://doi.org/10.1007/s10755-009-9109-0
Gupta, T., Burke, K., Mehta, A., & Greenbowe, T. J. (2015). Impact of guided-inquiry-based instruction with a writing and reflection emphasis on chemistry students’ critical thinking abilities. Journal of Chemical Education, 92(1), 32–38. DOI: https://doi.org/10.1021/ed500059r
Lee, J. L., & Jeong, Y. (2022). Assessing nurses' educational needs based on knowledge and importance of clinical microbiology. Journal of Korean Biological Nursing Science, 24(3), 150–160. DOI: https://doi.org/10.7586/jkbns.2022.24.3.150
Lipson, S. M., & Gair, M. (2011). The recording of student performance in the microbiology laboratory as a training, tutorial, and motivational tool. Journal of Microbiology & Biology Education, 12(1), 48–50. DOI: https://doi.org/10.1128/jmbe.v12i1.248
Mursali, S., Hastuti, U. S., Zubaidah, S., & Rohman, F. (2024). Guided inquiry with Moodle to improve students’ science process skills and conceptual understanding. International Journal of Evaluation and Research in Education, 13(3), 1875–1884. DOI: https://doi.org/10.11591/ijere.v13i3.27617
Nisa, E., Koestiari, T., Habibbulloh, M., & Jatmiko, B. (2018). Effectiveness of guided inquiry learning model to improve students’ critical thinking skills at senior high school. Journal of Physics: Conference Series. (Vol. 997, No. 1, p. 012049). IOP Publishing. DOI: https://doi.org/10.1088/1742-6596/997/1/012049
Norman-McKay, L., & Committee, A. M. U. C. G. (2018). Microbiology in nursing and allied health (MINAH) undergraduate curriculum guidelines: A call to retain microbiology lecture and laboratory courses in nursing and allied health programs. Journal of Microbiology & Biology Education, 19(1), 10-11128. DOI: https://doi.org/10.1128/jmbe.v19i1.1524
Pallant, J. (2020). SPSS survival manual: A step by step guide to data analysis using IBM SPSS (7th ed.). Routledge. DOI: https://doi.org/10.4324/9781003117407
Paray, A. A., Singh, M., Mir, M. A., & Kaur, A. (2023). Gram staining: A brief review. International Journal of Research and Review, 10(9), 336–341. DOI: https://doi.org/10.52403/ijrr.20230934
Periyannan, G. R. (2019). Bacterial cellobiose metabolism: An inquiry-driven, comprehensive undergraduate laboratory teaching approach to promote investigative learning. Biochemistry and Molecular Biology Education, 47(4), 438–445. DOI: https://doi.org/10.1002/bmb.21237
Pikoli, M., Buhungo, S., Musa, W. J., Tangio, J. S., Sihaloho, M., Lukum, A., & Kilo, A. K. (2025, July). The Effect of The Guided Inquiry Learning Model with Multiple Representations (Interpelasi) on Student Learning Outcomes and Retention on Salt Hydrolysis Material. In 2nd International Conference on Sciences, Mathematics, and Education 2023 (ICOSMED 2023) (pp. 537-544). Atlantis Press. DOI: https://doi.org/10.2991/978-2-38476-410-5_55
Purwati, G. A. M., Sudana, D. N., & Arini, N. W. (2020). Mathematics learning with guided inquiry model open-oriented problem solving improves student learning outcomes. International Journal of Elementary Education, 4(4), 454–463. DOI: https://doi.org/10.23887/ijee.v4i4.27198
Rambe, Y. A., Silalahi, A., & Sudrajat, A. (2020). The effect of guided inquiry learning model and critical thinking skills on learning outcomes. The 5th Annual International Seminar on Transformative Education and Educational Leadership (AISTEEL 2020). (pp. 151-155). Atlantis Press. DOI: https://doi.org/10.2991/assehr.k.201124.033
Ratnasari, J. (2016). Penerapan model pembelajaran inkuiri terbimbing berbasis kegiatan laboratorium untuk meningkatkan hasil belajar siswa pada materi elastisitas kelas X SMA Negeri 2 Sidoarjo. Jurnal Inovasi Pendidikan Fisika, 5, 3–4. Retrieved from https://ejournal.unesa.ac.id/index.php/inovasi-pendidikan-fisika/article/view/16273
Roche Allred, Z. D., Tai, H., Bretz, S. L., & Page, R. C. (2017). Using PyMOL to explore the effects of pH on noncovalent interactions between immunoglobulin G and protein A: A guided-inquiry biochemistry activity. Biochemistry and Molecular Biology Education, 45(6), 528–536. DOI: https://doi.org/10.1002/bmb.21066
Sadita, S. E., Zainil, M., Fitria, Y., & Rahmi, U. (2025). Pengaruh model pembelajaran inkuiri terbimbing terhadap aktivitas belajar dan kemampuan berpikir kritis siswa. Jurnal Penelitian Pendidikan IPA, 11(8), 601–608. DOI: https://doi.org/10.29303/jppipa.v11i8.11822
Saefi, M., Adi, W. C., Rahmasiwi, A., Maghfiroh, H., Setiawan, M. E., Adlini, M. N., & Rizalia, S. (2023). Teaching microbial analysis techniques for the characterisation of baker's yeast through the inquiry-based laboratory. JPBI (Jurnal Pendidikan Biologi Indonesia), 9(3), 282–292. DOI: https://doi.org/10.22219/jpbi.v9i3.29201
Sandars, J., & Murdoch-Eaton, D. (2017). Appreciative inquiry in medical education. Medical Teacher, 39(2), 123–127. DOI: https://doi.org/10.1080/0142159X.2017.1245852
Şen, Ş. (2024). Process oriented guided inquiry learning: A systematic review using bibliometric analysis. Biochemistry and Molecular Biology Education, 52(2), 188–197. DOI: https://doi.org/10.1002/bmb.21803
Septyowaty, R., & Syafii, M. (2023). Application of the guided inquiry model to improve psychomotor skills and interest in learning physics. Jurnal Penelitian Pendidikan IPA, 9(10), 8106–8113. DOI: https://doi.org/10.29303/jppipa.v9i10.4434
Sii, P., Verawardina, U., Arpan, M., & Sulistiyarini, D. (2017). Penerapan model pembelajaran inkuiri pada mata pelajaran KKPI terhadap kemampuan psikomotorik siswa. Jurnal Pendidikan Informatika dan Sains, 6(2), 166–176. Retrieved from https://www.researchgate.net/publication/337110747
Sinon, I. L. S., Sapari, K. R., & Allo, A. Y. T. (2022, December). The influence of guided inquiry learning model through experimental method for understanding the concept of students in calorie material. In Journal of Physics: Conference Series (Vol. 2392, No. 1, p. 012006). IOP Publishing. DOI: https://doi.org/ 10.1088/1742-6596/2392/1/012006
Sujana, I. G. (2020). Meningkatkan hasil belajar IPA melalui penerapan metode inkuiri terbimbing. Journal of Education Action Research, 4(4), 514–521. DOI: https://doi.org/10.23887/jear.v4i4.28651
Sulawanti, E. V., Ramdani, A., Bahri, S., & Merta, I. W. (2019). Pengaruh penerapan model pembelajaran inkuiri berbasis laboratorium terhadap kemampuan psikomotorik siswa. Jurnal Pijar MIPA, 14(3), 141–147. DOI: https://doi.org/10.29303/jpm.v14i3.1039
Walker, L., & Warfa, A.-R. M. (2017). Process oriented guided inquiry learning (POGIL®) marginally effects student achievement measures but substantially increases the odds of passing a course. PLoS ONE, 12(10), e0186203. DOI: https://doi.org/10.1371/journal.pone.0186203
DOI: http://dx.doi.org/10.20527/bino.v8i2.25954
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