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Phytochemical screening of Balinese grape (Vitis vinifera L. var. Alphonso Lavallee) peel extract: Resources local plant-based lessons in biology
Abstract
The utilization of local natural resources for contextual and practical-based learning is an innovative approach in biology education. This study aimed to conduct a phytochemical screening of Balinese grape (Vitis vinifera L. var. Alphonso Lavallee) peel extract and integrate the findings into plant-based biology lessons. A mixed-method approach with a one-group pretest–posttest design was applied. Phytochemical tests identified the presence of flavonoids, phenols, alkaloids, tannins, and terpenoids. The integration of results into laboratory-based lessons led to an increase in students’ average scores from 66.70 to 78.48, as well as improvement in laboratory skills, cultural awareness, and appreciation of local biodiversity. These findings demonstrate that Balinese grape peel extract is a valuable educational tool for enhancing understanding of secondary metabolites and promoting scientific literacy. The future impact of this research includes the potential for in vitro and in vivo studies to assess the extract’s biological activity, and the implementation of local plant-based learning models that support sustainable education and preservation of indigenous knowledge.
Abstrak. Pemanfaatan sumber daya alam lokal untuk pembelajaran kontekstual berbasis praktikum merupakan pendekatan inovatif dalam pendidikan biologi. Penelitian ini bertujuan untuk melakukan skrining fitokimia kulit anggur Bali (Vitis vinifera L. var. Alphonso Lavallee) serta mengintegrasikan hasilnya ke dalam pembelajaran berbasis sumber daya tumbuhan lokal. Penelitian ini menggunakan metode campuran dengan desain pra-eksperimen one-group pretest–posttest. Skrining fitokimia dilakukan terhadap senyawa flavonoid, fenol, alkaloid, tanin, dan terpenoid melalui uji pereaksi spesifik. Hasil skrining menunjukkan adanya kelima senyawa aktif tersebut, Integrasi hasil ke dalam pembelajaran laboratorium meningkatkan nilai rerata siswa dari 66,70 menjadi 78,48, serta memperkuat keterampilan laboratorium, kesadaran budaya, dan apresiasi terhadap biodiversitas lokal. Temuan ini menunjukkan bahwa penggunaan ekstrak kulit anggur Bali dapat meningkatkan literasi sains dan pemahaman peserta didik terhadap metabolit sekunder. Dampak jangka panjang dari penelitian ini mencakup potensi pengembangan studi in vitro dan in vivo untuk mengeksplorasi aktivitas biologis ekstrak serta penerapan pembelajaran berbasis sumber daya lokal yang mendukung pendidikan berkelanjutan dan pelestarian kearifan lokal.
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Adiansyah, R., Amin, A. M., Yani, A., & Safitri, D. (2023). The correlation between metacognitive awareness and cognitive learning outcomes based on gender of biology education students. Biosfer: Jurnal Pendidikan Biologi, 16(2), 244–259. DOI: https://doi.org/10.21009/biosferjpb.26765
Ambarwati, R., & Wilujeng, P. (2023). Development of electronic worksheet of biodiversity based on local wisdom and culture to enhance student’s learning motivation. Biosfer: Jurnal Pendidikan Biologi, 16(1), 82–99. DOI: https://doi.org/10.21009/biosferjpb.27894
Alamin, A., Nurhanurawati, N., Rohman, F., & Perdana, R. (2024). Development of learning e-modules based on contextual teaching and learning on the material of changing the form of energy to improve students' science literacy. Jurnal Penelitian Pendidikan IPA, 10(8), 6095-6103. DOI: https://doi.org/10.29303/jppipa.v10i8.8323
Astuti, N. K. W., Wartini, N. M., & Utama, I. M. S. (2023). Karakteristik ekstrak pewarna kulit anggur Bali (Vitis vinifera L. var. Alphonso Lavallee) pada perlakuan konsentrasi dan pH pelarut etanol. Media Ilmiah Teknologi Pangan, 9(2), 75–90. DOI: https://doi.org/10.24843/MITP.2022.v09.i02.p75
Atak, A., Göksel, Z., & Yılmaz, Y. (2021). Changes in major phenolic compounds of seeds, skins, and pulps from various Vitis spp. and the effect of powdery and downy mildew diseases on their levels in grape leaves. Plants, 10(12), 2554. DOI: https://doi.org/10.3390/plants10122554
El-Elimat, T., Alfeilat, J., Al-Sawalha, N. A., & Alali, F. Q. (2022). Grape (Vitis vinifera L.) phytochemicals and their biochemical protective mechanisms against leading pathologies. Food Chemistry, 372, 131091. DOI: https://doi.org/10.1016/j.foodchem.2021.131091
Elsherif, D. E., Safhi, F. A., Subudhi, P. K., Shaban, A. S., El-Esawy, M. A., & Khalifa, A. M. (2024). Phytochemical profiling and bioactive potential of grape seed extract in enhancing salinity tolerance of Vicia faba. Plants, 13(12), 1596. DOI: https://doi.org/10.3390/plants13121596
Fahmi, F., Fajeriadi, H., & Irhasyuarna, Y. (2021). Integrated science teaching materials with local wisdom insights to improve students' critical thinking ability. BIO-INOVED: Jurnal Biologi-Inovasi Pendidikan, 3(2), 123–130. DOI: https://doi.org/10.20527/bino.v3i2.14148
Fajri, P., Relita, H., Sari, M. P., Hakiem, N., & Setiawan, Y. G. (2022). A design of interactive learning applications for phytochemical screening in vocational college. Handbook of Research on Implementing Digital Reality and Interactive Technologies to Achieve Society 5.0, 65. DOI: https://doi.org/10.4018/978-1-6684-4854-0.ch004
Ghosh, S., & Kalpana, C. A. (2025). Sustainable utilization of grape waste by phytochemical and bioactivity assessment for nutraceutical application of sundried seeds and peel. Amerta Nutrition, 9(1), 146-153. DOI: https://doi.org/10.20473/amnt.v9i1.2025.146-153
Guslinda, G., Kurniaman, O., Firdaus, L. N., & Hadriana, H. (2024). Developing local wisdom-based teaching materials on environmental conservation to improve students' environmental literacy. JPBI (Jurnal Pendidikan Biologi Indonesia), 10(1), 15–26. DOI: https://doi.org/10.22219/jpbi.v10i1.36108
Krasteva, D., Ivanov, Y., Chengolova, Z., & Godjevargova, T. (2023). Antimicrobial potential, antioxidant activity, and phenolic content of grape seed extracts from four grape varieties. Microorganisms, 11(2), 395. DOI: https://doi.org/10.3390/microorganisms11020395
Kurćubić, V. S., Stanišić, N., Stajić, S. B., Dmitrić, M., Živković, S., Kurćubić, L. V., Živković, V., Jakovljević, V., Mašković, P. Z., & Mašković, J. (2024). Valorizing grape pomace: A review of applications, nutritional benefits, and potential in functional food development. Foods, 13(24), 4169. DOI: https://doi.org/10.3390/foods13244169
Ma, X. (2024). Effect of cognitive context-based modeling instructional strategies in biology concept teaching: An educational action study. Lecture Notes in Education Psychology and Public Media, 49, 103-112. DOI: https://doi.org/10.54254/2753-7048/49/20231809
Makkiyah, F. A., Rahmi, E. P., Arista, R. A., Marliani, N., & Nurcholis, W. (2024). Antioxidant potential and polyphenol content in different parts of Graptophyllum pictum extracts. Journal of Advanced Pharmaceutical Technology & Research, 15(2), 111–116. DOI: https://doi.org/10.4103/japtr.japtr_325_23
Maryam, S., Arsani, N. L. K. A., & Tangguda, S. (2022). Grape (Vitis vinifera L.) skin extract reduced levels of SGPT and SGOT and improved the liver tissue structure of Wistar rats (Rattus novergicus) fed a high-cholesterol diet. Bali Medical Journal, 11(3), 1404–1409. DOI: https://doi.org/10.15562/bmj.v11i3.3602
Mashigo, M., Risenga, I., & Dukhan, S. (2024). First-year health sciences students’ perspectives on indigenous knowledge and medical practices. African Journal of Research in Mathematics, Science and Technology Education, 28(3), 422-436. DOI: https://doi.org/10.1080/18117295.2024.2424697
Milella, L., De Rosso, M., Gasparro, M., Gigante, S., Debiase, G., Forleo, A., Marsico, G., Perniola, R., Tutino, M., Notarnicola, M., Velasco, R., & Flamini, R. (2023). Correlation between antioxidant and anticancer activity and phenolic profile of new Apulian table grape genotypes (V. Vinifera L.). Frontiers in Plant Science, 13, 1064023. DOI: https://doi.org/10.3389/fpls.2022.1064023
Okereke, I., Ogu, B., Olori, G., & Olori, C. (2024). Promoting students’ academic achievement on difficult biology concepts using context-based instructional method: Implication for reducing depression among students. Jurnal Penelitian dan Pembelajaran IPA, 10(2), 297-312. DOI: https://dx.doi.org/10.30870/jppi.v10i2.28784
Rahmawati, Y., Hartanto, O., Falani, I., & Iriyadi, D. (2022). Students' conceptual understanding in chemistry learning using PhET interactive simulations. Journal of Technology and Science Education, 12(2), 303-326. DOI: https://doi.org/10.3926/jotse.1597
Rastija, V., Drenjančević, M., Kujundžić, T., Zmaić, L., & Karnaš, M. (2024). The antidiabetic effect of grape skin extracts of selected indigenous croatian white grapevine varieties. Foods, 13(24), 4143. DOI: https://doi.org/10.3390/foods13244143
Sihombing, R. A., Anwar, S., Liu, S.-Y., Muslim, M., Winarno, N., & Sihombing, P. J. (2025). Integrating local wisdom into environmental education: A systematic review of ethnoscience research in Indonesia. Journal of Natural Science and Integration, 8(1), 57-74. DOI: http://dx.doi.org/10.24014/jnsi.v8i1.35762
Silva, L. C., Correia, A. F., Gomes, J. V. D., Romão, W., Motta, L. C., Fagg, C. W., ... & Fonseca-Bazzo, Y. M. (2022). Lycorine alkaloid and Crinum americanum L. (Amaryllidaceae) extracts display antifungal activity on clinically relevant Candida species. Molecules, 27(9), 2976. DOI: https://doi.org/10.3390/molecules27092976
Suganob, E. T., De Asis, A. A., Superio, R. R., Estoque, E. I., Genodia, A. M. P., & Superio, D. L. (2024). Shaping sustainable future: Practices, awareness, perceptions, and information sources of undergraduate biology students at a university in rural Philippines. Information Development, 02666669251339136. DOI: https://doi.org/10.1177/02666669251339136
Wahdah, N., Nugroho, K. A., & Jumadi, J. (2023). Enhance critical thinking skills in application of PjBL-STEM on fluids dynamics: A literature study. Jurnal Penelitian Pendidikan IPA, 9(6), 89-94. DOI: https://doi.org/10.29303/jppipa.v9i6.2743
DOI: http://dx.doi.org/10.20527/bino.v7i2.22239
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